Wide-field two-photon microscopy: Features and advantages for biomedical applications

Sebastian Wachsmann-Hogiu, J. Y. Hwang, E. Lindsley, D. L. Farkas

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

We describe a simple fluorescence microscope based on wide-field two-photon excitation. While still taking advantage of some inherent properties of non-linear (two-photon) microscopy, such as increased penetration depth through tissue and reduced phototoxicity, this approach provides video frame rate imaging, can be easily coupled to fluorescence spectral and lifetime detection modules, and makes efficient use of the high average power currently available from ultrashort pulsed lasers. For a standard histopathology specimen, we were able to identify different structures based on spectral and fluorescence lifetime detection and analysis. We examined the use of 200fs and 2ps pulses from Spectra Physics MaiTai and Tsunami lasers, respectively, with average power ranging from 50mW to 500mW.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6441
DOIs
StatePublished - 2007
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V - San Jose, CA, United States
Duration: Jan 22 2007Jan 24 2007

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
CountryUnited States
CitySan Jose, CA
Period1/22/071/24/07

Keywords

  • Confocal microscopy
  • Fluorescence lifetime imaging microscopy (FLIM)
  • Spectral imaging
  • Two-photon excitation
  • Wide-field fluorescence
  • Wide-field two-photon excitation

ASJC Scopus subject areas

  • Engineering(all)

Fingerprint Dive into the research topics of 'Wide-field two-photon microscopy: Features and advantages for biomedical applications'. Together they form a unique fingerprint.

  • Cite this

    Wachsmann-Hogiu, S., Hwang, J. Y., Lindsley, E., & Farkas, D. L. (2007). Wide-field two-photon microscopy: Features and advantages for biomedical applications. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6441). [64411B] https://doi.org/10.1117/12.711603