Voltage-gated k+ channel β subunits: Expression and distribution of Kvβ1 and Kvβ2 in adult rat brain

Kenneth J. Rhodes, Michael M. Monaghan, Nestor X. Barrezueta, Stanley Nawoschik, Zewditu Bekele-Arcuri, Maria F. Matos, Kensuke Nakahira, Lee E. Schechter, James Trimmer

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

Recent cloning of K+ channel β subunits revealed that these cytoplasmic polypeptides can dramatically alter the kinetics of current inactivation and promote efficient glycosylation and surface expression of the channel-forming α subunits. Here, we examined the expression, distribution, and association of two of these β subunits, Kvβ1 and Kvβ2, in adult rat brain. In situ hybridization using cRNA probes revealed that these β-subunit genes are heterogeneously expressed, with high densities of Kvβ1 mRNA in the striatum, CA1 subfield of the hippocampus, and cerebellar Purkinje cells, and high densities of Kvβ2 mRNA in the cerebral cortex, cerebellum, and brainstem. Immunohistochemical staining using subunit- specific monoclonal and affinity-purified polyclonal antibodies revealed that the Kvβ1 and Kvβ2 polypeptides frequently co-localize and are concentrated in neuronal perikarya, dendrites, and terminal fields, and in the juxtaparanodal region of myelinated axons. Immunoblot and reciprocal co- immunoprecipitation analyses indicated that Kvβ2 is the major β subunit present in rat brain membranes, and that most K+ channel complexes containing Kvβ1 also contain Kvβ2. Taken together, these data suggest that Kvβ2 is a component of almost all K+ channel complexes containing Kv1 α subunits, and that individual channels may contain two or more biochemically and functionally distinct β-subunit polypeptides.

Original languageEnglish (US)
Pages (from-to)4846-4860
Number of pages15
JournalJournal of Neuroscience
Volume16
Issue number16
StatePublished - Aug 15 1996
Externally publishedYes

Fingerprint

Peptides
Brain
Complementary RNA
Messenger RNA
Purkinje Cells
Dendrites
Glycosylation
Immunoprecipitation
Cerebral Cortex
Cerebellum
Brain Stem
In Situ Hybridization
Axons
Organism Cloning
Hippocampus
Cell Count
Staining and Labeling
Membranes
Antibodies
Genes

Keywords

  • auxiliary subunit
  • central nervous system
  • immunohistochemistry
  • immunoprecipitation
  • ion channel
  • striatum

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Rhodes, K. J., Monaghan, M. M., Barrezueta, N. X., Nawoschik, S., Bekele-Arcuri, Z., Matos, M. F., ... Trimmer, J. (1996). Voltage-gated k+ channel β subunits: Expression and distribution of Kvβ1 and Kvβ2 in adult rat brain. Journal of Neuroscience, 16(16), 4846-4860.

Voltage-gated k+ channel β subunits : Expression and distribution of Kvβ1 and Kvβ2 in adult rat brain. / Rhodes, Kenneth J.; Monaghan, Michael M.; Barrezueta, Nestor X.; Nawoschik, Stanley; Bekele-Arcuri, Zewditu; Matos, Maria F.; Nakahira, Kensuke; Schechter, Lee E.; Trimmer, James.

In: Journal of Neuroscience, Vol. 16, No. 16, 15.08.1996, p. 4846-4860.

Research output: Contribution to journalArticle

Rhodes, KJ, Monaghan, MM, Barrezueta, NX, Nawoschik, S, Bekele-Arcuri, Z, Matos, MF, Nakahira, K, Schechter, LE & Trimmer, J 1996, 'Voltage-gated k+ channel β subunits: Expression and distribution of Kvβ1 and Kvβ2 in adult rat brain', Journal of Neuroscience, vol. 16, no. 16, pp. 4846-4860.
Rhodes KJ, Monaghan MM, Barrezueta NX, Nawoschik S, Bekele-Arcuri Z, Matos MF et al. Voltage-gated k+ channel β subunits: Expression and distribution of Kvβ1 and Kvβ2 in adult rat brain. Journal of Neuroscience. 1996 Aug 15;16(16):4846-4860.
Rhodes, Kenneth J. ; Monaghan, Michael M. ; Barrezueta, Nestor X. ; Nawoschik, Stanley ; Bekele-Arcuri, Zewditu ; Matos, Maria F. ; Nakahira, Kensuke ; Schechter, Lee E. ; Trimmer, James. / Voltage-gated k+ channel β subunits : Expression and distribution of Kvβ1 and Kvβ2 in adult rat brain. In: Journal of Neuroscience. 1996 ; Vol. 16, No. 16. pp. 4846-4860.
@article{17b1e570df8a4f28936dea39c8e23717,
title = "Voltage-gated k+ channel β subunits: Expression and distribution of Kvβ1 and Kvβ2 in adult rat brain",
abstract = "Recent cloning of K+ channel β subunits revealed that these cytoplasmic polypeptides can dramatically alter the kinetics of current inactivation and promote efficient glycosylation and surface expression of the channel-forming α subunits. Here, we examined the expression, distribution, and association of two of these β subunits, Kvβ1 and Kvβ2, in adult rat brain. In situ hybridization using cRNA probes revealed that these β-subunit genes are heterogeneously expressed, with high densities of Kvβ1 mRNA in the striatum, CA1 subfield of the hippocampus, and cerebellar Purkinje cells, and high densities of Kvβ2 mRNA in the cerebral cortex, cerebellum, and brainstem. Immunohistochemical staining using subunit- specific monoclonal and affinity-purified polyclonal antibodies revealed that the Kvβ1 and Kvβ2 polypeptides frequently co-localize and are concentrated in neuronal perikarya, dendrites, and terminal fields, and in the juxtaparanodal region of myelinated axons. Immunoblot and reciprocal co- immunoprecipitation analyses indicated that Kvβ2 is the major β subunit present in rat brain membranes, and that most K+ channel complexes containing Kvβ1 also contain Kvβ2. Taken together, these data suggest that Kvβ2 is a component of almost all K+ channel complexes containing Kv1 α subunits, and that individual channels may contain two or more biochemically and functionally distinct β-subunit polypeptides.",
keywords = "auxiliary subunit, central nervous system, immunohistochemistry, immunoprecipitation, ion channel, striatum",
author = "Rhodes, {Kenneth J.} and Monaghan, {Michael M.} and Barrezueta, {Nestor X.} and Stanley Nawoschik and Zewditu Bekele-Arcuri and Matos, {Maria F.} and Kensuke Nakahira and Schechter, {Lee E.} and James Trimmer",
year = "1996",
month = "8",
day = "15",
language = "English (US)",
volume = "16",
pages = "4846--4860",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "16",

