Abstract
The secretory immunoglobulin A (IgA) antibody response to infections of mucosal surfaces requires transport of IgA from the basal to apical surface of mucosal epithelial cells by a specific transport protein, the polymeric immunoglobulin receptor (plgR). We have tested the hypothesis that the vitamin A metabolite all-trans retinoic acid (RA) is required for the regulation of plgR expression by the cytokines interleukin-4 (IL-4) and interferon γ (IFN-γ) in HT-29 cells, a well-differentiated human epithelial cell line derived from a colonic carcinoma. plgR expression is upregulated by IFN-γ and IL-4 when HT-29 cells are grown in normal media, but this upregulation was significantly lower when cells were grown in vitamin A- depleted media. Treatment with RA at concentrations from 10-9 to 10-5 mol/L restored normal levels of plgR expression. The percentages of cells expressing cell-surface plgR after 24, 48 and 72 h of treatment with RA, IL- 4 and IFN-γ were 66 ± 10, 90 ± 5 and 92 ± 1, respectively, significantly higher than the percentages seen without RA treatment, which were 32 ± 2.3, 72 ± 1.2 and 30 ± 7, respectively. In addition, the intensity of fluorescence of plgR-positive cells was significantly higher in the RA- treated cultures than in the cultures without RA treatment. Similarly, plgR mRNA levels (adjusted for β-actin mRNA levels) in RA-supplemented cultures were 404, 105 and 949% higher at 24, 48 and 72 h, respectively, than were plgR mRNA levels in identical cultures grown in the absence of RA. These data indicate that RA strongly interacts with IL-4 and IFN-γ to regulate plgR expression in HT-29 cells, suggesting that vitamin A may be required for proper in vivo regulation of IgA transport in response to mucosal infections.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1063-1069 |
| Number of pages | 7 |
| Journal | Journal of Nutrition |
| Volume | 128 |
| Issue number | 7 |
| State | Published - Jul 1998 |
| Externally published | Yes |
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Keywords
- Antibodies
- Humans
- Retinoic acid
- Transporters
- Vitamin A
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Food Science
Cite this
Vitamin A is required for regulation of polymeric immunoglobulin receptor (plgR) expression by interleukin-4 and interferon-γ in a human intestinal epithelial cell line. / Sarkar, Jolly; Gangopadhyay, Nupur N.; Moldoveanu, Zina; Mestecky, Jiri; Stephensen, Charles B.
In: Journal of Nutrition, Vol. 128, No. 7, 07.1998, p. 1063-1069.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Vitamin A is required for regulation of polymeric immunoglobulin receptor (plgR) expression by interleukin-4 and interferon-γ in a human intestinal epithelial cell line
AU - Sarkar, Jolly
AU - Gangopadhyay, Nupur N.
AU - Moldoveanu, Zina
AU - Mestecky, Jiri
AU - Stephensen, Charles B.
PY - 1998/7
Y1 - 1998/7
N2 - The secretory immunoglobulin A (IgA) antibody response to infections of mucosal surfaces requires transport of IgA from the basal to apical surface of mucosal epithelial cells by a specific transport protein, the polymeric immunoglobulin receptor (plgR). We have tested the hypothesis that the vitamin A metabolite all-trans retinoic acid (RA) is required for the regulation of plgR expression by the cytokines interleukin-4 (IL-4) and interferon γ (IFN-γ) in HT-29 cells, a well-differentiated human epithelial cell line derived from a colonic carcinoma. plgR expression is upregulated by IFN-γ and IL-4 when HT-29 cells are grown in normal media, but this upregulation was significantly lower when cells were grown in vitamin A- depleted media. Treatment with RA at concentrations from 10-9 to 10-5 mol/L restored normal levels of plgR expression. The percentages of cells expressing cell-surface plgR after 24, 48 and 72 h of treatment with RA, IL- 4 and IFN-γ were 66 ± 10, 90 ± 5 and 92 ± 1, respectively, significantly higher than the percentages seen without RA treatment, which were 32 ± 2.3, 72 ± 1.2 and 30 ± 7, respectively. In addition, the intensity of fluorescence of plgR-positive cells was significantly higher in the RA- treated cultures than in the cultures without RA treatment. Similarly, plgR mRNA levels (adjusted for β-actin mRNA levels) in RA-supplemented cultures were 404, 105 and 949% higher at 24, 48 and 72 h, respectively, than were plgR mRNA levels in identical cultures grown in the absence of RA. These data indicate that RA strongly interacts with IL-4 and IFN-γ to regulate plgR expression in HT-29 cells, suggesting that vitamin A may be required for proper in vivo regulation of IgA transport in response to mucosal infections.
AB - The secretory immunoglobulin A (IgA) antibody response to infections of mucosal surfaces requires transport of IgA from the basal to apical surface of mucosal epithelial cells by a specific transport protein, the polymeric immunoglobulin receptor (plgR). We have tested the hypothesis that the vitamin A metabolite all-trans retinoic acid (RA) is required for the regulation of plgR expression by the cytokines interleukin-4 (IL-4) and interferon γ (IFN-γ) in HT-29 cells, a well-differentiated human epithelial cell line derived from a colonic carcinoma. plgR expression is upregulated by IFN-γ and IL-4 when HT-29 cells are grown in normal media, but this upregulation was significantly lower when cells were grown in vitamin A- depleted media. Treatment with RA at concentrations from 10-9 to 10-5 mol/L restored normal levels of plgR expression. The percentages of cells expressing cell-surface plgR after 24, 48 and 72 h of treatment with RA, IL- 4 and IFN-γ were 66 ± 10, 90 ± 5 and 92 ± 1, respectively, significantly higher than the percentages seen without RA treatment, which were 32 ± 2.3, 72 ± 1.2 and 30 ± 7, respectively. In addition, the intensity of fluorescence of plgR-positive cells was significantly higher in the RA- treated cultures than in the cultures without RA treatment. Similarly, plgR mRNA levels (adjusted for β-actin mRNA levels) in RA-supplemented cultures were 404, 105 and 949% higher at 24, 48 and 72 h, respectively, than were plgR mRNA levels in identical cultures grown in the absence of RA. These data indicate that RA strongly interacts with IL-4 and IFN-γ to regulate plgR expression in HT-29 cells, suggesting that vitamin A may be required for proper in vivo regulation of IgA transport in response to mucosal infections.
KW - Antibodies
KW - Humans
KW - Retinoic acid
KW - Transporters
KW - Vitamin A
UR - http://www.scopus.com/inward/record.url?scp=0031820805&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031820805&partnerID=8YFLogxK
M3 - Article
C2 - 9649586
AN - SCOPUS:0031820805
VL - 128
SP - 1063
EP - 1069
JO - Journal of Nutrition
JF - Journal of Nutrition
SN - 0022-3166
IS - 7
ER -