Zinc and copper binding proteins in human milk

B. Lonnerdal, B. Hoffman, L. S. Hurley

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The proteins binding zinc and copper in human milk have been fractionated and identified, and the distribution of these trace elements among the different binding compounds has been determined. Casein was separated by ultracentrifugation and was found to contain 14% (range 5 to 21%) of the total zinc content in the milk and 28% (range 7 to 48%) of the total copper. Another zinc- and copper-binding protein was isolated by gel filtration and ion-exchange chromatograpy and identified as serum albumin by gel electrophoresis. Serum albumin in breast milk binds 28% of total zinc and 39% of total copper. The remainder of Zn and Cu was found to be in a low molecular weight form (29% (range 24 to 36%) of Zn; 24% (range 15 to 47%) or Cu) or associated to the fat (29% (range 20 to 45%) of Zn; 9% (range 1 to 21% of Cu). It is hypothesized that association constants of different binding compounds as well as their concentrations will determine the relative distribution of zinc and copper among them and may affect bioavailability of these elements for the infant.

Original languageEnglish (US)
Pages (from-to)1170-1176
Number of pages7
JournalAmerican Journal of Clinical Nutrition
Volume36
Issue number6
StatePublished - 1982

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Human Milk
breast milk
binding proteins
Zinc
Copper
copper
zinc
Serum Albumin
serum albumin
Ion Exchange
Ultracentrifugation
Trace Elements
Caseins
Biological Availability
Gel Chromatography
Electrophoresis
Milk
Molecular Weight
Gels
Fats

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)

Cite this

Lonnerdal, B., Hoffman, B., & Hurley, L. S. (1982). Zinc and copper binding proteins in human milk. American Journal of Clinical Nutrition, 36(6), 1170-1176.

Zinc and copper binding proteins in human milk. / Lonnerdal, B.; Hoffman, B.; Hurley, L. S.

In: American Journal of Clinical Nutrition, Vol. 36, No. 6, 1982, p. 1170-1176.

Research output: Contribution to journalArticle

Lonnerdal, B, Hoffman, B & Hurley, LS 1982, 'Zinc and copper binding proteins in human milk', American Journal of Clinical Nutrition, vol. 36, no. 6, pp. 1170-1176.
Lonnerdal B, Hoffman B, Hurley LS. Zinc and copper binding proteins in human milk. American Journal of Clinical Nutrition. 1982;36(6):1170-1176.
Lonnerdal, B. ; Hoffman, B. ; Hurley, L. S. / Zinc and copper binding proteins in human milk. In: American Journal of Clinical Nutrition. 1982 ; Vol. 36, No. 6. pp. 1170-1176.
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