Whole-genome transcriptional analysis of chemolithoautotrophic thiosulfate oxidation by Thiobacillus denitrificans under aerobic versus denitrifying conditions

Harry R. Beller, Tracy E. Letain, Anu Chakicherla, Staci R. Kane, Tina C. Legler, Matthew A Coleman

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Thiobacillus denitrificans is one of the few known obligate chemolithoautotrophic bacteria capable of energetically coupling thiosulfate oxidation to denitrification as well as aerobic respiration. As very little is known about the differential expression of genes associated with key chemolithoautotrophic functions (such as sulfur compound oxidation and CO 2 fixation) under aerobic versus denitrifying conditions, we conducted whole-genome, cDNA microarray studies to explore this topic systematically. The microarrays identified 277 genes (approximately 10% of the genome) as differentially expressed using RMA (robust multiarray average) statistical analysis and a twofold cutoff. Genes upregulated (ca. 6- to 150-fold) under aerobic conditions included a cluster of genes associated with iron acquisition (e.g., siderophore-related genes), a cluster of cytochrome cbb3 oxidase genes, cbbL and cbbS (encoding the large and small subunits of form I ribulose 1,5-bisphosphate carboxylase/oxygenase, or RubisCO), and multiple molecular chaperone genes. Genes upregulated (ca. 4- to 95-fold) under denitrifying conditions included nar, nir, and nor genes (associated, respectively, with nitrate reductase, nitrite reductase, and nitric oxide reductase, which catalyze successive steps of denitrification), cbbM (encoding form II RubisCO), and genes involved with sulfur compound oxidation (including two physically separated but highly similar copies of sulfide:quinone oxidoreductase and of dsrC, associated with dissimilatory sulfite reductase). Among genes associated with denitrification, relative expression levels (i.e., degree of upregulation with nitrate) tended to decrease in the order nar > nir > nor > nos. Reverse transcription-quantitative PCR analysis was used to validate these trends.

Original languageEnglish (US)
Pages (from-to)7005-7015
Number of pages11
JournalJournal of Bacteriology
Volume188
Issue number19
DOIs
StatePublished - Oct 2006
Externally publishedYes

Fingerprint

Thiobacillus
Thiosulfates
Genome
Denitrification
Genes
Ribulose-Bisphosphate Carboxylase
Sulfur Compounds
sulfide quinone reductase
Multigene Family
Hydrogensulfite Reductase
Nitrite Reductases
Nitrate Reductase
Siderophores
Oxygenases
Molecular Chaperones
Carbon Monoxide
Oligonucleotide Array Sequence Analysis
Nitrates
Reverse Transcription
Respiration

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Whole-genome transcriptional analysis of chemolithoautotrophic thiosulfate oxidation by Thiobacillus denitrificans under aerobic versus denitrifying conditions. / Beller, Harry R.; Letain, Tracy E.; Chakicherla, Anu; Kane, Staci R.; Legler, Tina C.; Coleman, Matthew A.

In: Journal of Bacteriology, Vol. 188, No. 19, 10.2006, p. 7005-7015.

Research output: Contribution to journalArticle

Beller, Harry R. ; Letain, Tracy E. ; Chakicherla, Anu ; Kane, Staci R. ; Legler, Tina C. ; Coleman, Matthew A. / Whole-genome transcriptional analysis of chemolithoautotrophic thiosulfate oxidation by Thiobacillus denitrificans under aerobic versus denitrifying conditions. In: Journal of Bacteriology. 2006 ; Vol. 188, No. 19. pp. 7005-7015.
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