Zinc enhancement of nonviral salivary gland transfection

Edmund J. Niedzinski, David C. Olson, Yen Ju Chen, Judy A. Udove, Michael H. Nantz, Hsein C. Tseng, Janice L. Bolaffi, Michael J. Bennett

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Gene transfer to exocrine glands, including the major salivary glands, presents an attractive method to deliver proteins for therapeutic applications. Previous efforts using nonviral gene delivery to these glands have resulted in limited success. In this report, zinc and other divalent transitions were coadministered with plasmid DNA in an effort to improve nonviral salivary gland transfection efficiency. The inclusion of zinc into plasmid DNA solutions resulted in a 20-fold enhancement in transgene expression without noticeable inflammation compared to a solution of plasmid DNA only. This observed enhancement in transgene expression was dependent upon the DNA dose and correlated with the accumulation of plasmid DNA by salivary gland tissues.

Original languageEnglish (US)
Pages (from-to)396-400
Number of pages5
JournalMolecular Therapy
Volume7
Issue number3
DOIs
StatePublished - Mar 1 2003

Keywords

  • Exocrine glands
  • Gene transfer
  • Salivary glands
  • Transition metals
  • Zinc

ASJC Scopus subject areas

  • Molecular Biology

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    Niedzinski, E. J., Olson, D. C., Chen, Y. J., Udove, J. A., Nantz, M. H., Tseng, H. C., Bolaffi, J. L., & Bennett, M. J. (2003). Zinc enhancement of nonviral salivary gland transfection. Molecular Therapy, 7(3), 396-400. https://doi.org/10.1016/S1525-0016(02)00058-8