Vitamin B-12 treatment of asymptomatic, deficient, elderly Chileans improves conductivity in myelinated peripheral nerves, but high serum folate impairs Vitamin B-12 status response assessed by the combined indicator of Vitamin B-12 status

Alex Brito, Renato Verdugo, Eva Hertrampf, Joshua W. Miller, Ralph Green, Sergey N. Fedosov, Setareh Shahab-Ferdows, Hugo Sanchez, Cecilia Albala, Jose L. Castillo, Jose M. Matamala, Ricardo Uauy, Lindsay H. Allen

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Background: It is uncertain whether vitamin B-12 supplementation can improve neurophysiologic function in asymptomatic elderly with low vitamin B-12 status or whether folate status affects responses to vitamin B-12 supplementation. Objective: We assessed the effects of a single intramuscular injection of 10 mg vitamin B-12 (which also contained 100 mg vitamin B-6 and 100 mg vitamin B-1) on vitamin B-12 status and neurophysiologic function in elderly community-dwelling Chileans with low serum vitamin B-12 concentrations who were consuming bread fortified with folic acid. Design: A pretreatment and posttreatment study was conducted in 51 participants (median ± SD age: 73 ± 3 y; women: 47%) with serum vitamin B-12 concentrations <120 pmol/L at screening. Vitamin B-12 status was defined by combining vitamin B-12, plasma total homocysteine (tHcy), methylmalonic acid (MMA), and holotranscobalamin into one variable [combined indicator of vitamin B-12 status (cB-12)]. The response to treatment was assessed by measuring cB-12 and neurophysiologic variables at baseline and 4 mo after treatment. Results: Treatment increased serum vitamin B-12, holotranscobalamin, and cB-12 (P <0.001) and reduced plasma tHcy and serum MMA (P <0.001). Treatment produced consistent improvements in conduction in myelinated peripheral nerves; the sensory latency of both the left and right sural nerves improved on the basis of faster median conduction times of 3.1 and 3.0 ms and 3.3 and 3.4 ms, respectively (P <0.0001). A total of 10 sensory potentials were newly observed in sural nerves after treatment. Participants with high serum folate at baseline (above the median, ≤33.9 nmol/L) had less improvement in cB-12 (P <0.001) than did individuals whose serum folate was less than the median concentration (i.e., with a concentration

Original languageEnglish (US)
Pages (from-to)250-257
Number of pages8
JournalAmerican Journal of Clinical Nutrition
Volume103
Issue number1
DOIs
StatePublished - Jan 1 2016

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Vitamin B 12
Peripheral Nerves
Folic Acid
Serum
Methylmalonic Acid
Therapeutics
Sural Nerve
Homocysteine
Independent Living
Vitamin B 6
Thiamine
Bread
Intramuscular Injections

Keywords

  • Chile
  • Elderly
  • Folate
  • Folic acid fortification
  • Holotranscobalamin
  • Methylmalonic acid
  • Nerve conductivity
  • Total homocysteine
  • Vitamin B-12

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Vitamin B-12 treatment of asymptomatic, deficient, elderly Chileans improves conductivity in myelinated peripheral nerves, but high serum folate impairs Vitamin B-12 status response assessed by the combined indicator of Vitamin B-12 status. / Brito, Alex; Verdugo, Renato; Hertrampf, Eva; Miller, Joshua W.; Green, Ralph; Fedosov, Sergey N.; Shahab-Ferdows, Setareh; Sanchez, Hugo; Albala, Cecilia; Castillo, Jose L.; Matamala, Jose M.; Uauy, Ricardo; Allen, Lindsay H.

In: American Journal of Clinical Nutrition, Vol. 103, No. 1, 01.01.2016, p. 250-257.

