Abstract
Cultured keratinocyte grafts transplanted for skin wound repair are often affixed to a wound dressing to facilitate handling. In this study, the ability of five different types of wound dressings to support cell viability and maintain stem cell populations in the cultured grafts was determined. Postconfluent keratinocyte (NHK) sheets were attached to wound dressings for 24 h and then released by trypsinization. Cell viability was determined and NHKs were assessed for clonogenic capacity by colony-forming efficiency (CFE) assays. CFEs for NHKs exposed to a collagen-bonded, bilaminate membrane and a polyurethane film were significantly less than control. On the other hand, CFEs for NHKs exposed to a collagen/alginate dressing and to petrolatum-impregnated gauze were significantly greater than control. The choice of a wound dressing carrier has implications for maintaining long-term viability of the transplanted sheet of epithelium.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 749-754 |
| Number of pages | 6 |
| Journal | Cell Transplantation |
| Volume | 10 |
| Issue number | 8 |
| State | Published - 2001 |
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Keywords
- Biological dressings
- Colony-forming units assay
- Keratinocyte transplantation
- Stem cell
ASJC Scopus subject areas
- Cell Biology
- Transplantation
Cite this
Wound dressings alter the colony-forming efficiency of keratinocytes in cultured sheet grafts. / DeGraffenried, Lea Ann; Isseroff, Roslyn Rivkah.
In: Cell Transplantation, Vol. 10, No. 8, 2001, p. 749-754.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Wound dressings alter the colony-forming efficiency of keratinocytes in cultured sheet grafts
AU - DeGraffenried, Lea Ann
AU - Isseroff, Roslyn Rivkah
PY - 2001
Y1 - 2001
N2 - Cultured keratinocyte grafts transplanted for skin wound repair are often affixed to a wound dressing to facilitate handling. In this study, the ability of five different types of wound dressings to support cell viability and maintain stem cell populations in the cultured grafts was determined. Postconfluent keratinocyte (NHK) sheets were attached to wound dressings for 24 h and then released by trypsinization. Cell viability was determined and NHKs were assessed for clonogenic capacity by colony-forming efficiency (CFE) assays. CFEs for NHKs exposed to a collagen-bonded, bilaminate membrane and a polyurethane film were significantly less than control. On the other hand, CFEs for NHKs exposed to a collagen/alginate dressing and to petrolatum-impregnated gauze were significantly greater than control. The choice of a wound dressing carrier has implications for maintaining long-term viability of the transplanted sheet of epithelium.
AB - Cultured keratinocyte grafts transplanted for skin wound repair are often affixed to a wound dressing to facilitate handling. In this study, the ability of five different types of wound dressings to support cell viability and maintain stem cell populations in the cultured grafts was determined. Postconfluent keratinocyte (NHK) sheets were attached to wound dressings for 24 h and then released by trypsinization. Cell viability was determined and NHKs were assessed for clonogenic capacity by colony-forming efficiency (CFE) assays. CFEs for NHKs exposed to a collagen-bonded, bilaminate membrane and a polyurethane film were significantly less than control. On the other hand, CFEs for NHKs exposed to a collagen/alginate dressing and to petrolatum-impregnated gauze were significantly greater than control. The choice of a wound dressing carrier has implications for maintaining long-term viability of the transplanted sheet of epithelium.
KW - Biological dressings
KW - Colony-forming units assay
KW - Keratinocyte transplantation
KW - Stem cell
UR - http://www.scopus.com/inward/record.url?scp=0035696417&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035696417&partnerID=8YFLogxK
M3 - Article
C2 - 11814118
AN - SCOPUS:0035696417
VL - 10
SP - 749
EP - 754
JO - Cell Transplantation
JF - Cell Transplantation
SN - 0963-6897
IS - 8
ER -