Warburg effect linked to cognitive-executive deficits in FMR1 premutation

Eleonora Napoli, Gyu Song, Andrea Schneider, Randi J Hagerman, Marwa Abd Al Azaim Eldeeb, Atoosa Azarang, Flora Tassone, Cecilia R Giulivi

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

A55-200CGGrepeat expansion in the 59-UTR of the fragileXmental retardation 1 (FMR1) gene isknown as a premutation. Some carriers are affected by the neurodegenerative disorder fragile X-associated tremor/ataxia syndrome (FXTAS), primary ovarian insufficiency, and neurobehavioral impairments. Based on themitochondrial dysfunction observed in fibroblasts and brain samples from carriers, as well as in neurons and brains from a mouse model of the premutation, we evaluated the presence of theWarburg effect in peripheral bloodmononuclear cells (PBMCs) from 30 premutation carriers with either a rebalance of the metabolism [increasing glycolysis while decreasing oxidative phosphorylation (oxphos)] or a metabolic amplification (increasing glycolysis while maintaining/increasing oxphos). Deficits in oxphos-more pronounced in FXTAS-affected subjects-were accompanied by a shift toward glycolysis, suggesting increased glycolysis despite aerobic conditions. Differential proteomics extended these findings, unveiling a decreased antioxidant response, translation, and disrupted extracellular matrix and cytoskeleton organization with activation of prosenescence pathways. Lower bioenergetics segregated with increased incidence of low executive function, tremors, below-average IQ, and FXTAS. The combination of functional and proteomic data unveiled new mechanisms related to energy production in the premutation, showing the potential of being applicable to other psychiatric disorders to identify endophenotypespecific responses relevant to neurobiology.-Napoli, E., Song,G.,Schneider,A.,Hagerman,R., Eldeeb,M.A.A.A., Azarang, A., Tassone, F., Giulivi, C. Warburg effect linked to cognitive-executive deficits in FMR1 premutation.

Original languageEnglish (US)
Pages (from-to)3334-3351
Number of pages18
JournalFASEB Journal
Volume30
Issue number10
DOIs
StatePublished - Oct 1 2016

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Keywords

  • Fragile X
  • Mitochondria
  • Neurological disorder
  • Proteomics
  • Triplet nucleotide diseases

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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