Vitrification of equine oocytes with a polyvinyl alcohol after in vitro maturation with equine growth hormone and insulin-like growth factor-i

Bruna Da Rosa Curcio, Gabriel Ribas Pereira, Lucas Insaurriaga Antunes, André Nunes Boff, Fernanda Carlini Cunha Dos Santos, Thomaz Lucia, Carlos Eduardo Wayne Nogueira, Carine Dahl Corcini, Irwin Liu, João Carlos Deschamps

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

BACKGROUND: In vitro fertilization (IVF) procedures are limited by the inability to mature equine oocytes on in vitro methods. OBJECTIVE: The aim of this study was to evaluate structural integrity of equine oocytes subjected to vitrification with a synthetic polymer (PVA). METHODS: The effect of eGH and its relationship with IGF-I on in vitro maturation (IVM) were evaluated. Compact cumulus oocytes complexes (n=122) were cultured in TCM-199 with eGH, IGF-I or eGH+IGF-I for 30h at 38.5C in air with 5% CO2. Oocytes were fixed after IVM or subjected to the vitrification protocol. Cryopreserved oocytes were exposed to 1.4M DMS0+1.8M EG+1% PVA for 3min, and then transfer to 2.8M DMSO+3.6M EG+0,6M sucrose+1% PVA for lmin. After rewarming, oocytes were evaluated by confocal microscopy. RESULTS: Maturation rates of oocytes were not significant different among groups (P>0.05), however eGH+IGF-I group can develop the assessment of resumption of meiosis (MI+MII = 86.7%). CONCLUSION: The oocyte did not show morphological alterations. The use of PVA-copolymer may represent a potential alternative for vitrification of equine oocytes after IVM.

Original languageEnglish (US)
Pages (from-to)90-94
Number of pages5
JournalCryo-Letters
Volume35
Issue number2
StatePublished - Jan 1 2014

Fingerprint

Vitrification
Polyvinyl Alcohol
polyvinyl alcohol
vitrification
somatomedins
Somatomedins
somatotropin
Growth Hormone
Horses
Oocytes
oocytes
horses
Insulin-Like Growth Factor I
insulin-like growth factor I
In Vitro Oocyte Maturation Techniques
Rewarming
In Vitro Techniques
Meiosis
Fertilization in Vitro
composite polymers

Keywords

  • Horses
  • In vitro maturation
  • Oocyte cryopreservation
  • PVA copolymer

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Anatomy

Cite this

Da Rosa Curcio, B., Pereira, G. R., Antunes, L. I., Boff, A. N., Dos Santos, F. C. C., Lucia, T., ... Deschamps, J. C. (2014). Vitrification of equine oocytes with a polyvinyl alcohol after in vitro maturation with equine growth hormone and insulin-like growth factor-i. Cryo-Letters, 35(2), 90-94.

Vitrification of equine oocytes with a polyvinyl alcohol after in vitro maturation with equine growth hormone and insulin-like growth factor-i. / Da Rosa Curcio, Bruna; Pereira, Gabriel Ribas; Antunes, Lucas Insaurriaga; Boff, André Nunes; Dos Santos, Fernanda Carlini Cunha; Lucia, Thomaz; Nogueira, Carlos Eduardo Wayne; Corcini, Carine Dahl; Liu, Irwin; Deschamps, João Carlos.

In: Cryo-Letters, Vol. 35, No. 2, 01.01.2014, p. 90-94.

Research output: Contribution to journalArticle

Da Rosa Curcio, B, Pereira, GR, Antunes, LI, Boff, AN, Dos Santos, FCC, Lucia, T, Nogueira, CEW, Corcini, CD, Liu, I & Deschamps, JC 2014, 'Vitrification of equine oocytes with a polyvinyl alcohol after in vitro maturation with equine growth hormone and insulin-like growth factor-i', Cryo-Letters, vol. 35, no. 2, pp. 90-94.
Da Rosa Curcio B, Pereira GR, Antunes LI, Boff AN, Dos Santos FCC, Lucia T et al. Vitrification of equine oocytes with a polyvinyl alcohol after in vitro maturation with equine growth hormone and insulin-like growth factor-i. Cryo-Letters. 2014 Jan 1;35(2):90-94.
Da Rosa Curcio, Bruna ; Pereira, Gabriel Ribas ; Antunes, Lucas Insaurriaga ; Boff, André Nunes ; Dos Santos, Fernanda Carlini Cunha ; Lucia, Thomaz ; Nogueira, Carlos Eduardo Wayne ; Corcini, Carine Dahl ; Liu, Irwin ; Deschamps, João Carlos. / Vitrification of equine oocytes with a polyvinyl alcohol after in vitro maturation with equine growth hormone and insulin-like growth factor-i. In: Cryo-Letters. 2014 ; Vol. 35, No. 2. pp. 90-94.
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abstract = "BACKGROUND: In vitro fertilization (IVF) procedures are limited by the inability to mature equine oocytes on in vitro methods. OBJECTIVE: The aim of this study was to evaluate structural integrity of equine oocytes subjected to vitrification with a synthetic polymer (PVA). METHODS: The effect of eGH and its relationship with IGF-I on in vitro maturation (IVM) were evaluated. Compact cumulus oocytes complexes (n=122) were cultured in TCM-199 with eGH, IGF-I or eGH+IGF-I for 30h at 38.5C in air with 5{\%} CO2. Oocytes were fixed after IVM or subjected to the vitrification protocol. Cryopreserved oocytes were exposed to 1.4M DMS0+1.8M EG+1{\%} PVA for 3min, and then transfer to 2.8M DMSO+3.6M EG+0,6M sucrose+1{\%} PVA for lmin. After rewarming, oocytes were evaluated by confocal microscopy. RESULTS: Maturation rates of oocytes were not significant different among groups (P>0.05), however eGH+IGF-I group can develop the assessment of resumption of meiosis (MI+MII = 86.7{\%}). CONCLUSION: The oocyte did not show morphological alterations. The use of PVA-copolymer may represent a potential alternative for vitrification of equine oocytes after IVM.",
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AU - Boff, André Nunes

AU - Dos Santos, Fernanda Carlini Cunha

AU - Lucia, Thomaz

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AB - BACKGROUND: In vitro fertilization (IVF) procedures are limited by the inability to mature equine oocytes on in vitro methods. OBJECTIVE: The aim of this study was to evaluate structural integrity of equine oocytes subjected to vitrification with a synthetic polymer (PVA). METHODS: The effect of eGH and its relationship with IGF-I on in vitro maturation (IVM) were evaluated. Compact cumulus oocytes complexes (n=122) were cultured in TCM-199 with eGH, IGF-I or eGH+IGF-I for 30h at 38.5C in air with 5% CO2. Oocytes were fixed after IVM or subjected to the vitrification protocol. Cryopreserved oocytes were exposed to 1.4M DMS0+1.8M EG+1% PVA for 3min, and then transfer to 2.8M DMSO+3.6M EG+0,6M sucrose+1% PVA for lmin. After rewarming, oocytes were evaluated by confocal microscopy. RESULTS: Maturation rates of oocytes were not significant different among groups (P>0.05), however eGH+IGF-I group can develop the assessment of resumption of meiosis (MI+MII = 86.7%). CONCLUSION: The oocyte did not show morphological alterations. The use of PVA-copolymer may represent a potential alternative for vitrification of equine oocytes after IVM.

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