XRCC3 and Rad51 modulate replication fork progression on damaged vertebrate chromosomes

Judith Henry-Mowatt, Dean Jackson, Jean Yves Masson, Penny A. Johnson, Paula M. Clements, Fiona E. Benson, Larry H. Thompson, Shunichi Takeda, Stephen C. West, Keith W. Caldecott

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

The mechanisms by which the progression of eukaryotic replication forks is controlled after DNA damage are unclear. We have found that fork progression is slowed by cisplatin or UV treatment in intact vertebrate cells and in replication assays in vitro. Fork slowing is reduced or absent in irs1SF CHO cells and XRCC3-/- chicken DT40 cells, indicating that fork slowing is an active process that requires the homologous recombination protein XRCC3. The addition of purified human Rad51C-XRCC3 complex restores fork slowing in permeabilized XRCC3-/- cells. Moreover, the requirement for XRCC3 for fork slowing can be circumvented by addition of human Rad51. These data demonstrate that the recombination proteins XRCC3 and Rad51 cooperatively modulate the progression of replication forks on damaged vertebrate chromosomes.

Original languageEnglish (US)
Pages (from-to)1109-1117
Number of pages9
JournalMolecular Cell
Volume11
Issue number4
DOIs
StatePublished - Apr 1 2003
Externally publishedYes

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Vertebrates
Chromosomes
CHO Cells
Homologous Recombination
Cisplatin
Genetic Recombination
DNA Damage
Chickens
X-ray repair cross complementing protein 3
In Vitro Techniques

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Henry-Mowatt, J., Jackson, D., Masson, J. Y., Johnson, P. A., Clements, P. M., Benson, F. E., ... Caldecott, K. W. (2003). XRCC3 and Rad51 modulate replication fork progression on damaged vertebrate chromosomes. Molecular Cell, 11(4), 1109-1117. https://doi.org/10.1016/S1097-2765(03)00132-1

XRCC3 and Rad51 modulate replication fork progression on damaged vertebrate chromosomes. / Henry-Mowatt, Judith; Jackson, Dean; Masson, Jean Yves; Johnson, Penny A.; Clements, Paula M.; Benson, Fiona E.; Thompson, Larry H.; Takeda, Shunichi; West, Stephen C.; Caldecott, Keith W.

In: Molecular Cell, Vol. 11, No. 4, 01.04.2003, p. 1109-1117.

Research output: Contribution to journalArticle

Henry-Mowatt, J, Jackson, D, Masson, JY, Johnson, PA, Clements, PM, Benson, FE, Thompson, LH, Takeda, S, West, SC & Caldecott, KW 2003, 'XRCC3 and Rad51 modulate replication fork progression on damaged vertebrate chromosomes', Molecular Cell, vol. 11, no. 4, pp. 1109-1117. https://doi.org/10.1016/S1097-2765(03)00132-1
Henry-Mowatt J, Jackson D, Masson JY, Johnson PA, Clements PM, Benson FE et al. XRCC3 and Rad51 modulate replication fork progression on damaged vertebrate chromosomes. Molecular Cell. 2003 Apr 1;11(4):1109-1117. https://doi.org/10.1016/S1097-2765(03)00132-1
Henry-Mowatt, Judith ; Jackson, Dean ; Masson, Jean Yves ; Johnson, Penny A. ; Clements, Paula M. ; Benson, Fiona E. ; Thompson, Larry H. ; Takeda, Shunichi ; West, Stephen C. ; Caldecott, Keith W. / XRCC3 and Rad51 modulate replication fork progression on damaged vertebrate chromosomes. In: Molecular Cell. 2003 ; Vol. 11, No. 4. pp. 1109-1117.
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