Y chromosome gene expression in the blood of male patients with ischemic stroke compared with male controls

Yingfang Tian, Boryana Stamova, Glen C. Jickling, Huichun Xu, Da Liu, Bradley Ander, Cheryl Bushnell, Xinhua Zhan, Renee J. Turner, Ryan R. Davis, Piero Verro, William C Pevec, Nasim Hedayati, David L Dawson, Jane Khoury, Edward C. Jauch, Arthur Pancioli, Joseph P. Broderick, Frank R Sharp

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Sex is suggested to be an important determinant of ischemic stroke risk factors, etiology, and outcome. However, the basis for this remains unclear. The Y chromosome is unique in males. Genes expressed in males on the Y chromosome that are associated with stroke may be important genetic contributors to the unique features of males with ischemic stroke, which would be helpful for explaining sex differences observed between men and women. Objective: We compared Y chromosome gene expression in males with ischemic stroke and male controls. Methods: Blood samples were obtained from 40 male patients ≤3, 5, and 24 hours after ischemic stroke and from 41 male controls (July 2003-April 2007). RNA was isolated from blood and was processed using Affymetrix Human U133 Plus 2.0 expression arrays (Affymetrix Inc., Santa Clara, California). Y chromosome genes differentially expressed between male patients with stroke and male control subjects were identified using an ANCOVA adjusted for age and batch. A P < 0.05 and a fold change >1.2 were considered significant. Results: Seven genes on the Y chromosome were differentially expressed in males with ischemic stroke compared with controls. Five of these genes (VAMP7, CSF2RA, SPRY3, DHRSX, and PLCXD1) are located on pseudoautosomal regions of the human Y chromosome. The other 2 genes (EIF1AY and DDX3Y) are located on the nonrecombining region of the human Y chromosome. The identified genes were associated with immunology, RNA metabolism, vesicle fusion, and angiogenesis. Conclusions: Specific genes on the Y chromosome are differentially expressed in blood after ischemic stroke. These genes provide insight into potential molecular contributors to sex differences in ischemic stroke.

Original languageEnglish (US)
JournalGender Medicine
Volume9
Issue number2
DOIs
StatePublished - Apr 2012

Fingerprint

Y-Linked Genes
stroke
Stroke
Gene Expression
Chromosomes, Human, Y
Genes
Y Chromosome
Sex Characteristics
RNA
etiology
Allergy and Immunology
determinants

Keywords

  • blood
  • gene expression
  • ischemic stroke
  • sex
  • Y chromosome

ASJC Scopus subject areas

  • Medicine(all)
  • Gender Studies

Cite this

Y chromosome gene expression in the blood of male patients with ischemic stroke compared with male controls. / Tian, Yingfang; Stamova, Boryana; Jickling, Glen C.; Xu, Huichun; Liu, Da; Ander, Bradley; Bushnell, Cheryl; Zhan, Xinhua; Turner, Renee J.; Davis, Ryan R.; Verro, Piero; Pevec, William C; Hedayati, Nasim; Dawson, David L; Khoury, Jane; Jauch, Edward C.; Pancioli, Arthur; Broderick, Joseph P.; Sharp, Frank R.

In: Gender Medicine, Vol. 9, No. 2, 04.2012.

Research output: Contribution to journalArticle

Tian, Yingfang ; Stamova, Boryana ; Jickling, Glen C. ; Xu, Huichun ; Liu, Da ; Ander, Bradley ; Bushnell, Cheryl ; Zhan, Xinhua ; Turner, Renee J. ; Davis, Ryan R. ; Verro, Piero ; Pevec, William C ; Hedayati, Nasim ; Dawson, David L ; Khoury, Jane ; Jauch, Edward C. ; Pancioli, Arthur ; Broderick, Joseph P. ; Sharp, Frank R. / Y chromosome gene expression in the blood of male patients with ischemic stroke compared with male controls. In: Gender Medicine. 2012 ; Vol. 9, No. 2.
@article{d820a273e3f6424b9f3216523d4efcf6,
title = "Y chromosome gene expression in the blood of male patients with ischemic stroke compared with male controls",
abstract = "Background: Sex is suggested to be an important determinant of ischemic stroke risk factors, etiology, and outcome. However, the basis for this remains unclear. The Y chromosome is unique in males. Genes expressed in males on the Y chromosome that are associated with stroke may be important genetic contributors to the unique features of males with ischemic stroke, which would be helpful for explaining sex differences observed between men and women. Objective: We compared Y chromosome gene expression in males with ischemic stroke and male controls. Methods: Blood samples were obtained from 40 male patients ≤3, 5, and 24 hours after ischemic stroke and from 41 male controls (July 2003-April 2007). RNA was isolated from blood and was processed using Affymetrix Human U133 Plus 2.0 expression arrays (Affymetrix Inc., Santa Clara, California). Y chromosome genes differentially expressed between male patients with stroke and male control subjects were identified using an ANCOVA adjusted for age and batch. A P < 0.05 and a fold change >1.2 were considered significant. Results: Seven genes on the Y chromosome were differentially expressed in males with ischemic stroke compared with controls. Five of these genes (VAMP7, CSF2RA, SPRY3, DHRSX, and PLCXD1) are located on pseudoautosomal regions of the human Y chromosome. The other 2 genes (EIF1AY and DDX3Y) are located on the nonrecombining region of the human Y chromosome. The identified genes were associated with immunology, RNA metabolism, vesicle fusion, and angiogenesis. Conclusions: Specific genes on the Y chromosome are differentially expressed in blood after ischemic stroke. These genes provide insight into potential molecular contributors to sex differences in ischemic stroke.",
keywords = "blood, gene expression, ischemic stroke, sex, Y chromosome",
author = "Yingfang Tian and Boryana Stamova and Jickling, {Glen C.} and Huichun Xu and Da Liu and Bradley Ander and Cheryl Bushnell and Xinhua Zhan and Turner, {Renee J.} and Davis, {Ryan R.} and Piero Verro and Pevec, {William C} and Nasim Hedayati and Dawson, {David L} and Jane Khoury and Jauch, {Edward C.} and Arthur Pancioli and Broderick, {Joseph P.} and Sharp, {Frank R}",
year = "2012",
month = "4",
doi = "10.1016/j.genm.2012.01.005",
language = "English (US)",
volume = "9",
journal = "Gender Medicine",
issn = "1550-8579",
publisher = "Excerpta Medica",
number = "2",

