Widefield optical imaging of changes in uptake of glucose and tissue extracellular pH in head and neck cancer

Zhen Luo, Melissa N. Loja, D Gregory Farwell, Quang C. Luu, Paul J. Donald, Deborah Amott, Anh Q. Truong, Regina F Gandour-Edwards, Nitin Nitin

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The overall objective of this study was to develop an optical imaging approach to simultaneously measure altered cell metabolism and changes in tissue extracellular pH with the progression of cancer using clinically isolated biopsies. In this study, 19 pairs of clinically normal and abnormal biopsies were obtained from consenting patients with head and neck cancer at University of California, Davis Medical Center. Fluorescence intensity of tissue biopsies before and after topical delivery of 2-NBDG (2-[N-(7-nitrobenz- 2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose) and Alexa 647-pHLIP [pH (low) insertion peptide] was measured noninvasively by widefield imaging, and correlated with pathologic diagnosis. The results of widefield imaging of clinical biopsies demonstrated that 2-NBDG and pHLIP peptide can accurately distinguish the pathologically normal and abnormal biopsies. The results also demonstrated the potential of this approach to detect subepithelial lesions. Topical application of the contrast agents generated a significant increase in fluorescence contrast (3- To 4-fold) in the cancer biopsies as compared with the normal biopsies, irrespective of the patient and location of the biopsy within a head and neck cavity. This unpaired comparison across all the patients with cancer in this study highlights the specificity of the imaging approach. Furthermore, the results of this study indicated that changes in intracellular glucose metabolism and cancer acidosis are initiated in the early stages of cancer, and these changes are correlated with the progression of the disease. In conclusion, this novel optical molecular imaging approach to measure multiple biomarkers in cancer has a significant potential to be a useful tool for improving early detection and prognostic evaluation of oral neoplasia.

Original languageEnglish (US)
Pages (from-to)1035-1044
Number of pages10
JournalCancer Prevention Research
Volume7
Issue number10
DOIs
StatePublished - Oct 1 2014

Fingerprint

Optical Imaging
Head and Neck Neoplasms
Biopsy
Glucose
Neoplasms
Fluorescence
Molecular Imaging
Tumor Biomarkers
Acidosis
Contrast Media
Disease Progression
Neck
Head

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Widefield optical imaging of changes in uptake of glucose and tissue extracellular pH in head and neck cancer. / Luo, Zhen; Loja, Melissa N.; Farwell, D Gregory; Luu, Quang C.; Donald, Paul J.; Amott, Deborah; Truong, Anh Q.; Gandour-Edwards, Regina F; Nitin, Nitin.

In: Cancer Prevention Research, Vol. 7, No. 10, 01.10.2014, p. 1035-1044.

Research output: Contribution to journalArticle

Luo, Zhen ; Loja, Melissa N. ; Farwell, D Gregory ; Luu, Quang C. ; Donald, Paul J. ; Amott, Deborah ; Truong, Anh Q. ; Gandour-Edwards, Regina F ; Nitin, Nitin. / Widefield optical imaging of changes in uptake of glucose and tissue extracellular pH in head and neck cancer. In: Cancer Prevention Research. 2014 ; Vol. 7, No. 10. pp. 1035-1044.
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