Zinc is associated with the β subunit of DNA-dependent RNA polymerase of Bacillus subtilis

Shirley M. Halling, F. J. Sanchez-Anzaldo, Ryuji Fukuda, Roy H. Doi, Claude F. Meares

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61 Scopus citations

Abstract

The Bacillus subtilis DNA-dependent RNA polymerase holoenzyme and core enzyme each contain approximately two atoms of zinc per molecule. When the dissociated subunits of the enzyme are passed through a blue dextran-Sepharose affinity column, only the β subunit binds to the column. The total zinc content of the enzyme is tightly bound to the β subunit. Dialysis studies suggest that the two zinc ions differ in the strength of their association with the β subunit. The presence of zinc in β is consistent with several other lines of evidence which indicate that this subunit is directly involved in phosphodiester bond formation. The blue dextran-Sepharose column procedure should be useful in future studies of the dissociation and reassociation of the enzyme since the method is rapid and provides excellent recovery of the β subunit as well as the α and β′ subunits of the RNA polymerase.

Original languageEnglish (US)
Pages (from-to)2880-2884
Number of pages5
JournalBiochemistry
Volume16
Issue number13
StatePublished - 1977

ASJC Scopus subject areas

  • Biochemistry

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    Halling, S. M., Sanchez-Anzaldo, F. J., Fukuda, R., Doi, R. H., & Meares, C. F. (1977). Zinc is associated with the β subunit of DNA-dependent RNA polymerase of Bacillus subtilis. Biochemistry, 16(13), 2880-2884.