}

TY - JOUR

T1 - Y chromosome gene expression in the blood of male patients with ischemic stroke compared with male controls

AU - Tian, Yingfang

AU - Stamova, Boryana

AU - Jickling, Glen C.

AU - Xu, Huichun

AU - Liu, Da

AU - Ander, Bradley

AU - Bushnell, Cheryl

AU - Zhan, Xinhua

AU - Turner, Renee J.

AU - Davis, Ryan R.

AU - Verro, Piero

AU - Pevec, William C

AU - Hedayati, Nasim

AU - Dawson, David L

AU - Khoury, Jane

AU - Jauch, Edward C.

AU - Pancioli, Arthur

AU - Broderick, Joseph P.

AU - Sharp, Frank R

PY - 2012/4

Y1 - 2012/4

N2 - Background: Sex is suggested to be an important determinant of ischemic stroke risk factors, etiology, and outcome. However, the basis for this remains unclear. The Y chromosome is unique in males. Genes expressed in males on the Y chromosome that are associated with stroke may be important genetic contributors to the unique features of males with ischemic stroke, which would be helpful for explaining sex differences observed between men and women. Objective: We compared Y chromosome gene expression in males with ischemic stroke and male controls. Methods: Blood samples were obtained from 40 male patients ≤3, 5, and 24 hours after ischemic stroke and from 41 male controls (July 2003-April 2007). RNA was isolated from blood and was processed using Affymetrix Human U133 Plus 2.0 expression arrays (Affymetrix Inc., Santa Clara, California). Y chromosome genes differentially expressed between male patients with stroke and male control subjects were identified using an ANCOVA adjusted for age and batch. A P < 0.05 and a fold change >1.2 were considered significant. Results: Seven genes on the Y chromosome were differentially expressed in males with ischemic stroke compared with controls. Five of these genes (VAMP7, CSF2RA, SPRY3, DHRSX, and PLCXD1) are located on pseudoautosomal regions of the human Y chromosome. The other 2 genes (EIF1AY and DDX3Y) are located on the nonrecombining region of the human Y chromosome. The identified genes were associated with immunology, RNA metabolism, vesicle fusion, and angiogenesis. Conclusions: Specific genes on the Y chromosome are differentially expressed in blood after ischemic stroke. These genes provide insight into potential molecular contributors to sex differences in ischemic stroke.

AB - Background: Sex is suggested to be an important determinant of ischemic stroke risk factors, etiology, and outcome. However, the basis for this remains unclear. The Y chromosome is unique in males. Genes expressed in males on the Y chromosome that are associated with stroke may be important genetic contributors to the unique features of males with ischemic stroke, which would be helpful for explaining sex differences observed between men and women. Objective: We compared Y chromosome gene expression in males with ischemic stroke and male controls. Methods: Blood samples were obtained from 40 male patients ≤3, 5, and 24 hours after ischemic stroke and from 41 male controls (July 2003-April 2007). RNA was isolated from blood and was processed using Affymetrix Human U133 Plus 2.0 expression arrays (Affymetrix Inc., Santa Clara, California). Y chromosome genes differentially expressed between male patients with stroke and male control subjects were identified using an ANCOVA adjusted for age and batch. A P < 0.05 and a fold change >1.2 were considered significant. Results: Seven genes on the Y chromosome were differentially expressed in males with ischemic stroke compared with controls. Five of these genes (VAMP7, CSF2RA, SPRY3, DHRSX, and PLCXD1) are located on pseudoautosomal regions of the human Y chromosome. The other 2 genes (EIF1AY and DDX3Y) are located on the nonrecombining region of the human Y chromosome. The identified genes were associated with immunology, RNA metabolism, vesicle fusion, and angiogenesis. Conclusions: Specific genes on the Y chromosome are differentially expressed in blood after ischemic stroke. These genes provide insight into potential molecular contributors to sex differences in ischemic stroke.

KW - blood

KW - gene expression

KW - ischemic stroke

KW - sex

KW - Y chromosome

UR - http://www.scopus.com/inward/record.url?scp=84862817221&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862817221&partnerID=8YFLogxK

U2 - 10.1016/j.genm.2012.01.005

DO - 10.1016/j.genm.2012.01.005

M3 - Article

C2 - 22365286

AN - SCOPUS:84862817221

VL - 9

JO - Gender Medicine

JF - Gender Medicine

SN - 1550-8579

IS - 2

ER -