Yeast killer virus transcription initiation in vitro

Francis P. Barbone, Teresa L. Williams, Michael J Leibowitz

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Killer virions isolated from infected Saccharomyces cerevisiae cells contain an RNA polymerase activity which catalyzes the transcription in vitro of positive polarity RNAs from the L-A and M double-stranded RNA genomic segments of the virus. The RNA polymerase can initiate transcription in vitro with γ-thio-GTP, whose thiophosphate group is found on the 5′ terminus of transcripts. Transcripts produced in vitro by the virion-associated RNA polymerase in the presence of 7mGpppG are significantly more active as translational templates than are transcripts produced in its absence. However, unlike Escherichia coli RNA polymerase transcripts from viral cDNA made in the presence of 7mGpppG, transcripts produced by viral RNA polymerase in the presence of 7mGpppG fail to bind to antibody against 7mG.

Original languageEnglish (US)
Pages (from-to)333-337
Number of pages5
JournalVirology
Volume187
Issue number1
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

DNA-Directed RNA Polymerases
Yeasts
Viruses
Virion
Double-Stranded RNA
Viral RNA
Guanosine Triphosphate
Saccharomyces cerevisiae
Complementary DNA
In Vitro Techniques
RNA
Escherichia coli
Antibodies
7-methyl-diguanosine triphosphate

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Yeast killer virus transcription initiation in vitro. / Barbone, Francis P.; Williams, Teresa L.; Leibowitz, Michael J.

In: Virology, Vol. 187, No. 1, 1992, p. 333-337.

Research output: Contribution to journalArticle

Barbone, Francis P. ; Williams, Teresa L. ; Leibowitz, Michael J. / Yeast killer virus transcription initiation in vitro. In: Virology. 1992 ; Vol. 187, No. 1. pp. 333-337.
@article{69dda0b3e2534e03babd3af8b5122f5e,
title = "Yeast killer virus transcription initiation in vitro",
abstract = "Killer virions isolated from infected Saccharomyces cerevisiae cells contain an RNA polymerase activity which catalyzes the transcription in vitro of positive polarity RNAs from the L-A and M double-stranded RNA genomic segments of the virus. The RNA polymerase can initiate transcription in vitro with γ-thio-GTP, whose thiophosphate group is found on the 5′ terminus of transcripts. Transcripts produced in vitro by the virion-associated RNA polymerase in the presence of 7mGpppG are significantly more active as translational templates than are transcripts produced in its absence. However, unlike Escherichia coli RNA polymerase transcripts from viral cDNA made in the presence of 7mGpppG, transcripts produced by viral RNA polymerase in the presence of 7mGpppG fail to bind to antibody against 7mG.",
author = "Barbone, {Francis P.} and Williams, {Teresa L.} and Leibowitz, {Michael J}",
year = "1992",
doi = "10.1016/0042-6822(92)90323-H",
language = "English (US)",
volume = "187",
pages = "333--337",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Yeast killer virus transcription initiation in vitro

AU - Barbone, Francis P.

AU - Williams, Teresa L.

AU - Leibowitz, Michael J

PY - 1992

Y1 - 1992

N2 - Killer virions isolated from infected Saccharomyces cerevisiae cells contain an RNA polymerase activity which catalyzes the transcription in vitro of positive polarity RNAs from the L-A and M double-stranded RNA genomic segments of the virus. The RNA polymerase can initiate transcription in vitro with γ-thio-GTP, whose thiophosphate group is found on the 5′ terminus of transcripts. Transcripts produced in vitro by the virion-associated RNA polymerase in the presence of 7mGpppG are significantly more active as translational templates than are transcripts produced in its absence. However, unlike Escherichia coli RNA polymerase transcripts from viral cDNA made in the presence of 7mGpppG, transcripts produced by viral RNA polymerase in the presence of 7mGpppG fail to bind to antibody against 7mG.

AB - Killer virions isolated from infected Saccharomyces cerevisiae cells contain an RNA polymerase activity which catalyzes the transcription in vitro of positive polarity RNAs from the L-A and M double-stranded RNA genomic segments of the virus. The RNA polymerase can initiate transcription in vitro with γ-thio-GTP, whose thiophosphate group is found on the 5′ terminus of transcripts. Transcripts produced in vitro by the virion-associated RNA polymerase in the presence of 7mGpppG are significantly more active as translational templates than are transcripts produced in its absence. However, unlike Escherichia coli RNA polymerase transcripts from viral cDNA made in the presence of 7mGpppG, transcripts produced by viral RNA polymerase in the presence of 7mGpppG fail to bind to antibody against 7mG.

UR - http://www.scopus.com/inward/record.url?scp=0026597931&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026597931&partnerID=8YFLogxK

U2 - 10.1016/0042-6822(92)90323-H

DO - 10.1016/0042-6822(92)90323-H

M3 - Article

C2 - 1736538

AN - SCOPUS:0026597931

VL - 187

SP - 333

EP - 337

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -