Volumetric retinal imaging with ultrahigh-resolution spectral-domain optical coherence tomography and adaptive optics using two broadband light sources

Barry Cense, Eric Koperda, Jeffrey M. Brown, Omer P. Kocaoglu, Weihua Gao, Ravi Jonnal, Donald T. Miller

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Ultrabroadband sources, such as multiplexed superluminescent diodes (SLDs) and femtosecond lasers, have been successfully employed in adaptive optics optical coherence tomography (AO-OCT) systems for ultrahigh resolution retinal imaging. The large cost differential of these sources, however, motivates the need for a performance comparison. Here, we compare the performance of a Femtolasers Integral Ti: Sapphire laser and a Superlum BroadLighter T840, using the same AO-OCT system and the same subject. In addition, we investigate the capability of our instrument equipped with the Integral to capture volume images of the fovea and adjacent regions on a second subject using the AO to control focus in the retina and custom and freeware image registration software to reduce eye motion artifacts. Monochromatic ocular aberrations were corrected with a woofer-tweeter AO system. Coherence lengths of the Integral and BroadLighter were measured in vivo at 3.2 μm and 3.3 μm, respectively. The difference in dynamic range was 5 dB, close to the expected variability of the experiment. Individual cone photoreceptors, retinal capillaries and nerve fiber bundles were distinguished in all three dimensions with both sources. The acquired retinal volumes are provided for viewing in OSA ISP, allowing the reader to data mine at the microscope level.

Original languageEnglish (US)
Pages (from-to)4095-4111
Number of pages17
JournalOptics Express
Volume17
Issue number5
DOIs
StatePublished - Mar 2 2009
Externally publishedYes

Fingerprint

adaptive optics
spectral resolution
light sources
tomography
optics
broadband
fovea
nerve fibers
photoreceptors
retina
readers
bundles
dynamic range
lasers
artifacts
aberration
cones
sapphire
diodes
microscopes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Volumetric retinal imaging with ultrahigh-resolution spectral-domain optical coherence tomography and adaptive optics using two broadband light sources. / Cense, Barry; Koperda, Eric; Brown, Jeffrey M.; Kocaoglu, Omer P.; Gao, Weihua; Jonnal, Ravi; Miller, Donald T.

In: Optics Express, Vol. 17, No. 5, 02.03.2009, p. 4095-4111.

Research output: Contribution to journalArticle

Cense, Barry ; Koperda, Eric ; Brown, Jeffrey M. ; Kocaoglu, Omer P. ; Gao, Weihua ; Jonnal, Ravi ; Miller, Donald T. / Volumetric retinal imaging with ultrahigh-resolution spectral-domain optical coherence tomography and adaptive optics using two broadband light sources. In: Optics Express. 2009 ; Vol. 17, No. 5. pp. 4095-4111.
@article{c4eae6e24b62441cb11893b56245ad6a,
title = "Volumetric retinal imaging with ultrahigh-resolution spectral-domain optical coherence tomography and adaptive optics using two broadband light sources",
abstract = "Ultrabroadband sources, such as multiplexed superluminescent diodes (SLDs) and femtosecond lasers, have been successfully employed in adaptive optics optical coherence tomography (AO-OCT) systems for ultrahigh resolution retinal imaging. The large cost differential of these sources, however, motivates the need for a performance comparison. Here, we compare the performance of a Femtolasers Integral Ti: Sapphire laser and a Superlum BroadLighter T840, using the same AO-OCT system and the same subject. In addition, we investigate the capability of our instrument equipped with the Integral to capture volume images of the fovea and adjacent regions on a second subject using the AO to control focus in the retina and custom and freeware image registration software to reduce eye motion artifacts. Monochromatic ocular aberrations were corrected with a woofer-tweeter AO system. Coherence lengths of the Integral and BroadLighter were measured in vivo at 3.2 μm and 3.3 μm, respectively. The difference in dynamic range was 5 dB, close to the expected variability of the experiment. Individual cone photoreceptors, retinal capillaries and nerve fiber bundles were distinguished in all three dimensions with both sources. The acquired retinal volumes are provided for viewing in OSA ISP, allowing the reader to data mine at the microscope level.",
author = "Barry Cense and Eric Koperda and Brown, {Jeffrey M.} and Kocaoglu, {Omer P.} and Weihua Gao and Ravi Jonnal and Miller, {Donald T.}",
year = "2009",
month = "3",
day = "2",
doi = "10.1364/OE.17.004095",
language = "English (US)",
volume = "17",
pages = "4095--4111",
journal = "Optics Express",
issn = "1094-4087",
publisher = "The Optical Society",
number = "5",

}

TY - JOUR

T1 - Volumetric retinal imaging with ultrahigh-resolution spectral-domain optical coherence tomography and adaptive optics using two broadband light sources

AU - Cense, Barry

AU - Koperda, Eric

AU - Brown, Jeffrey M.

AU - Kocaoglu, Omer P.

AU - Gao, Weihua

AU - Jonnal, Ravi

AU - Miller, Donald T.

PY - 2009/3/2

Y1 - 2009/3/2

N2 - Ultrabroadband sources, such as multiplexed superluminescent diodes (SLDs) and femtosecond lasers, have been successfully employed in adaptive optics optical coherence tomography (AO-OCT) systems for ultrahigh resolution retinal imaging. The large cost differential of these sources, however, motivates the need for a performance comparison. Here, we compare the performance of a Femtolasers Integral Ti: Sapphire laser and a Superlum BroadLighter T840, using the same AO-OCT system and the same subject. In addition, we investigate the capability of our instrument equipped with the Integral to capture volume images of the fovea and adjacent regions on a second subject using the AO to control focus in the retina and custom and freeware image registration software to reduce eye motion artifacts. Monochromatic ocular aberrations were corrected with a woofer-tweeter AO system. Coherence lengths of the Integral and BroadLighter were measured in vivo at 3.2 μm and 3.3 μm, respectively. The difference in dynamic range was 5 dB, close to the expected variability of the experiment. Individual cone photoreceptors, retinal capillaries and nerve fiber bundles were distinguished in all three dimensions with both sources. The acquired retinal volumes are provided for viewing in OSA ISP, allowing the reader to data mine at the microscope level.

AB - Ultrabroadband sources, such as multiplexed superluminescent diodes (SLDs) and femtosecond lasers, have been successfully employed in adaptive optics optical coherence tomography (AO-OCT) systems for ultrahigh resolution retinal imaging. The large cost differential of these sources, however, motivates the need for a performance comparison. Here, we compare the performance of a Femtolasers Integral Ti: Sapphire laser and a Superlum BroadLighter T840, using the same AO-OCT system and the same subject. In addition, we investigate the capability of our instrument equipped with the Integral to capture volume images of the fovea and adjacent regions on a second subject using the AO to control focus in the retina and custom and freeware image registration software to reduce eye motion artifacts. Monochromatic ocular aberrations were corrected with a woofer-tweeter AO system. Coherence lengths of the Integral and BroadLighter were measured in vivo at 3.2 μm and 3.3 μm, respectively. The difference in dynamic range was 5 dB, close to the expected variability of the experiment. Individual cone photoreceptors, retinal capillaries and nerve fiber bundles were distinguished in all three dimensions with both sources. The acquired retinal volumes are provided for viewing in OSA ISP, allowing the reader to data mine at the microscope level.

UR - http://www.scopus.com/inward/record.url?scp=61549126824&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=61549126824&partnerID=8YFLogxK

U2 - 10.1364/OE.17.004095

DO - 10.1364/OE.17.004095

M3 - Article

C2 - 19259249

AN - SCOPUS:61549126824

VL - 17

SP - 4095

EP - 4111

JO - Optics Express

JF - Optics Express

SN - 1094-4087

IS - 5

ER -