Zinc deficiency causes oxidative damage to proteins, lipids and DNA in rat testes

P. I. Oteiza, K. L. Olin, C. G. Fraga, Carl L Keen

Research output: Contribution to journalArticle

248 Citations (Scopus)

Abstract

To investigate the effects of zinc deficiency on oxidative damage to testes proteins, lipids and DNA, weanling male rats were allowed free access to low (0.5 μg Zn/g) or adequate (25 μg Zn/g) zinc diets for 14 d. A third group was restricted intake of the adequate Zn diet to the amount consumed by the low Zn diet-fed group. Compared with ad libitum-fed controls, testes from rats fed the low zinc diet had lower glutamine synthetase activity, lower Fe2+-stimulated 2-thiobarbituric acid-reactive substances (TBARS) production, higher protein carbonyl concentrations (P < 0.05), and higher 8- oxo-2'-deoxyguanosine levels (P = 0.06). Glutamine synthetase activity in testes of the food-restricted controls was between the values for the ad libitum controls and zinc-deficient animals. Protein carbonyls were higher in the restricted controls compared with the ad libitum controls, whereas stimulated TBARS production was lower (P < 0.05). Levels of 8-oxo-2'- deoxyguanosine were lower in testes DNA of the restricted controls than in the zinc-deficient group (P < 0.05). Testes iron concentrations were higher in the zinc-deficient and restricted control rats than in ad libitum controls (P < 0.05). The oxidative damage observed may have occurred as a consequence of increased reactive oxygen species generation secondary to tissue iron accumulation and/or reductions in zinc-dependent antioxidant processes.

Original languageEnglish (US)
Pages (from-to)823-829
Number of pages7
JournalJournal of Nutrition
Volume125
Issue number4
StatePublished - 1995

Fingerprint

Testis
Zinc
testes
zinc
Lipids
DNA
rats
lipids
Proteins
Diet
proteins
Glutamate-Ammonia Ligase
Thiobarbituric Acid Reactive Substances
thiobarbituric acid
glutamate-ammonia ligase
iron
thiobarbituric acid-reactive substances
diet
Iron
Adequate Intakes

Keywords

  • lipid peroxidation
  • oxidative damage
  • rats
  • reactive oxygen species
  • zinc deficiency

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)

Cite this

Oteiza, P. I., Olin, K. L., Fraga, C. G., & Keen, C. L. (1995). Zinc deficiency causes oxidative damage to proteins, lipids and DNA in rat testes. Journal of Nutrition, 125(4), 823-829.

Zinc deficiency causes oxidative damage to proteins, lipids and DNA in rat testes. / Oteiza, P. I.; Olin, K. L.; Fraga, C. G.; Keen, Carl L.

In: Journal of Nutrition, Vol. 125, No. 4, 1995, p. 823-829.

Research output: Contribution to journalArticle

Oteiza, PI, Olin, KL, Fraga, CG & Keen, CL 1995, 'Zinc deficiency causes oxidative damage to proteins, lipids and DNA in rat testes', Journal of Nutrition, vol. 125, no. 4, pp. 823-829.
Oteiza, P. I. ; Olin, K. L. ; Fraga, C. G. ; Keen, Carl L. / Zinc deficiency causes oxidative damage to proteins, lipids and DNA in rat testes. In: Journal of Nutrition. 1995 ; Vol. 125, No. 4. pp. 823-829.
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