ZBTB33 binds unmethylated regions of the genome associated with actively expressed genes

Adam Blattler, Lijing Yao, Yao Wang, Zhenqing Ye, Victor X. Jin, Peggy J. Farnham

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Background: DNA methylation and repressive histone modifications cooperate to silence promoters. One mechanism by which regions of methylated DNA could acquire repressive histone modifications is via methyl DNA-binding transcription factors. The zinc finger protein ZBTB33 (also known as Kaiso) has been shown in vitro to bind preferentially to methylated DNA and to interact with the SMRT/NCoR histone deacetylase complexes. We have performed bioinformatic analyses of Kaiso ChIP-seq and DNA methylation datasets to test a model whereby binding of Kaiso to methylated CpGs leads to loss of acetylated histones at target promoters. Results: Our results suggest that, contrary to expectations, Kaiso does not bind to methylated DNA in vivo but instead binds to highly active promoters that are marked with high levels of acetylated histones. In addition, our studies suggest that DNA methylation and nucleosome occupancy patterns restrict access of Kaiso to potential binding sites and influence cell type-specific binding. Conclusions: We propose a new model for the genome-wide binding and function of Kaiso whereby Kaiso binds to unmethylated regulatory regions and contributes to the active state of target promoters.

Original languageEnglish (US)
Article number13
JournalEpigenetics and Chromatin
Volume6
Issue number1
DOIs
StatePublished - 2013
Externally publishedYes

Fingerprint

DNA Methylation
Histone Code
Genome
DNA
Histones
Genes
Histone Deacetylases
Nucleosomes
Nucleic Acid Regulatory Sequences
Zinc Fingers
Computational Biology
Transcription Factors
Binding Sites
Proteins

Keywords

  • DNA methylation
  • Epigenetics
  • Histone modifications
  • Transcription factor binding
  • Transcriptional regulation
  • Zinc finger proteins

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

ZBTB33 binds unmethylated regions of the genome associated with actively expressed genes. / Blattler, Adam; Yao, Lijing; Wang, Yao; Ye, Zhenqing; Jin, Victor X.; Farnham, Peggy J.

In: Epigenetics and Chromatin, Vol. 6, No. 1, 13, 2013.

Research output: Contribution to journalArticle

Blattler, Adam ; Yao, Lijing ; Wang, Yao ; Ye, Zhenqing ; Jin, Victor X. ; Farnham, Peggy J. / ZBTB33 binds unmethylated regions of the genome associated with actively expressed genes. In: Epigenetics and Chromatin. 2013 ; Vol. 6, No. 1.
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AU - Farnham, Peggy J.

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