Zinc is associated with the β subunit of DNA-dependent RNA polymerase of Bacillus subtilis

Shirley M. Halling, F. J. Sanchez-Anzaldo, Ryuji Fukuda, Roy H. Doi, Claude F. Meares

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The Bacillus subtilis DNA-dependent RNA polymerase holoenzyme and core enzyme each contain approximately two atoms of zinc per molecule. When the dissociated subunits of the enzyme are passed through a blue dextran-Sepharose affinity column, only the β subunit binds to the column. The total zinc content of the enzyme is tightly bound to the β subunit. Dialysis studies suggest that the two zinc ions differ in the strength of their association with the β subunit. The presence of zinc in β is consistent with several other lines of evidence which indicate that this subunit is directly involved in phosphodiester bond formation. The blue dextran-Sepharose column procedure should be useful in future studies of the dissociation and reassociation of the enzyme since the method is rapid and provides excellent recovery of the β subunit as well as the α and β′ subunits of the RNA polymerase.

Original languageEnglish (US)
Pages (from-to)2880-2884
Number of pages5
JournalBiochemistry
Volume16
Issue number13
StatePublished - 1977

Fingerprint

Bacilli
DNA-Directed RNA Polymerases
Bacillus subtilis
Zinc
Enzymes
Holoenzymes
Dialysis
Association reactions
Ions
Recovery
Atoms
Molecules
blue dextran-sepharose

ASJC Scopus subject areas

  • Biochemistry

Cite this

Halling, S. M., Sanchez-Anzaldo, F. J., Fukuda, R., Doi, R. H., & Meares, C. F. (1977). Zinc is associated with the β subunit of DNA-dependent RNA polymerase of Bacillus subtilis. Biochemistry, 16(13), 2880-2884.

Zinc is associated with the β subunit of DNA-dependent RNA polymerase of Bacillus subtilis. / Halling, Shirley M.; Sanchez-Anzaldo, F. J.; Fukuda, Ryuji; Doi, Roy H.; Meares, Claude F.

In: Biochemistry, Vol. 16, No. 13, 1977, p. 2880-2884.

Research output: Contribution to journalArticle

Halling, SM, Sanchez-Anzaldo, FJ, Fukuda, R, Doi, RH & Meares, CF 1977, 'Zinc is associated with the β subunit of DNA-dependent RNA polymerase of Bacillus subtilis', Biochemistry, vol. 16, no. 13, pp. 2880-2884.
Halling SM, Sanchez-Anzaldo FJ, Fukuda R, Doi RH, Meares CF. Zinc is associated with the β subunit of DNA-dependent RNA polymerase of Bacillus subtilis. Biochemistry. 1977;16(13):2880-2884.
Halling, Shirley M. ; Sanchez-Anzaldo, F. J. ; Fukuda, Ryuji ; Doi, Roy H. ; Meares, Claude F. / Zinc is associated with the β subunit of DNA-dependent RNA polymerase of Bacillus subtilis. In: Biochemistry. 1977 ; Vol. 16, No. 13. pp. 2880-2884.
@article{dddc51cec0ca4e7f805fa8c467eca1f3,
title = "Zinc is associated with the β subunit of DNA-dependent RNA polymerase of Bacillus subtilis",
abstract = "The Bacillus subtilis DNA-dependent RNA polymerase holoenzyme and core enzyme each contain approximately two atoms of zinc per molecule. When the dissociated subunits of the enzyme are passed through a blue dextran-Sepharose affinity column, only the β subunit binds to the column. The total zinc content of the enzyme is tightly bound to the β subunit. Dialysis studies suggest that the two zinc ions differ in the strength of their association with the β subunit. The presence of zinc in β is consistent with several other lines of evidence which indicate that this subunit is directly involved in phosphodiester bond formation. The blue dextran-Sepharose column procedure should be useful in future studies of the dissociation and reassociation of the enzyme since the method is rapid and provides excellent recovery of the β subunit as well as the α and β′ subunits of the RNA polymerase.",
author = "Halling, {Shirley M.} and Sanchez-Anzaldo, {F. J.} and Ryuji Fukuda and Doi, {Roy H.} and Meares, {Claude F.}",
year = "1977",
language = "English (US)",
volume = "16",
pages = "2880--2884",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "13",

}

TY - JOUR

T1 - Zinc is associated with the β subunit of DNA-dependent RNA polymerase of Bacillus subtilis

AU - Halling, Shirley M.

AU - Sanchez-Anzaldo, F. J.

AU - Fukuda, Ryuji

AU - Doi, Roy H.

AU - Meares, Claude F.

PY - 1977

Y1 - 1977

N2 - The Bacillus subtilis DNA-dependent RNA polymerase holoenzyme and core enzyme each contain approximately two atoms of zinc per molecule. When the dissociated subunits of the enzyme are passed through a blue dextran-Sepharose affinity column, only the β subunit binds to the column. The total zinc content of the enzyme is tightly bound to the β subunit. Dialysis studies suggest that the two zinc ions differ in the strength of their association with the β subunit. The presence of zinc in β is consistent with several other lines of evidence which indicate that this subunit is directly involved in phosphodiester bond formation. The blue dextran-Sepharose column procedure should be useful in future studies of the dissociation and reassociation of the enzyme since the method is rapid and provides excellent recovery of the β subunit as well as the α and β′ subunits of the RNA polymerase.

AB - The Bacillus subtilis DNA-dependent RNA polymerase holoenzyme and core enzyme each contain approximately two atoms of zinc per molecule. When the dissociated subunits of the enzyme are passed through a blue dextran-Sepharose affinity column, only the β subunit binds to the column. The total zinc content of the enzyme is tightly bound to the β subunit. Dialysis studies suggest that the two zinc ions differ in the strength of their association with the β subunit. The presence of zinc in β is consistent with several other lines of evidence which indicate that this subunit is directly involved in phosphodiester bond formation. The blue dextran-Sepharose column procedure should be useful in future studies of the dissociation and reassociation of the enzyme since the method is rapid and provides excellent recovery of the β subunit as well as the α and β′ subunits of the RNA polymerase.

UR - http://www.scopus.com/inward/record.url?scp=0017698858&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017698858&partnerID=8YFLogxK

M3 - Article

C2 - 406910

AN - SCOPUS:0017698858

VL - 16

SP - 2880

EP - 2884

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 13

ER -