Volumetric imaging of rod and cone photoreceptor structure with a combined adaptive optics-optical coherence tomography-scanning laser ophthalmoscope

Elaine M. Wells-Gray, Stacey S. Choi, Robert Zawadzki, Susanna C. Finn, Cherry Greiner, John S Werner, Nathan Doble

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We have designed and implemented a dual-mode adaptive optics (AO) imaging system that combines spectral domain optical coherence tomography (OCT) and scanning laser ophthalmoscopy (SLO) for in vivo imaging of the human retina. The system simultaneously acquires SLO frames and OCT B-scans at 60 Hz with an OCT volume acquisition time of 4.2 s. Transverse eye motion measured from the SLO is used to register the OCT B-scans to generate three-dimensional (3-D) volumes. Key optical design considerations include: minimizing system aberrations through the use of off-axis relay telescopes, conjugate pupil plane requirements, and the use of dichroic beam splitters to separate and recombine the OCT and SLO beams around the nonshared horizontal scanning mirrors. To demonstrate system performance, AO-OCT-SLO images and measurements are taken from three normal human subjects ranging in retinal eccentricity from the fovea out to 15-deg temporal and 20-deg superior. Also presented are en face OCT projections generated from the registered 3-D volumes. The ability to acquire high-resolution 3-D images of the human retina in the midperiphery and beyond has clinical importance in diseases, such as retinitis pigmentosa and cone-rod dystrophy.

Original languageEnglish (US)
Article number036003
JournalJournal of Biomedical Optics
Volume23
Issue number3
DOIs
StatePublished - Mar 1 2018

Fingerprint

photoreceptors
Adaptive optics
Optical tomography
adaptive optics
Cones
cones
rods
tomography
Scanning
Imaging techniques
scanning
Lasers
lasers
retina
fovea
Optical design
registers
relay
beam splitters
pupils

Keywords

  • 3-D imaging
  • Adaptive optics
  • Cones
  • Optical coherence tomography
  • Photoreceptors
  • Rods
  • Scanning laser ophthalmoscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Cite this

Volumetric imaging of rod and cone photoreceptor structure with a combined adaptive optics-optical coherence tomography-scanning laser ophthalmoscope. / Wells-Gray, Elaine M.; Choi, Stacey S.; Zawadzki, Robert; Finn, Susanna C.; Greiner, Cherry; Werner, John S; Doble, Nathan.

In: Journal of Biomedical Optics, Vol. 23, No. 3, 036003, 01.03.2018.

Research output: Contribution to journalArticle

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