Vitamin C, uric acid, and glutathione gradients in murine stratum corneum and their susceptibility to ozone exposure

Stefan Udo Weber, Jens J. Thiele, Carroll E Cross, Lester Packer

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

The stratum corneum has been recognized as the main cutaneous oxidation target of atmospheric ozone (O3), a major part of photochemical smog. This study reports the presence and distribution of vitamin C, glutathione, and uric acid in murine stratum corneum, and evaluates their susceptibility to acute environmental exposure to O3. Based on tape stripping and a modified extraction method with high performance liquid chromatography electrochemical analysis, we detected vitamin C (208.0 ± 82.5 pmol per 10 consecutive pooled tapes), glutathione (283.7 ± 96.3), and uric acid (286.4 ± 47.1) in murine stratum corneum as compared with only 16.5 ± 1.4 pmol α-tocopherol. Vitamin C, glutathione (both p < 00.001), and urate (p < 0.01) were found to exhibit a gradient with the lowest concentrations in the outer layers and a steep increase in the deeper layers. To investigate the effect of O3 exposure on hydrophilic antioxidants, we exposed SKH-1 hairless mice to O3 concentrations of 0, 0.8, 1, and 10 p.p.m., and stratum corneum was analyzed before and after exposure. Whereas mock exposure with 0 p.p.m, for 2 h had no significant effect, O3 doses of 1 p.p.m. for 2 h and above showed depletion of all three antioxidants. Vitamin C was decreased to 80% ± 15% of its pretreatment content (p<0.05), GSH to 41% ± 24% (p < 0.01), and uric acid to 44% ± 28% (p < 0.01). This report demonstrates the previously unrecognized role of hydrophilic antioxidants in the stratum corneum and provides further evidence that O3 induces oxidative stress in this outer skin layer.

Original languageEnglish (US)
Pages (from-to)1128-1132
Number of pages5
JournalJournal of Investigative Dermatology
Volume113
Issue number6
DOIs
StatePublished - 1999

Fingerprint

Ozone
Uric Acid
Cornea
Ascorbic Acid
Glutathione
Antioxidants
Tapes
Smog
Hairless Mouse
Skin
Oxidative stress
Tocopherols
Environmental Exposure
High performance liquid chromatography
Oxidative Stress
High Pressure Liquid Chromatography
Oxidation

Keywords

  • Antioxidant
  • Oxidative stress
  • Skin
  • Skin barrier
  • Vitamin E

ASJC Scopus subject areas

  • Dermatology

Cite this

Vitamin C, uric acid, and glutathione gradients in murine stratum corneum and their susceptibility to ozone exposure. / Weber, Stefan Udo; Thiele, Jens J.; Cross, Carroll E; Packer, Lester.

In: Journal of Investigative Dermatology, Vol. 113, No. 6, 1999, p. 1128-1132.

Research output: Contribution to journalArticle

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