White sturgeon as a potential vector of infectious hematopoietic necrosis virus

S. E. Lapatra, G. R. Jones, K. A. Lauda, T. S. McDowell, R. Schneider, Ronald Hedrick

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cell lines from white sturgeon Acipenser transmontanus were derived from peripheral blood cells, heart, and spleen. Incubated with infectious hematopoietic necrosis virus (IHNV) for 8 d at l5 ° C, these cell lines produced 0.7–53.2 plaque-forming units (PFU)/cell. Waterborne exposure of larval white sturgeons (60 d posthatch) to 106 PFU/mL of IHNV resulted in 10% mortality 5–6 d postinfection, with virus concentrations consistently greater than 10 5 PFU/g. A replicate group of larval white sturgeons that were sampled at different times post-IHNV exposure had no detectable virus at 24 h, but 72% of the fish had IHNV concentrations of 102–106 PFU/g when they were examined 2–9 d postinfection. Juvenile white sturgeons (mean weight, 35 g) immersed in or injected with IHNV exhibited no mortality, and virus was only detected immediately postexposure in just 25% of the fish tested. Juvenile white sturgeons fed either virus-free rainbow trout Oncorhynchus mykiss or dead IHNV-infected rainbow trout had no viable virus in their feces. Juvenile white sturgeons fed or exposed to IHNV failed to transmit the virus to cohabiting rainbow trout fry. These results suggest that IHNV can replicate in larval white sturgeons but presumably not in juveniles or adults. Virus neutralization activity was detected in serum from adult white sturgeons (4–6 years old) cultured with rainbow trout exposed to IHNV but not in white sturgeons kept in a pathogen-free environment and fed a manufactured diet. White sturgeon serum with IHNV-neutralizing activity was used to passively immunize rainbow trout, and it provided significant (P < 0.01) protection against IHNV challenge.

Original languageEnglish (US)
Pages (from-to)225-230
Number of pages6
JournalJournal of Aquatic Animal Health
Volume7
Issue number3
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

Fingerprint

Acipenser transmontanus
Infectious hematopoietic necrosis virus
virus
Oncorhynchus mykiss
viruses
rainbow
necrosis
cell lines
neutralization tests
serum
blood cells
fish
fish fry
neutralization
spleen
mortality
feces
heart

ASJC Scopus subject areas

  • Aquatic Science

Cite this

White sturgeon as a potential vector of infectious hematopoietic necrosis virus. / Lapatra, S. E.; Jones, G. R.; Lauda, K. A.; McDowell, T. S.; Schneider, R.; Hedrick, Ronald.

In: Journal of Aquatic Animal Health, Vol. 7, No. 3, 01.01.1995, p. 225-230.

Research output: Contribution to journalArticle

Lapatra, S. E. ; Jones, G. R. ; Lauda, K. A. ; McDowell, T. S. ; Schneider, R. ; Hedrick, Ronald. / White sturgeon as a potential vector of infectious hematopoietic necrosis virus. In: Journal of Aquatic Animal Health. 1995 ; Vol. 7, No. 3. pp. 225-230.
@article{d9adb16b590e4a6e9a801820b598ebbc,
title = "White sturgeon as a potential vector of infectious hematopoietic necrosis virus",
abstract = "Cell lines from white sturgeon Acipenser transmontanus were derived from peripheral blood cells, heart, and spleen. Incubated with infectious hematopoietic necrosis virus (IHNV) for 8 d at l5 ° C, these cell lines produced 0.7–53.2 plaque-forming units (PFU)/cell. Waterborne exposure of larval white sturgeons (60 d posthatch) to 106 PFU/mL of IHNV resulted in 10{\%} mortality 5–6 d postinfection, with virus concentrations consistently greater than 10 5 PFU/g. A replicate group of larval white sturgeons that were sampled at different times post-IHNV exposure had no detectable virus at 24 h, but 72{\%} of the fish had IHNV concentrations of 102–106 PFU/g when they were examined 2–9 d postinfection. Juvenile white sturgeons (mean weight, 35 g) immersed in or injected with IHNV exhibited no mortality, and virus was only detected immediately postexposure in just 25{\%} of the fish tested. Juvenile white sturgeons fed either virus-free rainbow trout Oncorhynchus mykiss or dead IHNV-infected rainbow trout had no viable virus in their feces. Juvenile white sturgeons fed or exposed to IHNV failed to transmit the virus to cohabiting rainbow trout fry. These results suggest that IHNV can replicate in larval white sturgeons but presumably not in juveniles or adults. Virus neutralization activity was detected in serum from adult white sturgeons (4–6 years old) cultured with rainbow trout exposed to IHNV but not in white sturgeons kept in a pathogen-free environment and fed a manufactured diet. White sturgeon serum with IHNV-neutralizing activity was used to passively immunize rainbow trout, and it provided significant (P < 0.01) protection against IHNV challenge.",
author = "Lapatra, {S. E.} and Jones, {G. R.} and Lauda, {K. A.} and McDowell, {T. S.} and R. Schneider and Ronald Hedrick",
year = "1995",
month = "1",
day = "1",
doi = "10.1577/1548-8667(1995)007<0225:WSAAPV>2.3.CO;2",
language = "English (US)",
volume = "7",
pages = "225--230",
journal = "Journal of Aquatic Animal Health",
issn = "0899-7659",
publisher = "American Fisheries Society",
number = "3",

