ZIP1 is a synaptonemal complex protein required for meiotic chromosome synapsis

Mary Sym, JoAnne Engebrecht, G. Shirleen Roeder

Research output: Contribution to journalArticle

434 Citations (Scopus)

Abstract

ZIP1 is a novel meiosis-specific gene required for chromosome synapsis and cell cycle progression in S. cerevlsiae. zip1 strains undergo homologous chromosome pairing, but are defective in synaptonemal complex (SC) formation. The zip1 mutation confers a uniform arrest in meiosis prior to the first division. zip1 strains display nearly wild-type levels of commitment to melotic recombination; however, mature reciprocal recombinants are not formed until cells are released from meiotic arrest by return to growth medium. DNA sequence analysis of ZIP1 reveals structural homology to a number of proteins containing coiled coils. Immunofluorescence experiments using anti-ZIP1 antibodies demonstrate that the ZIP1 protein localizes to synapsed meiotic chromosomes but not to unsynapsed axial elements. Taken together, these data suggest that ZIP1 is a component of the central region of the SC. We propose a model in which ZIP1 acts as a molecular zipper to bring homologous chromosomes in close apposition.

Original languageEnglish (US)
Pages (from-to)365-378
Number of pages14
JournalCell
Volume72
Issue number3
DOIs
StatePublished - Feb 12 1993
Externally publishedYes

Fingerprint

Synaptonemal Complex
Chromosome Pairing
Chromosomes
Meiosis
Proteins
DNA Sequence Analysis
Genetic Recombination
Fluorescent Antibody Technique
Anti-Idiotypic Antibodies
Fasteners
DNA sequences
Cell Cycle
Mutation
Genes
Cells
Growth
Antibodies
Experiments

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

ZIP1 is a synaptonemal complex protein required for meiotic chromosome synapsis. / Sym, Mary; Engebrecht, JoAnne; Roeder, G. Shirleen.

In: Cell, Vol. 72, No. 3, 12.02.1993, p. 365-378.

Research output: Contribution to journalArticle

Sym, Mary ; Engebrecht, JoAnne ; Roeder, G. Shirleen. / ZIP1 is a synaptonemal complex protein required for meiotic chromosome synapsis. In: Cell. 1993 ; Vol. 72, No. 3. pp. 365-378.
@article{f56f6a609fdc465198ab5b52b9b28966,
title = "ZIP1 is a synaptonemal complex protein required for meiotic chromosome synapsis",
abstract = "ZIP1 is a novel meiosis-specific gene required for chromosome synapsis and cell cycle progression in S. cerevlsiae. zip1 strains undergo homologous chromosome pairing, but are defective in synaptonemal complex (SC) formation. The zip1 mutation confers a uniform arrest in meiosis prior to the first division. zip1 strains display nearly wild-type levels of commitment to melotic recombination; however, mature reciprocal recombinants are not formed until cells are released from meiotic arrest by return to growth medium. DNA sequence analysis of ZIP1 reveals structural homology to a number of proteins containing coiled coils. Immunofluorescence experiments using anti-ZIP1 antibodies demonstrate that the ZIP1 protein localizes to synapsed meiotic chromosomes but not to unsynapsed axial elements. Taken together, these data suggest that ZIP1 is a component of the central region of the SC. We propose a model in which ZIP1 acts as a molecular zipper to bring homologous chromosomes in close apposition.",
author = "Mary Sym and JoAnne Engebrecht and Roeder, {G. Shirleen}",
year = "1993",
month = "2",
day = "12",
doi = "10.1016/0092-8674(93)90114-6",
language = "English (US)",
volume = "72",
pages = "365--378",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "3",

}

TY - JOUR

T1 - ZIP1 is a synaptonemal complex protein required for meiotic chromosome synapsis

AU - Sym, Mary

AU - Engebrecht, JoAnne

AU - Roeder, G. Shirleen

PY - 1993/2/12

Y1 - 1993/2/12

N2 - ZIP1 is a novel meiosis-specific gene required for chromosome synapsis and cell cycle progression in S. cerevlsiae. zip1 strains undergo homologous chromosome pairing, but are defective in synaptonemal complex (SC) formation. The zip1 mutation confers a uniform arrest in meiosis prior to the first division. zip1 strains display nearly wild-type levels of commitment to melotic recombination; however, mature reciprocal recombinants are not formed until cells are released from meiotic arrest by return to growth medium. DNA sequence analysis of ZIP1 reveals structural homology to a number of proteins containing coiled coils. Immunofluorescence experiments using anti-ZIP1 antibodies demonstrate that the ZIP1 protein localizes to synapsed meiotic chromosomes but not to unsynapsed axial elements. Taken together, these data suggest that ZIP1 is a component of the central region of the SC. We propose a model in which ZIP1 acts as a molecular zipper to bring homologous chromosomes in close apposition.

AB - ZIP1 is a novel meiosis-specific gene required for chromosome synapsis and cell cycle progression in S. cerevlsiae. zip1 strains undergo homologous chromosome pairing, but are defective in synaptonemal complex (SC) formation. The zip1 mutation confers a uniform arrest in meiosis prior to the first division. zip1 strains display nearly wild-type levels of commitment to melotic recombination; however, mature reciprocal recombinants are not formed until cells are released from meiotic arrest by return to growth medium. DNA sequence analysis of ZIP1 reveals structural homology to a number of proteins containing coiled coils. Immunofluorescence experiments using anti-ZIP1 antibodies demonstrate that the ZIP1 protein localizes to synapsed meiotic chromosomes but not to unsynapsed axial elements. Taken together, these data suggest that ZIP1 is a component of the central region of the SC. We propose a model in which ZIP1 acts as a molecular zipper to bring homologous chromosomes in close apposition.

UR - http://www.scopus.com/inward/record.url?scp=0027502061&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027502061&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(93)90114-6

DO - 10.1016/0092-8674(93)90114-6

M3 - Article

VL - 72

SP - 365

EP - 378

JO - Cell

JF - Cell

SN - 0092-8674

IS - 3

ER -