Zebrafish fgf24 functions with fgf8 to promote posterior mesodermal development

Bruce W. Draper, David W. Stock, Charles B. Kimmel

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

Fibroblast growth factor (Fgf) signaling plays an important role during development of posterior mesoderm in vertebrate embryos. Blocking Fgf signaling by expressing a dominant-negative Fgf receptor inhibits posterior mesoderm development. In mice, Fgf8 appears to be the principal ligand required for mesodermal development, as mouse Fgf8 mutants do not form mesoderm. In zebrafish, Fgf8 is encoded by the acerebellar locus, and, similar to its mouse otholog, is expressed in early mesodermal precursors during gastrulation. However, zebrafish fgf8 mutants have only mild defects in posterior mesodermal development, suggesting that it is not the only Fgf ligand involved in the development of this tissue. We report here the identification of an fgf8-related gene in zebrafish, fgf24, that is co-expressed with fgf8 in mesodermal precursors during gastrulation. Using morpholino-based gene inactivation, we have analyzed the function of fgf24 during development. We found that inhibiting fgf24 function alone has no affect on the formation of posterior mesoderm. Conversely, inhibiting fgf24 function in embryos mutant for fgf8 blocks the formation of most posterior mesoderm. Thus, fgf8 and fgf24 are together required to promote posterior mesodermal development. We provide both phenotypic and genetic evidence that these Fgf signaling components interact with no tail and spadetail, two zebrafish T-box transcription factors that are required for the development of all posterior mesoderm. Last, we show that fgf24 is expressed in early fin bud mesenchyme and that inhibiting fgf24 function results in viable fish that lack pectoral fins.

Original languageEnglish (US)
Pages (from-to)4639-4654
Number of pages16
JournalDevelopment
Volume130
Issue number19
DOIs
StatePublished - Oct 2003
Externally publishedYes

Fingerprint

Mesoderm
Zebrafish
Fibroblast Growth Factors
Gastrulation
Embryonic Structures
Ligands
Fibroblast Growth Factor Receptors
Morpholinos
Gene Silencing
Vertebrates
Tail
Fishes
Transcription Factors
Genes

Keywords

  • Acerebellar
  • Fgf24
  • Fgf8
  • Fibroblast growth factor
  • Limb development
  • Mesoderm
  • No tail
  • Posterior development
  • Spadetail
  • Zebrafish

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Zebrafish fgf24 functions with fgf8 to promote posterior mesodermal development. / Draper, Bruce W.; Stock, David W.; Kimmel, Charles B.

In: Development, Vol. 130, No. 19, 10.2003, p. 4639-4654.

Research output: Contribution to journalArticle

Draper, Bruce W. ; Stock, David W. ; Kimmel, Charles B. / Zebrafish fgf24 functions with fgf8 to promote posterior mesodermal development. In: Development. 2003 ; Vol. 130, No. 19. pp. 4639-4654.
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