}

TY - JOUR

T1 - Voltage-gated k+ channel β subunits

T2 - Expression and distribution of Kvβ1 and Kvβ2 in adult rat brain

AU - Rhodes, Kenneth J.

AU - Monaghan, Michael M.

AU - Barrezueta, Nestor X.

AU - Nawoschik, Stanley

AU - Bekele-Arcuri, Zewditu

AU - Matos, Maria F.

AU - Nakahira, Kensuke

AU - Schechter, Lee E.

AU - Trimmer, James

PY - 1996/8/15

Y1 - 1996/8/15

N2 - Recent cloning of K+ channel β subunits revealed that these cytoplasmic polypeptides can dramatically alter the kinetics of current inactivation and promote efficient glycosylation and surface expression of the channel-forming α subunits. Here, we examined the expression, distribution, and association of two of these β subunits, Kvβ1 and Kvβ2, in adult rat brain. In situ hybridization using cRNA probes revealed that these β-subunit genes are heterogeneously expressed, with high densities of Kvβ1 mRNA in the striatum, CA1 subfield of the hippocampus, and cerebellar Purkinje cells, and high densities of Kvβ2 mRNA in the cerebral cortex, cerebellum, and brainstem. Immunohistochemical staining using subunit- specific monoclonal and affinity-purified polyclonal antibodies revealed that the Kvβ1 and Kvβ2 polypeptides frequently co-localize and are concentrated in neuronal perikarya, dendrites, and terminal fields, and in the juxtaparanodal region of myelinated axons. Immunoblot and reciprocal co- immunoprecipitation analyses indicated that Kvβ2 is the major β subunit present in rat brain membranes, and that most K+ channel complexes containing Kvβ1 also contain Kvβ2. Taken together, these data suggest that Kvβ2 is a component of almost all K+ channel complexes containing Kv1 α subunits, and that individual channels may contain two or more biochemically and functionally distinct β-subunit polypeptides.

AB - Recent cloning of K+ channel β subunits revealed that these cytoplasmic polypeptides can dramatically alter the kinetics of current inactivation and promote efficient glycosylation and surface expression of the channel-forming α subunits. Here, we examined the expression, distribution, and association of two of these β subunits, Kvβ1 and Kvβ2, in adult rat brain. In situ hybridization using cRNA probes revealed that these β-subunit genes are heterogeneously expressed, with high densities of Kvβ1 mRNA in the striatum, CA1 subfield of the hippocampus, and cerebellar Purkinje cells, and high densities of Kvβ2 mRNA in the cerebral cortex, cerebellum, and brainstem. Immunohistochemical staining using subunit- specific monoclonal and affinity-purified polyclonal antibodies revealed that the Kvβ1 and Kvβ2 polypeptides frequently co-localize and are concentrated in neuronal perikarya, dendrites, and terminal fields, and in the juxtaparanodal region of myelinated axons. Immunoblot and reciprocal co- immunoprecipitation analyses indicated that Kvβ2 is the major β subunit present in rat brain membranes, and that most K+ channel complexes containing Kvβ1 also contain Kvβ2. Taken together, these data suggest that Kvβ2 is a component of almost all K+ channel complexes containing Kv1 α subunits, and that individual channels may contain two or more biochemically and functionally distinct β-subunit polypeptides.

KW - auxiliary subunit

KW - central nervous system

KW - immunohistochemistry

KW - immunoprecipitation

KW - ion channel

KW - striatum

UR - http://www.scopus.com/inward/record.url?scp=0029738466&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029738466&partnerID=8YFLogxK

M3 - Article

C2 - 8756417

AN - SCOPUS:0029738466

VL - 16

SP - 4846

EP - 4860

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 16

ER -