Zinc deficiency in pregnant long-evans hooded rats: Teratogenicity and tissue trace elements

J. M. Rogers, Carl L Keen, L. S. Hurley

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The Long-Evans hooded rat is widely used in experimental teratology. This study determines the teratogenicity of maternal Zn deficiency in the Long-Evans hooded rat, and examines the effects of Zn deficiency on Zn, Fe, and Cu concentrations in maternal and fetal tissues. Dams were fed an egg white-based diet containing 100 μg/g Zn for 1 week prior to mating. At mating rats were fed diets with 0.5, 4.5, 9.0, or 100 μg/g Zn ad lib, or 100 μg/g pairfed to 0.5 μg/g Zn dams. Laparotomies were performed on day 21 of gestation. Live fetuses and resorptions were counted. Fetuses were weighed and examined for external malformations. Some fetuses were used for Zn, Fe, and Cu determinations, others for internal or skeletal examination. Zn, Fe, and Cu levels were determined in maternal liver, kidney, and plasma. The 0.5 μg/g Zn dams lost weight during pregnancy; 27% of implantation sites were resorbed, 91.7% of live fetuses were malformed, and fetal weight was low. There were no malformed fetuses in the 4.5 μg/g Zn or 9.0 μg/g Zn groups; litter weights were low in the 4.5 μg/g Zn group. Tissue Zn was correlated with dietary Zn. Increased Fe concentration occurred in all maternal and fetal tissues in the 0.5 μg/g Zn group. The teratogenicity of Zn deficiency in the Long-Evans rat appears similar to that previously reported in the Sprague-Dawley strain.

Original languageEnglish (US)
Pages (from-to)89-100
Number of pages12
JournalTeratology
Volume31
Issue number1
StatePublished - 1985

Fingerprint

Long Evans Rats
Trace Elements
Zinc
Rats
Fetus
Tissue
Dams
Nutrition
Mothers
Liver
Teratology
Diet
Weights and Measures
Pregnancy
Egg White
Fetal Weight
Plasmas
Laparotomy
Kidney

ASJC Scopus subject areas

  • Developmental Biology
  • Health, Toxicology and Mutagenesis
  • Embryology
  • Toxicology

Cite this

Rogers, J. M., Keen, C. L., & Hurley, L. S. (1985). Zinc deficiency in pregnant long-evans hooded rats: Teratogenicity and tissue trace elements. Teratology, 31(1), 89-100.

Zinc deficiency in pregnant long-evans hooded rats : Teratogenicity and tissue trace elements. / Rogers, J. M.; Keen, Carl L; Hurley, L. S.

In: Teratology, Vol. 31, No. 1, 1985, p. 89-100.

Research output: Contribution to journalArticle

Rogers, JM, Keen, CL & Hurley, LS 1985, 'Zinc deficiency in pregnant long-evans hooded rats: Teratogenicity and tissue trace elements', Teratology, vol. 31, no. 1, pp. 89-100.
Rogers, J. M. ; Keen, Carl L ; Hurley, L. S. / Zinc deficiency in pregnant long-evans hooded rats : Teratogenicity and tissue trace elements. In: Teratology. 1985 ; Vol. 31, No. 1. pp. 89-100.
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