Visualizing regulatory T cell control of autoimmune responses in nonobese diabetic mice

Qizhi Tang, Jason Yeates Adams, Aaron J. Tooley, Mingying Bi, Brian T. Fife, Pau Serra, Pere Santamaria, Richard M. Locksley, Matthew F. Krummel, Jeffrey A. Bluestone

Research output: Contribution to journalArticle

626 Citations (Scopus)

Abstract

The in vivo mechanism of regulatory T cell (Treg cell) function in controlling autoimmunity remains controversial. Here we have used two-photon laser-scanning microscopy to analyze lymph node priming of diabetogenic T cells and to delineate the mechanisms of Treg cell control of autoimmunity in vivo. Islet antigen -specific CD4+CD25- T helper cells (TH cells) and Treg cells swarmed and arrested in the presence of autoantigens. These TH cell activities were progressively inhibited in the presence of increasing numbers of Treg cells. There were no detectable stable associations between Treg and TH cells during active suppression. In contrast, Treg cells directly interacted with dendritic cells bearing islet antigen. Such persistent Treg cell-dendritic cell contacts preceded the inhibition of TH cell activation by dendritic cells, supporting the idea that dendritic cells are central to Treg cell function in vivo.

Original languageEnglish (US)
Pages (from-to)83-92
Number of pages10
JournalNature Immunology
Volume7
Issue number1
DOIs
StatePublished - Jan 2006

Fingerprint

Inbred NOD Mouse
Regulatory T-Lymphocytes
Autoimmunity
Helper-Inducer T-Lymphocytes
Dendritic Cells
Contact Inhibition
CD4 Antigens
Autoantigens
Photons
Confocal Microscopy
Lymph Nodes
T-Lymphocytes
Antigens

ASJC Scopus subject areas

  • Immunology

Cite this

Tang, Q., Adams, J. Y., Tooley, A. J., Bi, M., Fife, B. T., Serra, P., ... Bluestone, J. A. (2006). Visualizing regulatory T cell control of autoimmune responses in nonobese diabetic mice. Nature Immunology, 7(1), 83-92. https://doi.org/10.1038/ni1289

Visualizing regulatory T cell control of autoimmune responses in nonobese diabetic mice. / Tang, Qizhi; Adams, Jason Yeates; Tooley, Aaron J.; Bi, Mingying; Fife, Brian T.; Serra, Pau; Santamaria, Pere; Locksley, Richard M.; Krummel, Matthew F.; Bluestone, Jeffrey A.

In: Nature Immunology, Vol. 7, No. 1, 01.2006, p. 83-92.

Research output: Contribution to journalArticle

Tang, Q, Adams, JY, Tooley, AJ, Bi, M, Fife, BT, Serra, P, Santamaria, P, Locksley, RM, Krummel, MF & Bluestone, JA 2006, 'Visualizing regulatory T cell control of autoimmune responses in nonobese diabetic mice', Nature Immunology, vol. 7, no. 1, pp. 83-92. https://doi.org/10.1038/ni1289
Tang, Qizhi ; Adams, Jason Yeates ; Tooley, Aaron J. ; Bi, Mingying ; Fife, Brian T. ; Serra, Pau ; Santamaria, Pere ; Locksley, Richard M. ; Krummel, Matthew F. ; Bluestone, Jeffrey A. / Visualizing regulatory T cell control of autoimmune responses in nonobese diabetic mice. In: Nature Immunology. 2006 ; Vol. 7, No. 1. pp. 83-92.
@article{2a7be08773a84848a940313409a131a5,
title = "Visualizing regulatory T cell control of autoimmune responses in nonobese diabetic mice",
abstract = "The in vivo mechanism of regulatory T cell (Treg cell) function in controlling autoimmunity remains controversial. Here we have used two-photon laser-scanning microscopy to analyze lymph node priming of diabetogenic T cells and to delineate the mechanisms of Treg cell control of autoimmunity in vivo. Islet antigen -specific CD4+CD25- T helper cells (TH cells) and Treg cells swarmed and arrested in the presence of autoantigens. These TH cell activities were progressively inhibited in the presence of increasing numbers of Treg cells. There were no detectable stable associations between Treg and TH cells during active suppression. In contrast, Treg cells directly interacted with dendritic cells bearing islet antigen. Such persistent Treg cell-dendritic cell contacts preceded the inhibition of TH cell activation by dendritic cells, supporting the idea that dendritic cells are central to Treg cell function in vivo.",
author = "Qizhi Tang and Adams, {Jason Yeates} and Tooley, {Aaron J.} and Mingying Bi and Fife, {Brian T.} and Pau Serra and Pere Santamaria and Locksley, {Richard M.} and Krummel, {Matthew F.} and Bluestone, {Jeffrey A.}",
year = "2006",
month = "1",
doi = "10.1038/ni1289",
language = "English (US)",
volume = "7",
pages = "83--92",
journal = "Nature Immunology",
issn = "1529-2908",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Visualizing regulatory T cell control of autoimmune responses in nonobese diabetic mice

AU - Tang, Qizhi

AU - Adams, Jason Yeates

AU - Tooley, Aaron J.

AU - Bi, Mingying

AU - Fife, Brian T.

AU - Serra, Pau

AU - Santamaria, Pere

AU - Locksley, Richard M.

AU - Krummel, Matthew F.

AU - Bluestone, Jeffrey A.

PY - 2006/1

Y1 - 2006/1

N2 - The in vivo mechanism of regulatory T cell (Treg cell) function in controlling autoimmunity remains controversial. Here we have used two-photon laser-scanning microscopy to analyze lymph node priming of diabetogenic T cells and to delineate the mechanisms of Treg cell control of autoimmunity in vivo. Islet antigen -specific CD4+CD25- T helper cells (TH cells) and Treg cells swarmed and arrested in the presence of autoantigens. These TH cell activities were progressively inhibited in the presence of increasing numbers of Treg cells. There were no detectable stable associations between Treg and TH cells during active suppression. In contrast, Treg cells directly interacted with dendritic cells bearing islet antigen. Such persistent Treg cell-dendritic cell contacts preceded the inhibition of TH cell activation by dendritic cells, supporting the idea that dendritic cells are central to Treg cell function in vivo.

AB - The in vivo mechanism of regulatory T cell (Treg cell) function in controlling autoimmunity remains controversial. Here we have used two-photon laser-scanning microscopy to analyze lymph node priming of diabetogenic T cells and to delineate the mechanisms of Treg cell control of autoimmunity in vivo. Islet antigen -specific CD4+CD25- T helper cells (TH cells) and Treg cells swarmed and arrested in the presence of autoantigens. These TH cell activities were progressively inhibited in the presence of increasing numbers of Treg cells. There were no detectable stable associations between Treg and TH cells during active suppression. In contrast, Treg cells directly interacted with dendritic cells bearing islet antigen. Such persistent Treg cell-dendritic cell contacts preceded the inhibition of TH cell activation by dendritic cells, supporting the idea that dendritic cells are central to Treg cell function in vivo.

UR - http://www.scopus.com/inward/record.url?scp=29244482759&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=29244482759&partnerID=8YFLogxK

U2 - 10.1038/ni1289

DO - 10.1038/ni1289

M3 - Article

VL - 7

SP - 83

EP - 92

JO - Nature Immunology

JF - Nature Immunology

SN - 1529-2908

IS - 1

ER -