}

TY - JOUR

T1 - White sturgeon as a potential vector of infectious hematopoietic necrosis virus

AU - Lapatra, S. E.

AU - Jones, G. R.

AU - Lauda, K. A.

AU - McDowell, T. S.

AU - Schneider, R.

AU - Hedrick, Ronald

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Cell lines from white sturgeon Acipenser transmontanus were derived from peripheral blood cells, heart, and spleen. Incubated with infectious hematopoietic necrosis virus (IHNV) for 8 d at l5 ° C, these cell lines produced 0.7–53.2 plaque-forming units (PFU)/cell. Waterborne exposure of larval white sturgeons (60 d posthatch) to 106 PFU/mL of IHNV resulted in 10% mortality 5–6 d postinfection, with virus concentrations consistently greater than 10 5 PFU/g. A replicate group of larval white sturgeons that were sampled at different times post-IHNV exposure had no detectable virus at 24 h, but 72% of the fish had IHNV concentrations of 102–106 PFU/g when they were examined 2–9 d postinfection. Juvenile white sturgeons (mean weight, 35 g) immersed in or injected with IHNV exhibited no mortality, and virus was only detected immediately postexposure in just 25% of the fish tested. Juvenile white sturgeons fed either virus-free rainbow trout Oncorhynchus mykiss or dead IHNV-infected rainbow trout had no viable virus in their feces. Juvenile white sturgeons fed or exposed to IHNV failed to transmit the virus to cohabiting rainbow trout fry. These results suggest that IHNV can replicate in larval white sturgeons but presumably not in juveniles or adults. Virus neutralization activity was detected in serum from adult white sturgeons (4–6 years old) cultured with rainbow trout exposed to IHNV but not in white sturgeons kept in a pathogen-free environment and fed a manufactured diet. White sturgeon serum with IHNV-neutralizing activity was used to passively immunize rainbow trout, and it provided significant (P < 0.01) protection against IHNV challenge.

AB - Cell lines from white sturgeon Acipenser transmontanus were derived from peripheral blood cells, heart, and spleen. Incubated with infectious hematopoietic necrosis virus (IHNV) for 8 d at l5 ° C, these cell lines produced 0.7–53.2 plaque-forming units (PFU)/cell. Waterborne exposure of larval white sturgeons (60 d posthatch) to 106 PFU/mL of IHNV resulted in 10% mortality 5–6 d postinfection, with virus concentrations consistently greater than 10 5 PFU/g. A replicate group of larval white sturgeons that were sampled at different times post-IHNV exposure had no detectable virus at 24 h, but 72% of the fish had IHNV concentrations of 102–106 PFU/g when they were examined 2–9 d postinfection. Juvenile white sturgeons (mean weight, 35 g) immersed in or injected with IHNV exhibited no mortality, and virus was only detected immediately postexposure in just 25% of the fish tested. Juvenile white sturgeons fed either virus-free rainbow trout Oncorhynchus mykiss or dead IHNV-infected rainbow trout had no viable virus in their feces. Juvenile white sturgeons fed or exposed to IHNV failed to transmit the virus to cohabiting rainbow trout fry. These results suggest that IHNV can replicate in larval white sturgeons but presumably not in juveniles or adults. Virus neutralization activity was detected in serum from adult white sturgeons (4–6 years old) cultured with rainbow trout exposed to IHNV but not in white sturgeons kept in a pathogen-free environment and fed a manufactured diet. White sturgeon serum with IHNV-neutralizing activity was used to passively immunize rainbow trout, and it provided significant (P < 0.01) protection against IHNV challenge.

UR - http://www.scopus.com/inward/record.url?scp=0028836148&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028836148&partnerID=8YFLogxK

U2 - 10.1577/1548-8667(1995)007<0225:WSAAPV>2.3.CO;2

DO - 10.1577/1548-8667(1995)007<0225:WSAAPV>2.3.CO;2

M3 - Article

VL - 7

SP - 225

EP - 230

JO - Journal of Aquatic Animal Health

JF - Journal of Aquatic Animal Health

SN - 0899-7659

IS - 3

ER -