Abstract
WISP-1 (Wnt-1 induced secreted protein 1) is a member of the CCN family of growth factors. This study identifies WISP-1 as a β-catenin-regulated gene that can contribute to tumorigenesis. The promoter of WISP-1 was cloned and shown to be activated by both Wnt-1 and β-catenin expression. TCF/LEF sites played a minor role, whereas the CREB site played an important role in this transcriptional activation. WISP-1 demonstrated oncogenic activities; overexpression of WISP-1 in normal rat kidney fibroblast cells (NRK-49F) induced morphological transformation, accelerated cell growth, and enhanced saturation density. Although these cells did not acquire anchorage- independent growth in soft agar, they readily formed tumors in nude mice, suggesting that appropriate cellular attachment is important for signaling oncogenic events downstream of WISP-1.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 585-595 |
| Number of pages | 11 |
| Journal | Genes and Development |
| Volume | 14 |
| Issue number | 5 |
| State | Published - Mar 1 2000 |
| Externally published | Yes |
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Keywords
- β-catenin
- Oncogene
- WISP-1
- Wnt-1
ASJC Scopus subject areas
- Genetics
- Developmental Biology
Cite this
WISP-1 is a Wnt-1- and β-catenin-responsive oncogene. / Xu, Lifeng; Corcoran, Ryan B.; Welsh, James W.; Pennica, Diane; Levine, Arnold J.
In: Genes and Development, Vol. 14, No. 5, 01.03.2000, p. 585-595.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - WISP-1 is a Wnt-1- and β-catenin-responsive oncogene
AU - Xu, Lifeng
AU - Corcoran, Ryan B.
AU - Welsh, James W.
AU - Pennica, Diane
AU - Levine, Arnold J.
PY - 2000/3/1
Y1 - 2000/3/1
N2 - WISP-1 (Wnt-1 induced secreted protein 1) is a member of the CCN family of growth factors. This study identifies WISP-1 as a β-catenin-regulated gene that can contribute to tumorigenesis. The promoter of WISP-1 was cloned and shown to be activated by both Wnt-1 and β-catenin expression. TCF/LEF sites played a minor role, whereas the CREB site played an important role in this transcriptional activation. WISP-1 demonstrated oncogenic activities; overexpression of WISP-1 in normal rat kidney fibroblast cells (NRK-49F) induced morphological transformation, accelerated cell growth, and enhanced saturation density. Although these cells did not acquire anchorage- independent growth in soft agar, they readily formed tumors in nude mice, suggesting that appropriate cellular attachment is important for signaling oncogenic events downstream of WISP-1.
AB - WISP-1 (Wnt-1 induced secreted protein 1) is a member of the CCN family of growth factors. This study identifies WISP-1 as a β-catenin-regulated gene that can contribute to tumorigenesis. The promoter of WISP-1 was cloned and shown to be activated by both Wnt-1 and β-catenin expression. TCF/LEF sites played a minor role, whereas the CREB site played an important role in this transcriptional activation. WISP-1 demonstrated oncogenic activities; overexpression of WISP-1 in normal rat kidney fibroblast cells (NRK-49F) induced morphological transformation, accelerated cell growth, and enhanced saturation density. Although these cells did not acquire anchorage- independent growth in soft agar, they readily formed tumors in nude mice, suggesting that appropriate cellular attachment is important for signaling oncogenic events downstream of WISP-1.
KW - β-catenin
KW - Oncogene
KW - WISP-1
KW - Wnt-1
UR - http://www.scopus.com/inward/record.url?scp=0034161886&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034161886&partnerID=8YFLogxK
M3 - Article
C2 - 10716946
AN - SCOPUS:0034161886
VL - 14
SP - 585
EP - 595
JO - Genes and Development
JF - Genes and Development
SN - 0890-9369
IS - 5
ER -