Yeast β- and β'-coat proteins (COP). Two coatomer subunits essential for endoplasmic reticulum-to-Golgi protein traffic

R. Duden, M. Hosobuchi, S. Hamamoto, Mark Winey, B. Byers, R. Schekman

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

To understand better the role of non-clathrin coat proteins in membrane traffic, we have cloned and characterized two essential genes encoding subunits of the yeast coatomer, SEC26 and SEC27. Sec26p is a 109-kDa protein that shares 43% sequence identity with mammalian β-coat protein (β-COP). Sec26p-depleted cells accumulate endoplasmic reticulum (ER) forms of secretory precursor proteins, and growth ceases after a dramatic accumulation of ER membranes. Sec26p overproduction partially suppresses sec27-1, a new mutant that shows a temperature-sensitive defect in ER-to-Golgi transport. The SEC27 gene was cloned, and the sequence predicts a 99.4-kDa protein with 45% sequence identity to mammalian β'-COP. Our sequence data support a two- domain model for the SEC27 protein: a conserved amino-terminal domain, composed of five WD-40 repeats similar to those found in β-subunits of trimeric G proteins, and a less conserved carboxyl-terminal domain. Genetic interactions connect sec27-1 and sec21-1 (coatomer γ subunit) with the ARF1 and ARF2 genes and with the SEC22, BET1, and BOS1 genes, which encode membrane proteins involved in ER-to-Golgi transport.

Original languageEnglish (US)
Pages (from-to)24486-24495
Number of pages10
JournalJournal of Biological Chemistry
Volume269
Issue number39
StatePublished - Jan 1 1994
Externally publishedYes

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Fungal Proteins
Capsid Proteins
Endoplasmic Reticulum
Yeast
Genes
Membranes
Proteins
Gene encoding
Protein Precursors
GTP-Binding Proteins
Essential Genes
Membrane Proteins
Defects
Yeasts
Temperature
Growth

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Yeast β- and β'-coat proteins (COP). Two coatomer subunits essential for endoplasmic reticulum-to-Golgi protein traffic. / Duden, R.; Hosobuchi, M.; Hamamoto, S.; Winey, Mark; Byers, B.; Schekman, R.

In: Journal of Biological Chemistry, Vol. 269, No. 39, 01.01.1994, p. 24486-24495.

Research output: Contribution to journalArticle

Duden, R. ; Hosobuchi, M. ; Hamamoto, S. ; Winey, Mark ; Byers, B. ; Schekman, R. / Yeast β- and β'-coat proteins (COP). Two coatomer subunits essential for endoplasmic reticulum-to-Golgi protein traffic. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 39. pp. 24486-24495.
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