Abstract
We report the isolation and cloning of the Wrch-1 (Wnt-1 responsive Cdc42 homolog) cDNA. Wrch-1 is a novel gene whose mRNA level increases in response to Wnt-1 signaling in Wnt-1 transformed cells, Wnt-1 transgene induced mouse mammary tumors, and Wnt-1 retrovirus infected cells. Wrch-1 encodes a homolog of the Rho family of GTPases. It shares 57% amino acid sequence identity with Cdc42, but possesses a unique N-terminal domain that contains several putative PXXP SH3-binding motifs. Like Cdc42, Wrch-1 can activate PAK-1 and JNK-1, and induce filopodium formation and stress fiber dissolution. Active Wrch-1 stimulates quiescent cells to reenter the cell cycle. Moreover, overexpression of Wrch-1 phenocopies Wnt-1 in morphological transformation of mouse mammary epithelial cells. Taken together, Wrch-1 could mediate the effects of Wnt-1 signaling in the regulation of cell morphology, cytoskeletal organization, and cell proliferation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1796-1807 |
| Number of pages | 12 |
| Journal | Genes and Development |
| Volume | 15 |
| Issue number | 14 |
| DOIs | |
| State | Published - Jul 15 2001 |
| Externally published | Yes |
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Keywords
- β-catenin
- Cdc42
- GTPase
- Rho
- Wnt-1
- Wrch-1
ASJC Scopus subject areas
- Genetics
- Developmental Biology
Cite this
Wrch-1, a novel member of the Rho gene family that is regulated by Wnt-1. / Tao, Weikang; Pennica, Diane; Xu, Lifeng; Kalejta, Robert F.; Levine, Arnold J.
In: Genes and Development, Vol. 15, No. 14, 15.07.2001, p. 1796-1807.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Wrch-1, a novel member of the Rho gene family that is regulated by Wnt-1
AU - Tao, Weikang
AU - Pennica, Diane
AU - Xu, Lifeng
AU - Kalejta, Robert F.
AU - Levine, Arnold J.
PY - 2001/7/15
Y1 - 2001/7/15
N2 - We report the isolation and cloning of the Wrch-1 (Wnt-1 responsive Cdc42 homolog) cDNA. Wrch-1 is a novel gene whose mRNA level increases in response to Wnt-1 signaling in Wnt-1 transformed cells, Wnt-1 transgene induced mouse mammary tumors, and Wnt-1 retrovirus infected cells. Wrch-1 encodes a homolog of the Rho family of GTPases. It shares 57% amino acid sequence identity with Cdc42, but possesses a unique N-terminal domain that contains several putative PXXP SH3-binding motifs. Like Cdc42, Wrch-1 can activate PAK-1 and JNK-1, and induce filopodium formation and stress fiber dissolution. Active Wrch-1 stimulates quiescent cells to reenter the cell cycle. Moreover, overexpression of Wrch-1 phenocopies Wnt-1 in morphological transformation of mouse mammary epithelial cells. Taken together, Wrch-1 could mediate the effects of Wnt-1 signaling in the regulation of cell morphology, cytoskeletal organization, and cell proliferation.
AB - We report the isolation and cloning of the Wrch-1 (Wnt-1 responsive Cdc42 homolog) cDNA. Wrch-1 is a novel gene whose mRNA level increases in response to Wnt-1 signaling in Wnt-1 transformed cells, Wnt-1 transgene induced mouse mammary tumors, and Wnt-1 retrovirus infected cells. Wrch-1 encodes a homolog of the Rho family of GTPases. It shares 57% amino acid sequence identity with Cdc42, but possesses a unique N-terminal domain that contains several putative PXXP SH3-binding motifs. Like Cdc42, Wrch-1 can activate PAK-1 and JNK-1, and induce filopodium formation and stress fiber dissolution. Active Wrch-1 stimulates quiescent cells to reenter the cell cycle. Moreover, overexpression of Wrch-1 phenocopies Wnt-1 in morphological transformation of mouse mammary epithelial cells. Taken together, Wrch-1 could mediate the effects of Wnt-1 signaling in the regulation of cell morphology, cytoskeletal organization, and cell proliferation.
KW - β-catenin
KW - Cdc42
KW - GTPase
KW - Rho
KW - Wnt-1
KW - Wrch-1
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UR - http://www.scopus.com/inward/citedby.url?scp=0035878110&partnerID=8YFLogxK
U2 - 10.1101/gad.894301
DO - 10.1101/gad.894301
M3 - Article
C2 - 11459829
AN - SCOPUS:0035878110
VL - 15
SP - 1796
EP - 1807
JO - Genes and Development
JF - Genes and Development
SN - 0890-9369
IS - 14
ER -