ZBP1 subcellular localization and association with stress granules is controlled by its Z-DNA binding domains

Nikolaus Deigendesch, Friedrich Koch-Nolte, Stefan Rothenburg

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Z-DNA binding protein 1 (ZBP1) belongs to a family of proteins that contain the Zα domain, which binds specifically to left-handed Z-DNA and Z-RNA. Like all vertebrate proteins in the Zα family, it contains two Zα-like domains and is highly inducible by immunostimulation. Using circular dichroism spectroscopy and electrophoretic mobility shift assays we show that both Zα domains can bind Z-DNA independently and that substrate binding is greatly enhanced when both domains are linked. Full length ZBP1 and a prominent splice variant lacking the first Zα domain (ΔZα) showed strikingly different subcellular localizations. While the full length protein showed a finely punctate cytoplasmatic distribution, ZBP1ΔZα accumulated in large cytoplasmic granules. Mutation of residues important for Z-DNA binding in the first Zα domain resulted in a distribution comparable to that of ZBP1ΔZα. The ZBP1ΔZα granules are distinct from stress granules (SGs) and processing bodies but dynamically interacted with these. Polysome stabilization led to the disassembly of ZBP1ΔZα granules, indicating that mRNA are integral components. Heat shock and arsenite exposure had opposing effects on ZBP1 isoforms: while ZBP1ΔZα granules disassembled, full length ZBP1 accumulated in SGs. Our data link ZBP1 to mRNA sorting and metabolism and indicate distinct roles for ZBP1 isoforms.

Original languageEnglish (US)
Pages (from-to)5007-5020
Number of pages14
JournalNucleic Acids Research
Volume34
Issue number18
DOIs
StatePublished - Oct 15 2006
Externally publishedYes

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Z-Form DNA
DNA-Binding Proteins
Protein Isoforms
RNA Transport
Cytoplasmic Granules
Polyribosomes
Electrophoretic Mobility Shift Assay
Circular Dichroism
Vertebrates
Shock
Immunization
Spectrum Analysis
Hot Temperature

ASJC Scopus subject areas

  • Genetics

Cite this

ZBP1 subcellular localization and association with stress granules is controlled by its Z-DNA binding domains. / Deigendesch, Nikolaus; Koch-Nolte, Friedrich; Rothenburg, Stefan.

In: Nucleic Acids Research, Vol. 34, No. 18, 15.10.2006, p. 5007-5020.

Research output: Contribution to journalArticle

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