Wrch-1, a novel member of the Rho gene family that is regulated by Wnt-1

Weikang Tao, Diane Pennica, Lifeng Xu, Robert F. Kalejta, Arnold J. Levine

Research output: Contribution to journalArticle

167 Scopus citations

Abstract

We report the isolation and cloning of the Wrch-1 (Wnt-1 responsive Cdc42 homolog) cDNA. Wrch-1 is a novel gene whose mRNA level increases in response to Wnt-1 signaling in Wnt-1 transformed cells, Wnt-1 transgene induced mouse mammary tumors, and Wnt-1 retrovirus infected cells. Wrch-1 encodes a homolog of the Rho family of GTPases. It shares 57% amino acid sequence identity with Cdc42, but possesses a unique N-terminal domain that contains several putative PXXP SH3-binding motifs. Like Cdc42, Wrch-1 can activate PAK-1 and JNK-1, and induce filopodium formation and stress fiber dissolution. Active Wrch-1 stimulates quiescent cells to reenter the cell cycle. Moreover, overexpression of Wrch-1 phenocopies Wnt-1 in morphological transformation of mouse mammary epithelial cells. Taken together, Wrch-1 could mediate the effects of Wnt-1 signaling in the regulation of cell morphology, cytoskeletal organization, and cell proliferation.

Original languageEnglish (US)
Pages (from-to)1796-1807
Number of pages12
JournalGenes and Development
Volume15
Issue number14
DOIs
StatePublished - Jul 15 2001
Externally publishedYes

Keywords

  • β-catenin
  • Cdc42
  • GTPase
  • Rho
  • Wnt-1
  • Wrch-1

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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  • Cite this

    Tao, W., Pennica, D., Xu, L., Kalejta, R. F., & Levine, A. J. (2001). Wrch-1, a novel member of the Rho gene family that is regulated by Wnt-1. Genes and Development, 15(14), 1796-1807. https://doi.org/10.1101/gad.894301