What causes canine sino-nasal aspergillosis? A molecular approach to species identification

Jessica J. Talbot, Lynelle R Johnson, Patricia Martin, Julia A. Beatty, Deanna A. Sutton, Frédéric Billen, Catriona L. Halliday, Justine S. Gibson, Sarah Kidd, Jörg M. Steiner, Beata Ujvari, Vanessa R. Barrs

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

On the basis of phenotypic identification methods, Aspergillus fumigatus is reported as the most commonly identified aetiological agent of canine sino-nasal aspergillosis (SNA). However, definitive identification of Aspergillus spp. using phenotypic features alone is unreliable. The aim of this study was to determine the molecular identities of fungal species causing SNA in dogs. Genomic DNA was extracted from 91 fungal isolates from 90 dogs diagnosed with SNA in Australia, the USA and Belgium, and the ITS1-5.8S-ITS2 ribosomal DNA and partial β-tubulin regions were sequenced. Eighty-eight of 91 (96.7%) isolates were identified as A. fumigatus and 3/91 (3.3%) belonged to Aspergillus section Nigri spp. (Aspergillus tubingensis: 2/91; Aspergillus uvarum: 1/91). These findings confirm that A. fumigatus is the most common aetiological agent of canine SNA. This is the first report to document a pathogenic role for A. tubingensis and A. uvarum in dogs.

Original languageEnglish (US)
Pages (from-to)17-21
Number of pages5
JournalVeterinary Journal
Volume200
Issue number1
DOIs
StatePublished - 2014

Fingerprint

Aspergillosis
aspergillosis
Aspergillus
Nose
Aspergillus fumigatus
Canidae
Aspergillus tubingensis
dogs
Dogs
etiological agents
Belgium
Tubulin
Ribosomal DNA
tubulin
ribosomal DNA
species identification
DNA
genomics

Keywords

  • Aspergillus fumigatus
  • Aspergillus tubingensis
  • Aspergillus uvarum
  • Canine
  • Sino-nasal aspergillosis

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Talbot, J. J., Johnson, L. R., Martin, P., Beatty, J. A., Sutton, D. A., Billen, F., ... Barrs, V. R. (2014). What causes canine sino-nasal aspergillosis? A molecular approach to species identification. Veterinary Journal, 200(1), 17-21. https://doi.org/10.1016/j.tvjl.2014.01.009

What causes canine sino-nasal aspergillosis? A molecular approach to species identification. / Talbot, Jessica J.; Johnson, Lynelle R; Martin, Patricia; Beatty, Julia A.; Sutton, Deanna A.; Billen, Frédéric; Halliday, Catriona L.; Gibson, Justine S.; Kidd, Sarah; Steiner, Jörg M.; Ujvari, Beata; Barrs, Vanessa R.

In: Veterinary Journal, Vol. 200, No. 1, 2014, p. 17-21.

Research output: Contribution to journalArticle

Talbot, JJ, Johnson, LR, Martin, P, Beatty, JA, Sutton, DA, Billen, F, Halliday, CL, Gibson, JS, Kidd, S, Steiner, JM, Ujvari, B & Barrs, VR 2014, 'What causes canine sino-nasal aspergillosis? A molecular approach to species identification', Veterinary Journal, vol. 200, no. 1, pp. 17-21. https://doi.org/10.1016/j.tvjl.2014.01.009
Talbot, Jessica J. ; Johnson, Lynelle R ; Martin, Patricia ; Beatty, Julia A. ; Sutton, Deanna A. ; Billen, Frédéric ; Halliday, Catriona L. ; Gibson, Justine S. ; Kidd, Sarah ; Steiner, Jörg M. ; Ujvari, Beata ; Barrs, Vanessa R. / What causes canine sino-nasal aspergillosis? A molecular approach to species identification. In: Veterinary Journal. 2014 ; Vol. 200, No. 1. pp. 17-21.
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