Research output: Contribution to journalArticle

Brito, Alex ; Verdugo, Renato ; Hertrampf, Eva ; Miller, Joshua W. ; Green, Ralph ; Fedosov, Sergey N. ; Shahab-Ferdows, Setareh ; Sanchez, Hugo ; Albala, Cecilia ; Castillo, Jose L. ; Matamala, Jose M. ; Uauy, Ricardo ; Allen, Lindsay H. / Vitamin B-12 treatment of asymptomatic, deficient, elderly Chileans improves conductivity in myelinated peripheral nerves, but high serum folate impairs Vitamin B-12 status response assessed by the combined indicator of Vitamin B-12 status. In: American Journal of Clinical Nutrition. 2016 ; Vol. 103, No. 1. pp. 250-257.
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abstract = "Background: It is uncertain whether vitamin B-12 supplementation can improve neurophysiologic function in asymptomatic elderly with low vitamin B-12 status or whether folate status affects responses to vitamin B-12 supplementation. Objective: We assessed the effects of a single intramuscular injection of 10 mg vitamin B-12 (which also contained 100 mg vitamin B-6 and 100 mg vitamin B-1) on vitamin B-12 status and neurophysiologic function in elderly community-dwelling Chileans with low serum vitamin B-12 concentrations who were consuming bread fortified with folic acid. Design: A pretreatment and posttreatment study was conducted in 51 participants (median ± SD age: 73 ± 3 y; women: 47{\%}) with serum vitamin B-12 concentrations <120 pmol/L at screening. Vitamin B-12 status was defined by combining vitamin B-12, plasma total homocysteine (tHcy), methylmalonic acid (MMA), and holotranscobalamin into one variable [combined indicator of vitamin B-12 status (cB-12)]. The response to treatment was assessed by measuring cB-12 and neurophysiologic variables at baseline and 4 mo after treatment. Results: Treatment increased serum vitamin B-12, holotranscobalamin, and cB-12 (P <0.001) and reduced plasma tHcy and serum MMA (P <0.001). Treatment produced consistent improvements in conduction in myelinated peripheral nerves; the sensory latency of both the left and right sural nerves improved on the basis of faster median conduction times of 3.1 and 3.0 ms and 3.3 and 3.4 ms, respectively (P <0.0001). A total of 10 sensory potentials were newly observed in sural nerves after treatment. Participants with high serum folate at baseline (above the median, ≤33.9 nmol/L) had less improvement in cB-12 (P <0.001) than did individuals whose serum folate was less than the median concentration (i.e., with a concentration",
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AU - Verdugo, Renato

AU - Hertrampf, Eva

AU - Miller, Joshua W.

AU - Green, Ralph

AU - Fedosov, Sergey N.

AU - Shahab-Ferdows, Setareh

AU - Sanchez, Hugo

AU - Albala, Cecilia

AU - Castillo, Jose L.

AU - Matamala, Jose M.

AU - Uauy, Ricardo

AU - Allen, Lindsay H.

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N2 - Background: It is uncertain whether vitamin B-12 supplementation can improve neurophysiologic function in asymptomatic elderly with low vitamin B-12 status or whether folate status affects responses to vitamin B-12 supplementation. Objective: We assessed the effects of a single intramuscular injection of 10 mg vitamin B-12 (which also contained 100 mg vitamin B-6 and 100 mg vitamin B-1) on vitamin B-12 status and neurophysiologic function in elderly community-dwelling Chileans with low serum vitamin B-12 concentrations who were consuming bread fortified with folic acid. Design: A pretreatment and posttreatment study was conducted in 51 participants (median ± SD age: 73 ± 3 y; women: 47%) with serum vitamin B-12 concentrations <120 pmol/L at screening. Vitamin B-12 status was defined by combining vitamin B-12, plasma total homocysteine (tHcy), methylmalonic acid (MMA), and holotranscobalamin into one variable [combined indicator of vitamin B-12 status (cB-12)]. The response to treatment was assessed by measuring cB-12 and neurophysiologic variables at baseline and 4 mo after treatment. Results: Treatment increased serum vitamin B-12, holotranscobalamin, and cB-12 (P <0.001) and reduced plasma tHcy and serum MMA (P <0.001). Treatment produced consistent improvements in conduction in myelinated peripheral nerves; the sensory latency of both the left and right sural nerves improved on the basis of faster median conduction times of 3.1 and 3.0 ms and 3.3 and 3.4 ms, respectively (P <0.0001). A total of 10 sensory potentials were newly observed in sural nerves after treatment. Participants with high serum folate at baseline (above the median, ≤33.9 nmol/L) had less improvement in cB-12 (P <0.001) than did individuals whose serum folate was less than the median concentration (i.e., with a concentration

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