West Nile virus detection in nonvascular feathers from avian carcasses

Nicole M. Nemeth, Ginger R. Young, Kristen L. Burkhalter, Aaron Brault, William Reisen, Nicholas Komar

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

West Nile virus (WNV) is a public health threat and has caused the death of thousands of North American birds. As such, surveillance for WNV has been ongoing, utilizing numerous biological specimens and testing methods. Nonvascular (i.e., fully grown) feathers would provide a simple method of collection from either dead or live birds of all ages and molt cycles, with presumably less biosafety risk compared with other specimen types, including feather pulp. The current study evaluates WNV detection in nonvascular feathers removed from naturally infected avian carcasses of several species groups. Feathers of corvid passeriforms had the highest sensitivity of detection (64%), followed by noncorvid passeriforms (43%), columbiforms (33%), and falconiforms (31%). Storing feathers for 1 year at -20°C or at ambient room temperature resulted in detection rates of infectious WNV of 16% and zero, respectively, but had no effect on detection rates of WNV RNA in a subset of matched feather pairs (47% for both storage temperatures). The efficacy of WNV detection in nonvascular feathers is greatly enhanced by testing multiple feathers. The advantages of using nonvascular feathers over other tissues may outweigh the relatively low detectability of WNV RNA in certain situations such as remote areas lacking resources for acquiring other types of samples or maintaining the cold chain.

Original languageEnglish (US)
Pages (from-to)616-622
Number of pages7
JournalJournal of Veterinary Diagnostic Investigation
Volume21
Issue number5
DOIs
StatePublished - Jan 1 2009

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West Nile virus
Feathers
feathers
Birds
RNA
Refrigeration
biosafety
Temperature
birds
type collections
storage temperature
pulp
molting
ambient temperature
public health
Public Health
testing
death
monitoring

Keywords

  • Birds
  • Diagnosis
  • Feather
  • Reverse transcription polymerase chain reaction
  • West Nile virus

ASJC Scopus subject areas

  • veterinary(all)

Cite this

West Nile virus detection in nonvascular feathers from avian carcasses. / Nemeth, Nicole M.; Young, Ginger R.; Burkhalter, Kristen L.; Brault, Aaron; Reisen, William; Komar, Nicholas.

In: Journal of Veterinary Diagnostic Investigation, Vol. 21, No. 5, 01.01.2009, p. 616-622.

Research output: Contribution to journalArticle

Nemeth, Nicole M. ; Young, Ginger R. ; Burkhalter, Kristen L. ; Brault, Aaron ; Reisen, William ; Komar, Nicholas. / West Nile virus detection in nonvascular feathers from avian carcasses. In: Journal of Veterinary Diagnostic Investigation. 2009 ; Vol. 21, No. 5. pp. 616-622.
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