Visualizing regulatory T cell control of autoimmune responses in nonobese diabetic mice

Qizhi Tang, Jason Y. Adams, Aaron J. Tooley, Mingying Bi, Brian T. Fife, Pau Serra, Pere Santamaria, Richard M. Locksley, Matthew F. Krummel, Jeffrey A. Bluestone

Research output: Contribution to journalArticlepeer-review

647 Scopus citations


The in vivo mechanism of regulatory T cell (Treg cell) function in controlling autoimmunity remains controversial. Here we have used two-photon laser-scanning microscopy to analyze lymph node priming of diabetogenic T cells and to delineate the mechanisms of Treg cell control of autoimmunity in vivo. Islet antigen -specific CD4+CD25- T helper cells (TH cells) and Treg cells swarmed and arrested in the presence of autoantigens. These TH cell activities were progressively inhibited in the presence of increasing numbers of Treg cells. There were no detectable stable associations between Treg and TH cells during active suppression. In contrast, Treg cells directly interacted with dendritic cells bearing islet antigen. Such persistent Treg cell-dendritic cell contacts preceded the inhibition of TH cell activation by dendritic cells, supporting the idea that dendritic cells are central to Treg cell function in vivo.

Original languageEnglish (US)
Pages (from-to)83-92
Number of pages10
JournalNature Immunology
Issue number1
StatePublished - Jan 2006
Externally publishedYes

ASJC Scopus subject areas

  • Immunology


Dive into the research topics of 'Visualizing regulatory T cell control of autoimmune responses in nonobese diabetic mice'. Together they form a unique fingerprint.

Cite this