Yeast Mps1p Phosphorylates the Spindle Pole Component Spc110p in the N-terminal Domain

David B. Friedman, Joshua W. Kern, Brenda J. Huneycutt, Dani B.N. Vinh, Douglas K. Crawford, Estelle Steiner, David Scheiltz, John Yates, Katheryn A. Resing, Natalie G. Ahn, Mark Winey, Trisha N. Davis

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The yeast spindle pole body (SPB) component Spc110p (Nuf1p) undergoes specific serine/threonine phosphorylation as the mitotic spindle apparatus forms, and this phosphorylation persists until cells enter anaphase. We demonstrate that the dual-specificity kinase Mps1p is essential for the mitosis-specific phosphorylation of Spc110p in vivo and that Mps1p phosphorylates Spc110p in vitro. Phosphopeptides generated by proteolytic cleavage were identified and sequenced by mass spectrometry. Ser60, Thr64, and Thr68 are the major sites in Spc110p phosphorylated by Mps1p in vitro, and alanine substitution at these sites abolishes the mitosis-specific isoform in vivo. This is the first time that phosphorylation sites of an SPB component have been determined, and these are the first sites of Mps1p phosphorylation identified. Alanine substitution for any one of these phosphorylated residues, in conjunction with an alanine substitution at residue Ser36, is lethal in combination with alleles of SPC97, which encodes a component of the Tub4p complex. Consistent with a specific dysfunction for the alanine substitution mutations, simultaneous mutation of all four serine/threonine residues to aspartate does not confer any defect. Sites of Mps1p phosphorylation and Ser36 are located within the N-terminal globular domain of Spc110p, which resides at the inner plaque of the SPB and binds the Tub4p complex.

Original languageEnglish (US)
Pages (from-to)17958-17967
Number of pages10
JournalJournal of Biological Chemistry
Volume276
Issue number21
DOIs
StatePublished - Jan 25 2001
Externally publishedYes

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Spindle Poles
Phosphorylation
Yeast
Poles
Yeasts
Spindle Pole Bodies
Alanine
Substitution reactions
Threonine
Mitosis
Serine
Phosphopeptides
Anaphase
Mutation
Spindle Apparatus
Aspartic Acid
Mass spectrometry
Mass Spectrometry
Protein Isoforms
Phosphotransferases

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Friedman, D. B., Kern, J. W., Huneycutt, B. J., Vinh, D. B. N., Crawford, D. K., Steiner, E., ... Davis, T. N. (2001). Yeast Mps1p Phosphorylates the Spindle Pole Component Spc110p in the N-terminal Domain. Journal of Biological Chemistry, 276(21), 17958-17967. https://doi.org/10.1074/jbc.M010461200

Yeast Mps1p Phosphorylates the Spindle Pole Component Spc110p in the N-terminal Domain. / Friedman, David B.; Kern, Joshua W.; Huneycutt, Brenda J.; Vinh, Dani B.N.; Crawford, Douglas K.; Steiner, Estelle; Scheiltz, David; Yates, John; Resing, Katheryn A.; Ahn, Natalie G.; Winey, Mark; Davis, Trisha N.

In: Journal of Biological Chemistry, Vol. 276, No. 21, 25.01.2001, p. 17958-17967.

Research output: Contribution to journalArticle

Friedman, DB, Kern, JW, Huneycutt, BJ, Vinh, DBN, Crawford, DK, Steiner, E, Scheiltz, D, Yates, J, Resing, KA, Ahn, NG, Winey, M & Davis, TN 2001, 'Yeast Mps1p Phosphorylates the Spindle Pole Component Spc110p in the N-terminal Domain', Journal of Biological Chemistry, vol. 276, no. 21, pp. 17958-17967. https://doi.org/10.1074/jbc.M010461200
Friedman DB, Kern JW, Huneycutt BJ, Vinh DBN, Crawford DK, Steiner E et al. Yeast Mps1p Phosphorylates the Spindle Pole Component Spc110p in the N-terminal Domain. Journal of Biological Chemistry. 2001 Jan 25;276(21):17958-17967. https://doi.org/10.1074/jbc.M010461200
Friedman, David B. ; Kern, Joshua W. ; Huneycutt, Brenda J. ; Vinh, Dani B.N. ; Crawford, Douglas K. ; Steiner, Estelle ; Scheiltz, David ; Yates, John ; Resing, Katheryn A. ; Ahn, Natalie G. ; Winey, Mark ; Davis, Trisha N. / Yeast Mps1p Phosphorylates the Spindle Pole Component Spc110p in the N-terminal Domain. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 21. pp. 17958-17967.
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abstract = "The yeast spindle pole body (SPB) component Spc110p (Nuf1p) undergoes specific serine/threonine phosphorylation as the mitotic spindle apparatus forms, and this phosphorylation persists until cells enter anaphase. We demonstrate that the dual-specificity kinase Mps1p is essential for the mitosis-specific phosphorylation of Spc110p in vivo and that Mps1p phosphorylates Spc110p in vitro. Phosphopeptides generated by proteolytic cleavage were identified and sequenced by mass spectrometry. Ser60, Thr64, and Thr68 are the major sites in Spc110p phosphorylated by Mps1p in vitro, and alanine substitution at these sites abolishes the mitosis-specific isoform in vivo. This is the first time that phosphorylation sites of an SPB component have been determined, and these are the first sites of Mps1p phosphorylation identified. Alanine substitution for any one of these phosphorylated residues, in conjunction with an alanine substitution at residue Ser36, is lethal in combination with alleles of SPC97, which encodes a component of the Tub4p complex. Consistent with a specific dysfunction for the alanine substitution mutations, simultaneous mutation of all four serine/threonine residues to aspartate does not confer any defect. Sites of Mps1p phosphorylation and Ser36 are located within the N-terminal globular domain of Spc110p, which resides at the inner plaque of the SPB and binds the Tub4p complex.",
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