Wavefront sensorless adaptive optics fluorescence biomicroscope for in vivo retinal imaging in mice

Daniel J. Wahl, Yifan Jian, Stefano Bonora, Robert Zawadzki, Marinko V. Sarunic

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Cellular-resolution in vivo fluorescence imaging is a valuable tool for longitudinal studies of retinal function in vision research. Wavefront sensorless adaptive optics (WSAO) is a developing technology that enables high-resolution imaging of the mouse retina. In place of the conventional method of using a Shack-Hartmann wavefront sensor to measure the aberrations directly, WSAO uses an image quality metric and a search algorithm to drive the shape of the adaptive element (i.e. deformable mirror). WSAO is a robust approach to AO and it is compatible with a compact, low-cost lens-based system. In this report, we demonstrated a hill-climbing algorithm for WSAO with a variable focus lens and deformable mirror for non-invasive in vivo imaging of EGFP (enhanced green fluorescent protein) labelled ganglion cells and microglia cells in the mouse retina.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalBiomedical Optics Express
Volume7
Issue number1
DOIs
StatePublished - Dec 4 2015

Fingerprint

adaptive optics
Lenses
mice
Retina
Fluorescence
fluorescence
retina
deformable mirrors
Optical Imaging
Microglia
Ganglia
Longitudinal Studies
lenses
Technology
Costs and Cost Analysis
cells
Research
aberration
mirrors
proteins

Keywords

  • Active or adaptive optics
  • Imaging systems
  • Ophthalmic optics and devices
  • Ophthalmology

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Wavefront sensorless adaptive optics fluorescence biomicroscope for in vivo retinal imaging in mice. / Wahl, Daniel J.; Jian, Yifan; Bonora, Stefano; Zawadzki, Robert; Sarunic, Marinko V.

In: Biomedical Optics Express, Vol. 7, No. 1, 04.12.2015, p. 1-12.

Research output: Contribution to journalArticle

Wahl, Daniel J. ; Jian, Yifan ; Bonora, Stefano ; Zawadzki, Robert ; Sarunic, Marinko V. / Wavefront sensorless adaptive optics fluorescence biomicroscope for in vivo retinal imaging in mice. In: Biomedical Optics Express. 2015 ; Vol. 7, No. 1. pp. 1-12.
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