Vitelline envelope formation during oogenesis in Bufo arenarum

M. O. Cabada, A. N. Sánchez Riera, H. D. Genta, S. S. Sánchez, Gustavo Barisone

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The formation of vitelline envelope (VE) during the oogenesis of Bufo arenarum (Amphibia Anura) is described. At the stage of early vitellogenesis, the first structures appear: the number of oocyte microvilli increases, and many cross sections of them are observed between the follicle cells and the oocyte. A filamentous material is observed inside the follicle cells and between the follicle cells and the oocyte. Multivesicular bodies are also found in the follicle cells, and in the perivitelline space. The micrographs also suggest the participation of the oocyte in the process of VE formation: large vesicles are present in the cortex of the oocyte, filled with an amorphous material of low and uniform electron density. Some of them are in the process of releasing their content to the perivitelline space. Many vesicles (probably resulting from microvilli fragmentation) are also observed in the perivitelline space. During late vitellogenesis the VE is a continuous structure between the layer of follicle cells and the oocyte. The filamentous material is aggregated in bundles, forming a net, and the spherical components are now either included in the orifices of the net, or free near the oocyte's surface. At the end of oogenesis, when the VE is completely formed, it is difficult to distinguish its components independently. Immunolocalization with antibodies against VE, show a positive reaction in follicle cells and oocytes in previtellogenic and full grown ovarian follicles. This analysis suggests that both the oocyte and the follicle cells are directly involved in the synthesis and secretion of the components of the vitelline envelope in Bufo arenarum.

Original languageEnglish (US)
Pages (from-to)77-86
Number of pages10
JournalBiocell
Volume20
Issue number1
StatePublished - Dec 1 1996
Externally publishedYes

Fingerprint

Bufo arenarum
Oogenesis
Oocytes
Vitellogenesis
Microvilli
Multivesicular Bodies
Ovarian Follicle
Amphibians
Anura

Keywords

  • Bufo arenarum
  • Follicle cells
  • Vitelline envelope

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cabada, M. O., Sánchez Riera, A. N., Genta, H. D., Sánchez, S. S., & Barisone, G. (1996). Vitelline envelope formation during oogenesis in Bufo arenarum. Biocell, 20(1), 77-86.

Vitelline envelope formation during oogenesis in Bufo arenarum. / Cabada, M. O.; Sánchez Riera, A. N.; Genta, H. D.; Sánchez, S. S.; Barisone, Gustavo.

In: Biocell, Vol. 20, No. 1, 01.12.1996, p. 77-86.

Research output: Contribution to journalArticle

Cabada, MO, Sánchez Riera, AN, Genta, HD, Sánchez, SS & Barisone, G 1996, 'Vitelline envelope formation during oogenesis in Bufo arenarum', Biocell, vol. 20, no. 1, pp. 77-86.
Cabada MO, Sánchez Riera AN, Genta HD, Sánchez SS, Barisone G. Vitelline envelope formation during oogenesis in Bufo arenarum. Biocell. 1996 Dec 1;20(1):77-86.
Cabada, M. O. ; Sánchez Riera, A. N. ; Genta, H. D. ; Sánchez, S. S. ; Barisone, Gustavo. / Vitelline envelope formation during oogenesis in Bufo arenarum. In: Biocell. 1996 ; Vol. 20, No. 1. pp. 77-86.
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