Abstract
Basal progenitors (also called non-surface dividing or intermediate progenitors) have been proposed to regulate the number of neurons during neocortical development through expanding cells committed to a neuronal fate, although the signals that govern this population have remained largely unknown. Here, we show that N-myc mediates the functions of Wnt signaling in promoting neuronal fate commitment and proliferation of neural precursor cells in vitro. Wnt signaling and N-myc also contribute to the production of basal progenitors in vivo. Expression of a stabilized form of β-catenin, a component of the Wnt signaling pathway, or of N-myc increased the numbers of neocortical basal progenitors, whereas conditional deletion of the N-myc gene reduced these and, as a likely consequence, the number of neocortical neurons. These results reveal that Wnt signaling via N-myc is crucial for the control of neuron number in the developing neocortex.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1035-1044 |
| Number of pages | 10 |
| Journal | Development |
| Volume | 137 |
| Issue number | 7 |
| DOIs | |
| State | Published - Apr 1 2010 |
Fingerprint
Keywords
- Basal progenitors
- Mouse
- N-myc
- Neocortical development
- Neural precursor cells
- The wnt-β-catenin pathway
ASJC Scopus subject areas
- Developmental Biology
- Molecular Biology
- Medicine(all)
Cite this
Wnt signaling and its downstream target N-myc regulate basal progenitors in the developing neocortex. / Kuwahara, Atsushi; Hirabayashi, Yusuke; Knoepfler, Paul S; Taketo, Makoto M.; Sakai, Juro; Kodama, Tatsuhiko; Gotoh, Yukiko.
In: Development, Vol. 137, No. 7, 01.04.2010, p. 1035-1044.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Wnt signaling and its downstream target N-myc regulate basal progenitors in the developing neocortex
AU - Kuwahara, Atsushi
AU - Hirabayashi, Yusuke
AU - Knoepfler, Paul S
AU - Taketo, Makoto M.
AU - Sakai, Juro
AU - Kodama, Tatsuhiko
AU - Gotoh, Yukiko
PY - 2010/4/1
Y1 - 2010/4/1
N2 - Basal progenitors (also called non-surface dividing or intermediate progenitors) have been proposed to regulate the number of neurons during neocortical development through expanding cells committed to a neuronal fate, although the signals that govern this population have remained largely unknown. Here, we show that N-myc mediates the functions of Wnt signaling in promoting neuronal fate commitment and proliferation of neural precursor cells in vitro. Wnt signaling and N-myc also contribute to the production of basal progenitors in vivo. Expression of a stabilized form of β-catenin, a component of the Wnt signaling pathway, or of N-myc increased the numbers of neocortical basal progenitors, whereas conditional deletion of the N-myc gene reduced these and, as a likely consequence, the number of neocortical neurons. These results reveal that Wnt signaling via N-myc is crucial for the control of neuron number in the developing neocortex.
AB - Basal progenitors (also called non-surface dividing or intermediate progenitors) have been proposed to regulate the number of neurons during neocortical development through expanding cells committed to a neuronal fate, although the signals that govern this population have remained largely unknown. Here, we show that N-myc mediates the functions of Wnt signaling in promoting neuronal fate commitment and proliferation of neural precursor cells in vitro. Wnt signaling and N-myc also contribute to the production of basal progenitors in vivo. Expression of a stabilized form of β-catenin, a component of the Wnt signaling pathway, or of N-myc increased the numbers of neocortical basal progenitors, whereas conditional deletion of the N-myc gene reduced these and, as a likely consequence, the number of neocortical neurons. These results reveal that Wnt signaling via N-myc is crucial for the control of neuron number in the developing neocortex.
KW - Basal progenitors
KW - Mouse
KW - N-myc
KW - Neocortical development
KW - Neural precursor cells
KW - The wnt-β-catenin pathway
UR - http://www.scopus.com/inward/record.url?scp=77950512319&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77950512319&partnerID=8YFLogxK
U2 - 10.1242/dev.046417
DO - 10.1242/dev.046417
M3 - Article
C2 - 20215343
AN - SCOPUS:77950512319
VL - 137
SP - 1035
EP - 1044
JO - Development (Cambridge)
JF - Development (Cambridge)
SN - 0950-1991
IS - 7
ER -