Znt7 (Slc30a7)-deficient mice display reduced body zinc status and body fat accumulation

Liping Huang, Yiu Yu Yan, Catherine P. Kirschke, Erik R. Gertz, Kevin C K Lloyd

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

In vitro studies have demonstrated that ZNT7 is involved in transporting the cytoplasmic zinc into the Golgi apparatus of the cell for zinc storage or to be incorporated into newly synthesized zinc-requiring enzymes/proteins. To evaluate the physiological role of ZNT7, we created a mouse model of Znt7 deficiency by a gene-trap approach. Znt7-deficient mice were zinc-deficient based on their low zinc content in serum, liver, bone, kidney, and small intestine. In embryonic fibroblasts isolated from Znt7-deficient mice, cellular zinc was ∼50% that of wild-type controls. Znt7-deficient mice also displayed some classic manifestations of dietary zinc deficiency, such as reduced food intake and poor body weight gain. However, the mutant mice did not show any sign of hair abnormality and dermatitis that are commonly associated with dietary zinc deficiency. A radioactive feeding study suggested that Znt7-deficient mice had reduced zinc absorption in the gut resulting in decreased zinc accumulations in other organs in the body. The poor growth found in Znt7-deficient mice could not be corrected by feeding the mutant mice with a diet containing 6-fold higher zinc (180 mg/kg) than the suggested adequate intake amount (30 mg/kg). Furthermore, the reduced body weight gain of the mutant mice was largely due to the decrease in body fat accumulation. We conclude that ZNT7 has essential functions in dietary zinc absorption and in regulation of body adiposity.

Original languageEnglish (US)
Pages (from-to)37053-37063
Number of pages11
JournalJournal of Biological Chemistry
Volume282
Issue number51
DOIs
StatePublished - Dec 21 2007

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Adipose Tissue
Zinc
Fats
Weight Gain
Body Weight
Dermatitis
Adiposity
Golgi Apparatus
Fibroblasts
Nutrition
Liver
Hair
Small Intestine
Bone
Eating
Genes
Diet
Kidney
Bone and Bones
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Znt7 (Slc30a7)-deficient mice display reduced body zinc status and body fat accumulation. / Huang, Liping; Yan, Yiu Yu; Kirschke, Catherine P.; Gertz, Erik R.; Lloyd, Kevin C K.

In: Journal of Biological Chemistry, Vol. 282, No. 51, 21.12.2007, p. 37053-37063.

Research output: Contribution to journalArticle

Huang, Liping ; Yan, Yiu Yu ; Kirschke, Catherine P. ; Gertz, Erik R. ; Lloyd, Kevin C K. / Znt7 (Slc30a7)-deficient mice display reduced body zinc status and body fat accumulation. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 51. pp. 37053-37063.
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