Wnt signaling and its downstream target N-myc regulate basal progenitors in the developing neocortex

Atsushi Kuwahara, Yusuke Hirabayashi, Paul S Knoepfler, Makoto M. Taketo, Juro Sakai, Tatsuhiko Kodama, Yukiko Gotoh

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Basal progenitors (also called non-surface dividing or intermediate progenitors) have been proposed to regulate the number of neurons during neocortical development through expanding cells committed to a neuronal fate, although the signals that govern this population have remained largely unknown. Here, we show that N-myc mediates the functions of Wnt signaling in promoting neuronal fate commitment and proliferation of neural precursor cells in vitro. Wnt signaling and N-myc also contribute to the production of basal progenitors in vivo. Expression of a stabilized form of β-catenin, a component of the Wnt signaling pathway, or of N-myc increased the numbers of neocortical basal progenitors, whereas conditional deletion of the N-myc gene reduced these and, as a likely consequence, the number of neocortical neurons. These results reveal that Wnt signaling via N-myc is crucial for the control of neuron number in the developing neocortex.

Original languageEnglish (US)
Pages (from-to)1035-1044
Number of pages10
JournalDevelopment
Volume137
Issue number7
DOIs
StatePublished - Apr 1 2010

Fingerprint

Neocortex
Neurons
Catenins
Wnt Signaling Pathway
myc Genes
Population

Keywords

  • Basal progenitors
  • Mouse
  • N-myc
  • Neocortical development
  • Neural precursor cells
  • The wnt-β-catenin pathway

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Kuwahara, A., Hirabayashi, Y., Knoepfler, P. S., Taketo, M. M., Sakai, J., Kodama, T., & Gotoh, Y. (2010). Wnt signaling and its downstream target N-myc regulate basal progenitors in the developing neocortex. Development, 137(7), 1035-1044. https://doi.org/10.1242/dev.046417

Wnt signaling and its downstream target N-myc regulate basal progenitors in the developing neocortex. / Kuwahara, Atsushi; Hirabayashi, Yusuke; Knoepfler, Paul S; Taketo, Makoto M.; Sakai, Juro; Kodama, Tatsuhiko; Gotoh, Yukiko.

In: Development, Vol. 137, No. 7, 01.04.2010, p. 1035-1044.

Research output: Contribution to journalArticle

Kuwahara, A, Hirabayashi, Y, Knoepfler, PS, Taketo, MM, Sakai, J, Kodama, T & Gotoh, Y 2010, 'Wnt signaling and its downstream target N-myc regulate basal progenitors in the developing neocortex', Development, vol. 137, no. 7, pp. 1035-1044. https://doi.org/10.1242/dev.046417
Kuwahara, Atsushi ; Hirabayashi, Yusuke ; Knoepfler, Paul S ; Taketo, Makoto M. ; Sakai, Juro ; Kodama, Tatsuhiko ; Gotoh, Yukiko. / Wnt signaling and its downstream target N-myc regulate basal progenitors in the developing neocortex. In: Development. 2010 ; Vol. 137, No. 7. pp. 1035-1044.
@article{07d5ede79be843fe93e65d6632b678e1,
title = "Wnt signaling and its downstream target N-myc regulate basal progenitors in the developing neocortex",
abstract = "Basal progenitors (also called non-surface dividing or intermediate progenitors) have been proposed to regulate the number of neurons during neocortical development through expanding cells committed to a neuronal fate, although the signals that govern this population have remained largely unknown. Here, we show that N-myc mediates the functions of Wnt signaling in promoting neuronal fate commitment and proliferation of neural precursor cells in vitro. Wnt signaling and N-myc also contribute to the production of basal progenitors in vivo. Expression of a stabilized form of β-catenin, a component of the Wnt signaling pathway, or of N-myc increased the numbers of neocortical basal progenitors, whereas conditional deletion of the N-myc gene reduced these and, as a likely consequence, the number of neocortical neurons. These results reveal that Wnt signaling via N-myc is crucial for the control of neuron number in the developing neocortex.",
keywords = "Basal progenitors, Mouse, N-myc, Neocortical development, Neural precursor cells, The wnt-β-catenin pathway",
author = "Atsushi Kuwahara and Yusuke Hirabayashi and Knoepfler, {Paul S} and Taketo, {Makoto M.} and Juro Sakai and Tatsuhiko Kodama and Yukiko Gotoh",
year = "2010",
month = "4",
day = "1",
doi = "10.1242/dev.046417",
language = "English (US)",
volume = "137",
pages = "1035--1044",
journal = "Development (Cambridge)",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "7",

}

TY - JOUR

T1 - Wnt signaling and its downstream target N-myc regulate basal progenitors in the developing neocortex

AU - Kuwahara, Atsushi

AU - Hirabayashi, Yusuke

AU - Knoepfler, Paul S

AU - Taketo, Makoto M.

AU - Sakai, Juro

AU - Kodama, Tatsuhiko

AU - Gotoh, Yukiko

PY - 2010/4/1

Y1 - 2010/4/1

N2 - Basal progenitors (also called non-surface dividing or intermediate progenitors) have been proposed to regulate the number of neurons during neocortical development through expanding cells committed to a neuronal fate, although the signals that govern this population have remained largely unknown. Here, we show that N-myc mediates the functions of Wnt signaling in promoting neuronal fate commitment and proliferation of neural precursor cells in vitro. Wnt signaling and N-myc also contribute to the production of basal progenitors in vivo. Expression of a stabilized form of β-catenin, a component of the Wnt signaling pathway, or of N-myc increased the numbers of neocortical basal progenitors, whereas conditional deletion of the N-myc gene reduced these and, as a likely consequence, the number of neocortical neurons. These results reveal that Wnt signaling via N-myc is crucial for the control of neuron number in the developing neocortex.

AB - Basal progenitors (also called non-surface dividing or intermediate progenitors) have been proposed to regulate the number of neurons during neocortical development through expanding cells committed to a neuronal fate, although the signals that govern this population have remained largely unknown. Here, we show that N-myc mediates the functions of Wnt signaling in promoting neuronal fate commitment and proliferation of neural precursor cells in vitro. Wnt signaling and N-myc also contribute to the production of basal progenitors in vivo. Expression of a stabilized form of β-catenin, a component of the Wnt signaling pathway, or of N-myc increased the numbers of neocortical basal progenitors, whereas conditional deletion of the N-myc gene reduced these and, as a likely consequence, the number of neocortical neurons. These results reveal that Wnt signaling via N-myc is crucial for the control of neuron number in the developing neocortex.

KW - Basal progenitors

KW - Mouse

KW - N-myc

KW - Neocortical development

KW - Neural precursor cells

KW - The wnt-β-catenin pathway

UR - http://www.scopus.com/inward/record.url?scp=77950512319&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77950512319&partnerID=8YFLogxK

U2 - 10.1242/dev.046417

DO - 10.1242/dev.046417

M3 - Article

VL - 137

SP - 1035

EP - 1044

JO - Development (Cambridge)

JF - Development (Cambridge)

SN - 0950-1991

IS - 7

ER -