Zinc deficiency-induced anorexia influences the distribution of serum insulin-like growth factor-binding proteins in the rat

Michael S. Clegg, Carl L Keen, Sharon M. Donovan

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Zinc (Zn) deficiency can result in severe growth retardation in mammals, and in a number of animal model systems it leads to low circulating insulin-like growth factor-I (IGF-I) concentrations. Using a weanling male rat model and a number of feeding schemes, we show that in addition to lower circulating IGF-I concentrations, Zn deficiency leads to alterations in the distribution of serum IGF-binding proteins (IGFBPs). Serum from Zn-deficient animals labeled in vitro with [125I]IGF-I displayed three peaks of tracer activity: 150 kd (IGFBP-3), 37 kd (IGFBP-2 and -1), and 8 kd (free [125I]IGF-I). Relative to controls, Zn-deficient animals demonstrated more tracer binding in the 37-kd region, whereas less was found in the 150- and 8-kd peaks. Serum from chronically calorie-restricted fed animals displayed [125I]IGF-I binding profiles similar to Zn-deficient serum, implicating Zn deficiency-induced anorexia as the principle factor underlying both the lower circulating IGF-I and the alterations in IGFBP profiles. Concentrations of IGFBP-4 were unaffected by diet manipulation based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)/Western ligand blot (WLB) analysis.

Original languageEnglish (US)
Pages (from-to)1495-1501
Number of pages7
JournalMetabolism
Volume44
Issue number11
DOIs
StatePublished - 1995

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Insulin-Like Growth Factor Binding Proteins
Anorexia
Insulin-Like Growth Factor I
Zinc
Serum
Insulin-Like Growth Factor Binding Protein 4
Insulin-Like Growth Factor Binding Protein 2
Insulin-Like Growth Factor Binding Protein 1
Insulin-Like Growth Factor Binding Protein 3
Sodium Dodecyl Sulfate
Blood Proteins
Polyacrylamide Gel Electrophoresis
Mammals
Animal Models
Western Blotting
Diet
Ligands
Growth

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Zinc deficiency-induced anorexia influences the distribution of serum insulin-like growth factor-binding proteins in the rat. / Clegg, Michael S.; Keen, Carl L; Donovan, Sharon M.

In: Metabolism, Vol. 44, No. 11, 1995, p. 1495-1501.

Research output: Contribution to journalArticle

Clegg, Michael S. ; Keen, Carl L ; Donovan, Sharon M. / Zinc deficiency-induced anorexia influences the distribution of serum insulin-like growth factor-binding proteins in the rat. In: Metabolism. 1995 ; Vol. 44, No. 11. pp. 1495-1501.
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