XRCC3 promotes homology-directed repair of DNA damage in mammalian cells

Andrew J. Pierce, Roger D. Johnson, Larry H. Thompson, Maria Jasin

Research output: Contribution to journalArticle

867 Scopus citations

Abstract

Homology-directed repair of DNA damage has recently emerged as a major mechanism for the maintenance of genomic integrity in mammalian cells. The highly conserved strand transferase, Rad51, is expected to be critical for this process. XRCC3 possesses a limited sequence similarity to Rad51 and interacts with it. Using a novel fluorescence-based assay, we demonstrate here that error-free homology-directed repair of DNA double-strand breaks is decreased 25-fold in an XRCC3-deficient hamster cell line and can be restored to wild-type levels through XRCC3 expression. These results establish that XRCC3-mediated homologous recombination can reverse DNA damage that would otherwise be mutagenic or lethal.

Original languageEnglish (US)
Pages (from-to)2633-2638
Number of pages6
JournalGenes and Development
Volume13
Issue number20
DOIs
StatePublished - Oct 15 1999
Externally publishedYes

Keywords

  • Double-strand break repair
  • Gene conversion
  • Homologous recombination
  • Mammalian cells
  • Rad51-related proteins
  • XRCC3

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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  • Cite this

    Pierce, A. J., Johnson, R. D., Thompson, L. H., & Jasin, M. (1999). XRCC3 promotes homology-directed repair of DNA damage in mammalian cells. Genes and Development, 13(20), 2633-2638. https://doi.org/10.1101/gad.13.20.2633