Zinc-deficient rat embryos have increased caspase 3-like activity and apoptosis

Margaret A. Jankowski-Hennig, Michael S. Clegg, George P. Daston, John M. Rogers, Carl L Keen

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Caspase activity is a hallmark of apoptosis. Given that maternal zinc (Zn) deficiency results in apoptosis in the rat embryo, we assessed caspase activity in Zn-deficient embryos. Mid-gestation rat embryos were collected from dams fed either a Zn-deficient (0.5 μg Zn/g) diet ad libitum, or a Zn-adequate (25 μg Zn/g) diet ad libitum or pair fed to dams fed the Zn-deficient diet. Embryos from dams fed the Zn-adequate diet had a normal level of cell death, while embryos from the dams fed the Zn-deficient diet had either increased or normal levels of cell death. Zn-deficient embryos displaying increased cell death had increased caspase activity. Embryos with normal levels of cell death, regardless of maternal diet, had similar caspase activities. Thus, Zn-deficiency-induced apoptosis in vivo is associated with increased caspase activity. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)250-256
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume271
Issue number1
DOIs
StatePublished - Apr 29 2000

Fingerprint

Caspase 3
Zinc
Rats
Embryonic Structures
Apoptosis
Nutrition
Caspases
Cell death
Diet
Dams
Cell Death
Mothers
Pregnancy

Keywords

  • Apoptosis
  • Caspase 3
  • Cell death
  • Deficiency
  • Development
  • Embryo
  • Pregnancy
  • Zinc

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Jankowski-Hennig, M. A., Clegg, M. S., Daston, G. P., Rogers, J. M., & Keen, C. L. (2000). Zinc-deficient rat embryos have increased caspase 3-like activity and apoptosis. Biochemical and Biophysical Research Communications, 271(1), 250-256. https://doi.org/10.1006/bbrc.2000.2608

Zinc-deficient rat embryos have increased caspase 3-like activity and apoptosis. / Jankowski-Hennig, Margaret A.; Clegg, Michael S.; Daston, George P.; Rogers, John M.; Keen, Carl L.

In: Biochemical and Biophysical Research Communications, Vol. 271, No. 1, 29.04.2000, p. 250-256.

Research output: Contribution to journalArticle

Jankowski-Hennig, Margaret A. ; Clegg, Michael S. ; Daston, George P. ; Rogers, John M. ; Keen, Carl L. / Zinc-deficient rat embryos have increased caspase 3-like activity and apoptosis. In: Biochemical and Biophysical Research Communications. 2000 ; Vol. 271, No. 1. pp. 250-256.
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