Whole genome deoxyribonucleic acid microarray analysis of gene expression in ectopic versus eutopic endometrium

Kathleen M. Eyster, Olga Klinkova, Vanessa A Kennedy, Keith A. Hansen

Research output: Contribution to journalArticle

131 Scopus citations

Abstract

Objective: To use DNA microarrays to identify differentially expressed genes in eutopic endometrium compared with ectopic endometrium. Design: Prospective, cross-sectional, observational study. Setting: University Medical Center and Research Laboratory. Patient(s): Eleven women with endometriosis. Intervention(s): None. Main Outcome Measure(s): Differential gene expression. Result(s): Seven hundred seventeen of the 53,000 probes on the whole human DNA microarrays were changed by twofold or greater in ectopic versus eutopic endometrium. Families of genes that were expressed differentially include genes that code for proteins associated with the immune system and inflammatory pathways, cell adhesion, cell-cell junctions, the extracellular matrix and its remodeling, cytoskeletal proteins, and signal transduction pathway components, among others. Conclusion(s): The altered immune environment may allow survival of endometriotic cells that enter the abdominal cavity. Alterations of cell adhesion-associated genes may contribute to the adhesive and invasive properties of ectopic endometrium, and changes in signal transduction pathways support a change in the communication among cells of the endometrial explant compared with eutopic endometrium. These families of differentially expressed genes provide multiple opportunities for the development and testing of new hypotheses regarding endometriosis.

Original languageEnglish (US)
Pages (from-to)1505-1533
Number of pages29
JournalFertility and Sterility
Volume88
Issue number6
DOIs
StatePublished - Dec 2007
Externally publishedYes

Keywords

  • differential gene expression
  • DNA microarray
  • Endometriosis
  • phospholipase A

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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