XMAP215 Is a Processive Microtubule Polymerase

Gary J. Brouhard, Jeffrey H. Stear, Tim L. Noetzel, Jawdat Al-Bassam, Kazuhisa Kinoshita, Stephen C. Harrison, Jonathon Howard, Anthony A. Hyman

Research output: Contribution to journalArticle

304 Citations (Scopus)

Abstract

Fast growth of microtubules is essential for rapid assembly of the microtubule cytoskeleton during cell proliferation and differentiation. XMAP215 belongs to a conserved family of proteins that promote microtubule growth. To determine how XMAP215 accelerates growth, we developed a single-molecule assay to visualize directly XMAP215-GFP interacting with dynamic microtubules. XMAP215 binds free tubulin in a 1:1 complex that interacts with the microtubule lattice and targets the ends by a diffusion-facilitated mechanism. XMAP215 persists at the plus end for many rounds of tubulin subunit addition in a form of "tip tracking." These results show that XMAP215 is a processive polymerase that directly catalyzes the addition of up to 25 tubulin dimers to the growing plus end. Under some circumstances XMAP215 can also catalyze the reverse reaction, namely microtubule shrinkage. The similarities between XMAP215 and formins, actin polymerases, suggest that processive tip tracking is a common mechanism for stimulating the growth of cytoskeletal polymers.

Original languageEnglish (US)
Pages (from-to)79-88
Number of pages10
JournalCell
Volume132
Issue number1
DOIs
StatePublished - Jan 11 2008
Externally publishedYes

Fingerprint

Microtubules
Tubulin
Growth
Microtubule Proteins
Facilitated Diffusion
Cell proliferation
Dimers
Actins
Assays
Polymers
Cytoskeleton
Cell Differentiation
Molecules
Cell Proliferation

Keywords

  • CELLBIO

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Brouhard, G. J., Stear, J. H., Noetzel, T. L., Al-Bassam, J., Kinoshita, K., Harrison, S. C., ... Hyman, A. A. (2008). XMAP215 Is a Processive Microtubule Polymerase. Cell, 132(1), 79-88. https://doi.org/10.1016/j.cell.2007.11.043

XMAP215 Is a Processive Microtubule Polymerase. / Brouhard, Gary J.; Stear, Jeffrey H.; Noetzel, Tim L.; Al-Bassam, Jawdat; Kinoshita, Kazuhisa; Harrison, Stephen C.; Howard, Jonathon; Hyman, Anthony A.

In: Cell, Vol. 132, No. 1, 11.01.2008, p. 79-88.

Research output: Contribution to journalArticle

Brouhard, GJ, Stear, JH, Noetzel, TL, Al-Bassam, J, Kinoshita, K, Harrison, SC, Howard, J & Hyman, AA 2008, 'XMAP215 Is a Processive Microtubule Polymerase', Cell, vol. 132, no. 1, pp. 79-88. https://doi.org/10.1016/j.cell.2007.11.043
Brouhard GJ, Stear JH, Noetzel TL, Al-Bassam J, Kinoshita K, Harrison SC et al. XMAP215 Is a Processive Microtubule Polymerase. Cell. 2008 Jan 11;132(1):79-88. https://doi.org/10.1016/j.cell.2007.11.043
Brouhard, Gary J. ; Stear, Jeffrey H. ; Noetzel, Tim L. ; Al-Bassam, Jawdat ; Kinoshita, Kazuhisa ; Harrison, Stephen C. ; Howard, Jonathon ; Hyman, Anthony A. / XMAP215 Is a Processive Microtubule Polymerase. In: Cell. 2008 ; Vol. 132, No. 1. pp. 79-88.
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