Who's behind that mask and cape? the Asian leopard cat's Agouti (ASIP) allele likely affects coat colour phenotype in the Bengal cat breed

L. C. Gershony, Cecilia Penedo, B. W. Davis, W. J. Murphy, C. R. Helps, Leslie A Lyons

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Coat colours and patterns are highly variable in cats and are determined mainly by several genes with Mendelian inheritance. A 2-bp deletion in agouti signalling protein (ASIP) is associated with melanism in domestic cats. Bengal cats are hybrids between domestic cats and Asian leopard cats (Prionailurus bengalensis), and the charcoal coat colouration/pattern in Bengals presents as a possible incomplete melanism. The complete coding region of ASIP was directly sequenced in Asian leopard, domestic and Bengal cats. Twenty-seven variants were identified between domestic and leopard cats and were investigated in Bengals and Savannahs, a hybrid with servals (Leptailurus serval). The leopard cat ASIP haplotype was distinguished from domestic cat by four synonymous and four non-synonymous exonic SNPs, as well as 19 intronic variants, including a 42-bp deletion in intron 4. Fifty-six of 64 reported charcoal cats were compound heterozygotes at ASIP, with leopard cat agouti (AP be) and domestic cat non-agouti (a) haplotypes. Twenty-four Bengals had an additional unique haplotype (A2) for exon 2 that was not identified in leopard cats, servals or jungle cats (Felis chaus). The compound heterozygote state suggests the leopard cat allele, in combination with the recessive non-agouti allele, influences Bengal markings, producing a darker, yet not completely melanistic coat. This is the first validation of a leopard cat allele segregating in the Bengal breed and likely affecting their overall pelage phenotype. Genetic testing services need to be aware of the possible segregation of wild felid alleles in all assays performed on hybrid cats.

Original languageEnglish (US)
Pages (from-to)893-897
Number of pages5
JournalAnimal Genetics
Volume45
Issue number6
DOIs
StatePublished - Dec 1 2014

Fingerprint

Agouti Signaling Protein
Prionailurus bengalensis
Panthera
Agouti
Masks
Cats
Color
Alleles
cats
alleles
Phenotype
phenotype
color
proteins
haplotypes
charcoal
heterozygosity
Haplotypes
Melanosis
Dasyproctidae

Keywords

  • charcoal
  • domestic cat
  • felid
  • Felis silvestris catus
  • hybrid
  • jungle cat
  • pelage
  • serval

ASJC Scopus subject areas

  • Genetics
  • Animal Science and Zoology
  • Medicine(all)

Cite this

Who's behind that mask and cape? the Asian leopard cat's Agouti (ASIP) allele likely affects coat colour phenotype in the Bengal cat breed. / Gershony, L. C.; Penedo, Cecilia; Davis, B. W.; Murphy, W. J.; Helps, C. R.; Lyons, Leslie A.

In: Animal Genetics, Vol. 45, No. 6, 01.12.2014, p. 893-897.

Research output: Contribution to journalArticle

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abstract = "Coat colours and patterns are highly variable in cats and are determined mainly by several genes with Mendelian inheritance. A 2-bp deletion in agouti signalling protein (ASIP) is associated with melanism in domestic cats. Bengal cats are hybrids between domestic cats and Asian leopard cats (Prionailurus bengalensis), and the charcoal coat colouration/pattern in Bengals presents as a possible incomplete melanism. The complete coding region of ASIP was directly sequenced in Asian leopard, domestic and Bengal cats. Twenty-seven variants were identified between domestic and leopard cats and were investigated in Bengals and Savannahs, a hybrid with servals (Leptailurus serval). The leopard cat ASIP haplotype was distinguished from domestic cat by four synonymous and four non-synonymous exonic SNPs, as well as 19 intronic variants, including a 42-bp deletion in intron 4. Fifty-six of 64 reported charcoal cats were compound heterozygotes at ASIP, with leopard cat agouti (AP be) and domestic cat non-agouti (a) haplotypes. Twenty-four Bengals had an additional unique haplotype (A2) for exon 2 that was not identified in leopard cats, servals or jungle cats (Felis chaus). The compound heterozygote state suggests the leopard cat allele, in combination with the recessive non-agouti allele, influences Bengal markings, producing a darker, yet not completely melanistic coat. This is the first validation of a leopard cat allele segregating in the Bengal breed and likely affecting their overall pelage phenotype. Genetic testing services need to be aware of the possible segregation of wild felid alleles in all assays performed on hybrid cats.",
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