Vitronectin receptors are expressed by macaque trophoblast cells and play a role in migration and adhesion to endothelium

Gordon C Douglas, Twanda L. Thirkill, Thomas N. Blankenship

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The objective of this work was to develop an in vitro system that would extend the usefulness of the macaque as a model for studying trophoblast invasion and spiral artery modification. We sought to determine whether trophoblast cells isolated from early gestation macaque placentas expressed vitronectin receptors and tested the idea that these receptors play a role in trophoblast migration and adhesion. Cytotrophoblast cells were isolated from 40-100 day macaque placentas, cultured, and characterized by immunofluorescence microscopy and flow cytometry. The cells expressed αV, β3, and β1 integrins on their surfaces. Immunohistochemical analysis of early gestation placentas and decidua basalis confirmed that intravascular trophoblast cells express αVβ3/β5. Using migration chambers we found that the trophoblast cells migrated towards vitronectin but not towards bovine serum albumin. This specific migration was blocked by preincubating the trophoblast cells with anti-vitronectin receptor (αVβ3/β5) antibodies. In other experiments, macaque trophoblast cells adhered to myometrial endothelial cells in a time-dependent manner and adhesion was significantly blocked by antibodies against αVβ3/β5 integrin. The results suggest that vitronectin receptors expressed by macaque trophoblast cells play a role in the migratory activity of these cells and may also be important in mediating attachment to endothelium.

Original languageEnglish (US)
Pages (from-to)36-45
Number of pages10
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1452
Issue number1
DOIs
StatePublished - Oct 13 1999

Fingerprint

Vitronectin Receptors
Trophoblasts
Macaca
Endothelium
Placenta
Integrins
Integrin alphaVbeta3
Vitronectin
Decidua
Pregnancy
Antibodies
Bovine Serum Albumin
Fluorescence Microscopy
Flow Cytometry

Keywords

  • Adhesion
  • Endothelium
  • Integrin
  • Invasion
  • Trophoblast
  • Vitronectin receptor

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Biophysics

Cite this

Vitronectin receptors are expressed by macaque trophoblast cells and play a role in migration and adhesion to endothelium. / Douglas, Gordon C; Thirkill, Twanda L.; Blankenship, Thomas N.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1452, No. 1, 13.10.1999, p. 36-45.

Research output: Contribution to journalArticle

@article{f9c57ea0a8a346cb8431a24994c25d3a,
title = "Vitronectin receptors are expressed by macaque trophoblast cells and play a role in migration and adhesion to endothelium",
abstract = "The objective of this work was to develop an in vitro system that would extend the usefulness of the macaque as a model for studying trophoblast invasion and spiral artery modification. We sought to determine whether trophoblast cells isolated from early gestation macaque placentas expressed vitronectin receptors and tested the idea that these receptors play a role in trophoblast migration and adhesion. Cytotrophoblast cells were isolated from 40-100 day macaque placentas, cultured, and characterized by immunofluorescence microscopy and flow cytometry. The cells expressed αV, β3, and β1 integrins on their surfaces. Immunohistochemical analysis of early gestation placentas and decidua basalis confirmed that intravascular trophoblast cells express αVβ3/β5. Using migration chambers we found that the trophoblast cells migrated towards vitronectin but not towards bovine serum albumin. This specific migration was blocked by preincubating the trophoblast cells with anti-vitronectin receptor (αVβ3/β5) antibodies. In other experiments, macaque trophoblast cells adhered to myometrial endothelial cells in a time-dependent manner and adhesion was significantly blocked by antibodies against αVβ3/β5 integrin. The results suggest that vitronectin receptors expressed by macaque trophoblast cells play a role in the migratory activity of these cells and may also be important in mediating attachment to endothelium.",
keywords = "Adhesion, Endothelium, Integrin, Invasion, Trophoblast, Vitronectin receptor",
author = "Douglas, {Gordon C} and Thirkill, {Twanda L.} and Blankenship, {Thomas N.}",
year = "1999",
month = "10",
day = "13",
doi = "10.1016/S0167-4889(99)00109-3",
language = "English (US)",
volume = "1452",
pages = "36--45",
journal = "Biochimica et Biophysica Acta - Molecular Cell Research",
issn = "0167-4889",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Vitronectin receptors are expressed by macaque trophoblast cells and play a role in migration and adhesion to endothelium

AU - Douglas, Gordon C

AU - Thirkill, Twanda L.

AU - Blankenship, Thomas N.

PY - 1999/10/13

Y1 - 1999/10/13

N2 - The objective of this work was to develop an in vitro system that would extend the usefulness of the macaque as a model for studying trophoblast invasion and spiral artery modification. We sought to determine whether trophoblast cells isolated from early gestation macaque placentas expressed vitronectin receptors and tested the idea that these receptors play a role in trophoblast migration and adhesion. Cytotrophoblast cells were isolated from 40-100 day macaque placentas, cultured, and characterized by immunofluorescence microscopy and flow cytometry. The cells expressed αV, β3, and β1 integrins on their surfaces. Immunohistochemical analysis of early gestation placentas and decidua basalis confirmed that intravascular trophoblast cells express αVβ3/β5. Using migration chambers we found that the trophoblast cells migrated towards vitronectin but not towards bovine serum albumin. This specific migration was blocked by preincubating the trophoblast cells with anti-vitronectin receptor (αVβ3/β5) antibodies. In other experiments, macaque trophoblast cells adhered to myometrial endothelial cells in a time-dependent manner and adhesion was significantly blocked by antibodies against αVβ3/β5 integrin. The results suggest that vitronectin receptors expressed by macaque trophoblast cells play a role in the migratory activity of these cells and may also be important in mediating attachment to endothelium.

AB - The objective of this work was to develop an in vitro system that would extend the usefulness of the macaque as a model for studying trophoblast invasion and spiral artery modification. We sought to determine whether trophoblast cells isolated from early gestation macaque placentas expressed vitronectin receptors and tested the idea that these receptors play a role in trophoblast migration and adhesion. Cytotrophoblast cells were isolated from 40-100 day macaque placentas, cultured, and characterized by immunofluorescence microscopy and flow cytometry. The cells expressed αV, β3, and β1 integrins on their surfaces. Immunohistochemical analysis of early gestation placentas and decidua basalis confirmed that intravascular trophoblast cells express αVβ3/β5. Using migration chambers we found that the trophoblast cells migrated towards vitronectin but not towards bovine serum albumin. This specific migration was blocked by preincubating the trophoblast cells with anti-vitronectin receptor (αVβ3/β5) antibodies. In other experiments, macaque trophoblast cells adhered to myometrial endothelial cells in a time-dependent manner and adhesion was significantly blocked by antibodies against αVβ3/β5 integrin. The results suggest that vitronectin receptors expressed by macaque trophoblast cells play a role in the migratory activity of these cells and may also be important in mediating attachment to endothelium.

KW - Adhesion

KW - Endothelium

KW - Integrin

KW - Invasion

KW - Trophoblast

KW - Vitronectin receptor

UR - http://www.scopus.com/inward/record.url?scp=0032853930&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032853930&partnerID=8YFLogxK

U2 - 10.1016/S0167-4889(99)00109-3

DO - 10.1016/S0167-4889(99)00109-3

M3 - Article

C2 - 10525158

AN - SCOPUS:0032853930

VL - 1452

SP - 36

EP - 45

JO - Biochimica et Biophysica Acta - Molecular Cell Research

JF - Biochimica et Biophysica Acta - Molecular Cell Research

SN - 0167-4889

IS - 1

ER -