TY - JOUR
T1 - Wteredinibacter haidensis sp. Nov., teredinibacter purpureus sp. nov. and teredinibacter franksiae sp. nov., marine, cellulolytic endosymbiotic bacteria isolated from the gills of the wood-boring mollusc bankia setacea (bivalvia
T2 - Teredinidae) and emended description of the genus teredinibacter
AU - Altamia, Marvin A.
AU - Reuben Shipway, J.
AU - Stein, David
AU - Betcher, Meghan A.
AU - Fung, Jennifer M.
AU - Jospin, Guillaume
AU - Eisen, Jonathan
AU - Haygood, Margo G.
AU - Distel, Daniel L.
N1 - Funding Information:
Research reported in this publication was supported by the Fogarty International CenterCentre of the National Institutes of Health under Award Number U19TW008163 (to M.G.H and D.L.D.), National Science Foundation awards IOS 1442759 (to D.L.D.) and DBI 1722553 and National Oceanic and Atmospheric Administration award NA19OAR010303 (to D.L.D. and M.G.H.). DNA sequence data used in this investigation were produced by the US Department of Energy Joint Genome Institute through a grant from the Community Sequencing Program, JGI User Agreement No. FP00002075 and by New England Biolabs, Inc. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health or the National Science Foundation. The authors wish to thank Donald G. Comb, Richard J. Roberts, and Alexey Fomenkov (New England Biolabs) for support of and assistance with genome sequencing efforts and to the students and faculty of the Hydaburg City School District and members of the Haida Nation, Hydaburg, Alaska for their enthusiastic contributions to this research.
Funding Information:
Research reported in this publication was supported by the Fogarty International CenterCentre of the National Institutes of Health under Award Number U19TW008163 (to M.G.H and D.L.D.), National Science Foundation awards IOS 1442759 (to D.L.D.) and DBI 1722553 and National Oceanic and Atmospheric Administration award NA19OAR010303 (to D.L.D. and M.G.H.). DNA sequence data used in this investigation were produced by the US Department of Energy Joint Genome Institute through a grant from the Community Sequencing Program, JGI User Agreement No. FP00002075 and by New England Biolabs, Inc. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health or the National Science Foundation.
Publisher Copyright:
© 2021 The Authors.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021
Y1 - 2021
N2 - Here, we describe three endosymbiotic bacterial strains isolated from the gills of the shipworm, Bankia setacea (Teredinidae: Bivalvia). These strains, designated as Bs08T, Bs12T and Bsc2T, are Gram-stain-negative, microaerobic, gammaproteobacteria that grow on cellulose and a variety of substrates derived from lignocellulose. Phenotypic characterization, phylogeny based on 16S rRNA gene and whole genome sequence data, amino acid identity and percentage of conserved proteins analyses, show that these strains are novel and may be assigned to the genus Teredinibacter. The three strains may be differentiated and dis-tinguished from other previously described Teredinibacter species based on a combination of four characteristics: colony colour (Bs12T, purple; others beige to brown), marine salt requirement (Bs12T, Bsc2T and Teredinibacter turnerae strains), the capacity for nitrogen fixation (Bs08T and T. turnerae strains) and the ability to respire nitrate (Bs08T). Based on these findings, we propose the names Teredinibacter haidensis sp. nov. (type strain Bs08T=ATCC TSD-121T=KCTC 62964T), Teredinibacter purpureus sp. nov. (type strain Bs12T=ATCC TSD-122T=KCTC 62965T) and Teredinibacter franksiae sp. nov. (type strain Bsc2T=ATCC TSD-123T=KCTC 62966T).
AB - Here, we describe three endosymbiotic bacterial strains isolated from the gills of the shipworm, Bankia setacea (Teredinidae: Bivalvia). These strains, designated as Bs08T, Bs12T and Bsc2T, are Gram-stain-negative, microaerobic, gammaproteobacteria that grow on cellulose and a variety of substrates derived from lignocellulose. Phenotypic characterization, phylogeny based on 16S rRNA gene and whole genome sequence data, amino acid identity and percentage of conserved proteins analyses, show that these strains are novel and may be assigned to the genus Teredinibacter. The three strains may be differentiated and dis-tinguished from other previously described Teredinibacter species based on a combination of four characteristics: colony colour (Bs12T, purple; others beige to brown), marine salt requirement (Bs12T, Bsc2T and Teredinibacter turnerae strains), the capacity for nitrogen fixation (Bs08T and T. turnerae strains) and the ability to respire nitrate (Bs08T). Based on these findings, we propose the names Teredinibacter haidensis sp. nov. (type strain Bs08T=ATCC TSD-121T=KCTC 62964T), Teredinibacter purpureus sp. nov. (type strain Bs12T=ATCC TSD-122T=KCTC 62965T) and Teredinibacter franksiae sp. nov. (type strain Bsc2T=ATCC TSD-123T=KCTC 62966T).
KW - Conjugative elements
KW - Horizontal gene transfer
KW - Lateral gene transfer
KW - Lignocellulose degradation
KW - Nitrate respiration
KW - Nitrogen fixation
KW - Plasmid
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U2 - 10.1099/ijsem.0.004627
DO - 10.1099/ijsem.0.004627
M3 - Article
C2 - 33439117
AN - SCOPUS:85101512573
VL - 71
SP - 1
EP - 11
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
SN - 1466-5026
IS - 2
M1 - 004627
ER -