Western equine encephalomyelitis virus infection affects the life table characteristics of Culex tarsalis (Diptera

Culicidae)

Farida Mahmood, William Reisen, Robert E. Chiles, Ying Fang

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The life table attributes of Culex tarsalis Coquillett females infected experimentally by feeding on 4 and 6 log10 plaque-forming units (PFU) of western equine encephalomyelitis virus (WEEV) per milliliter of heparinized chicken blood were compared with an uninfected control group. Females continually were offered 10% sucrose and an oviposition substrate and daily a blood meal through a biomembrane feeder. Mortality (dead females) and fecundity (female eggs per female) were monitored daily until all females died. Overall, 94% of 198 females in the two virus-infected groups were positive for WEEV at death when tested by plaque assay; the average body virus titer at death did not differ between groups. WEEV infection significantly altered the life table characteristics of Cx. tarsalis. Life expectancy at infection in days (e x), reproductive effort in female eggs per female per generation (Ro), and generation time (T) in days for the infected cohorts were significantly lower than for the uninfected controls, whereas the reproductive rate (rc) in female eggs per female per day was higher for infected than uninfected cohorts. In agreement with the WEEV infection data that showed similar body titers, there were few differences between the life table parameters for the 4 and 6 log10 PFU treatment groups. Greatest differences were observed for survivorship between days 17-40 when virus titers in infected dying females were greatest. Our data extend recent studies that indicate mosquito infection with encephalitis viruses has a cost of reduced life expectancy and fitness.

Original languageEnglish (US)
Pages (from-to)982-986
Number of pages5
JournalJournal of Medical Entomology
Volume41
Issue number5
DOIs
StatePublished - Jan 1 2004

Fingerprint

Equine Encephalomyelitis
Western Equine Encephalitis Viruses
Western equine encephalitis virus
Culex tarsalis
Culex
Life Tables
life tables
Culicidae
Diptera
infection
Eggs
viral load
Life Expectancy
Viral Load
Encephalitis Viruses
death
viruses
Oviposition
blood meal
encephalitis

Keywords

  • Culex tarsalis
  • Fitness
  • Life table
  • Survivorship
  • Western equine encephalomyelitis virus

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

Cite this

Western equine encephalomyelitis virus infection affects the life table characteristics of Culex tarsalis (Diptera : Culicidae). / Mahmood, Farida; Reisen, William; Chiles, Robert E.; Fang, Ying.

In: Journal of Medical Entomology, Vol. 41, No. 5, 01.01.2004, p. 982-986.

Research output: Contribution to journalArticle

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