Zinc and copper in milk and tissues of nursing lethal milk mutant mice

J. E. Piletz, B. Lönnerdal, L. S. Hurley, W. Berry, R. E. Ganschow, H. R. Herschman

Research output: Contribution to journalArticle

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Abstract

Zinc concentration was lower in liver of suckling 1-d-old lethal milk (lm/lm) mutant mice than in wild-type pups, in accordance with the hypothesis of milk-induced zinc deficiency previously proposed to underlie this mutation. Despite the initial deficiency, by 3 d of age suckling lm/lm pups exhibited higher levels of hepatic zinc than did lm/lm-nursed wild-type pups. Intestinal zinc and copper concentrations were normal in 1-d-old lm/lm pups, but by 3 d of age were also higher in lm/lm pups than in wild-type pups foster-nursed on lm/lm dams. Contrary to a previous report, we found that zinc concentration in milk of lm/lm dams was not significantly different from those of controls, between 4-20 d postpartum. Mutant milk showed 1) normal zinc distribution as determined by gel-filtration chromatography or by DEAE-cellulose chromatography of zinc-binding ligands derived from EDTA-dissociated micelles, 2) normal copper levels, 3) normal amounts of citrate, a zinc (II) and copper (II)-binding ligand and 4) normal amounts of glutamate, a proposed copper (II)-binding ligand. Total mammary glands and mammary gland cytosols from lm/lm mice exhibited normal zinc concentrations. Copper levels, however, were higher in lm/lm mammary gland cytosols than in controls. These results suggest that an increased uptake and/or retention of zinc and copper in the tissues studied may underlie the signs of zinc deficiency seen in lethal milk mutant mice.

Original languageEnglish (US)
Pages (from-to)83-90
Number of pages8
JournalJournal of Nutrition
Volume117
Issue number1
StatePublished - 1987

Fingerprint

breast feeding
lethal genes
Zinc
Copper
Milk
Nursing
copper
zinc
milk
mutants
mice
pups
Human Mammary Glands
mammary glands
Cytosol
suckling
cytosol
tissues
Ligands
DEAE-Cellulose Chromatography

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Piletz, J. E., Lönnerdal, B., Hurley, L. S., Berry, W., Ganschow, R. E., & Herschman, H. R. (1987). Zinc and copper in milk and tissues of nursing lethal milk mutant mice. Journal of Nutrition, 117(1), 83-90.

Zinc and copper in milk and tissues of nursing lethal milk mutant mice. / Piletz, J. E.; Lönnerdal, B.; Hurley, L. S.; Berry, W.; Ganschow, R. E.; Herschman, H. R.

In: Journal of Nutrition, Vol. 117, No. 1, 1987, p. 83-90.

Research output: Contribution to journalArticle

Piletz, JE, Lönnerdal, B, Hurley, LS, Berry, W, Ganschow, RE & Herschman, HR 1987, 'Zinc and copper in milk and tissues of nursing lethal milk mutant mice', Journal of Nutrition, vol. 117, no. 1, pp. 83-90.
Piletz JE, Lönnerdal B, Hurley LS, Berry W, Ganschow RE, Herschman HR. Zinc and copper in milk and tissues of nursing lethal milk mutant mice. Journal of Nutrition. 1987;117(1):83-90.
Piletz, J. E. ; Lönnerdal, B. ; Hurley, L. S. ; Berry, W. ; Ganschow, R. E. ; Herschman, H. R. / Zinc and copper in milk and tissues of nursing lethal milk mutant mice. In: Journal of Nutrition. 1987 ; Vol. 117, No. 1. pp. 83-90.
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