Wilms' tumor with an apparently balanced translocation t(X;18) resulting in deletion of the WTX gene

Moonjoo Han, Miguel N. Rivera, Julie M. Batten, Daniel A. Haber, Paola Dal Cin, A. John Iafrate

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The recent description of a new X chromosome tumor suppressor gene, WTX, that is commonly inactivated in Wilms' tumor prompted us to examine the possible involvement of WTX in a case of Wilms' tumor containing an apparently balanced reciprocal translocation between chromosomes X and 18 (t(X;18)(q11;p11)). Fluorescence in situ hybridization (FISH) analysis of paraffin tumor sections indeed revealed a deletion of the WTX locus at Xq11. High-resolution array comparative genomic hybridization (array CGH) analysis of tumor DNA revealed a 1.5 Mb chromosome deletion encompassing the WTX gene at Xq11. No loss of genetic material was detected on chromosome 18. Interestingly, unlike most tumors with acquired chromosomal translocations, where a new fusion oncogene or promoter-oncogene fusion is created and drives tumor growth, the t(X;18) in this tumor appears to drive tumorigenesis via deletion of a tumor suppressor. This case demonstrates the importance of array CGH and FISH as adjuncts in tumor cytogenetics and in identifying pathogenic microdeletions in "balanced" translocations that are not truly balanced.

Original languageEnglish (US)
Pages (from-to)909-913
Number of pages5
JournalGenes Chromosomes and Cancer
Volume46
Issue number10
DOIs
StatePublished - Oct 1 2007
Externally publishedYes

Fingerprint

Wilms Tumor
Gene Deletion
Oncogene Fusion
Neoplasms
Chromosomes, Human, Pair 18
Comparative Genomic Hybridization
Fluorescence In Situ Hybridization
Chromosome Deletion
Genetic Translocation
X Chromosome
Tumor Suppressor Genes
Cytogenetics
Paraffin
Genes
In Situ Hybridization
Carcinogenesis
DNA
Growth

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

Cite this

Wilms' tumor with an apparently balanced translocation t(X;18) resulting in deletion of the WTX gene. / Han, Moonjoo; Rivera, Miguel N.; Batten, Julie M.; Haber, Daniel A.; Dal Cin, Paola; Iafrate, A. John.

In: Genes Chromosomes and Cancer, Vol. 46, No. 10, 01.10.2007, p. 909-913.

Research output: Contribution to journalArticle

Han, Moonjoo ; Rivera, Miguel N. ; Batten, Julie M. ; Haber, Daniel A. ; Dal Cin, Paola ; Iafrate, A. John. / Wilms' tumor with an apparently balanced translocation t(X;18) resulting in deletion of the WTX gene. In: Genes Chromosomes and Cancer. 2007 ; Vol. 46, No. 10. pp. 909-913.
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