The Bacillus subtilis DNA-dependent RNA polymerase holoenzyme and core enzyme each contain approximately two atoms of zinc per molecule. When the dissociated subunits of the enzyme are passed through a blue dextran-Sepharose affinity column, only the β subunit binds to the column. The total zinc content of the enzyme is tightly bound to the β subunit. Dialysis studies suggest that the two zinc ions differ in the strength of their association with the β subunit. The presence of zinc in β is consistent with several other lines of evidence which indicate that this subunit is directly involved in phosphodiester bond formation. The blue dextran-Sepharose column procedure should be useful in future studies of the dissociation and reassociation of the enzyme since the method is rapid and provides excellent recovery of the β subunit as well as the α and β′ subunits of the RNA polymerase.
|Original language||English (US)|
|Number of pages||5|
|State||Published - 1977|
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