Visualization of Rad54, a Chromatin Remodeling Protein, Translocating on Single DNA Molecules

Ichiro Amitani, Ronald J. Baskin, Stephen C. Kowalczykowski

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Rad54 protein plays an important role in the recombinational repair of double-strand DNA (dsDNA) breaks. It is a dsDNA-dependent ATPase that belongs to the Swi2/Snf2 family of chromatin-remodeling proteins. Rad54 remodels (1) DNA structure, (2) chromatin structure, and (3) Rad51-dsDNA complexes. These abilities imply that Rad54 moves along DNA. Here, we provide direct evidence of Rad54 translocation by visualizing its movement along single molecules of dsDNA. When compared to the remodeling processes, translocation is unexpectedly rapid, occurring at 301 ± 22 bp/s at 25°C. Rad54 binds randomly along the dsDNA and moves in either of the two possible directions with a velocity dependent on ATP concentration (Km = 97 ± 28 μM). Movement is also surprisingly processive: the average distance traveled is ∼11,500 bp, with molecules traversing up to 32,000 bp before stopping. The mechanistic implications of this vigorous Rad54 translocase activity in chromatin and protein-DNA complex remodeling are discussed.

Original languageEnglish (US)
Pages (from-to)143-148
Number of pages6
JournalMolecular Cell
Volume23
Issue number1
DOIs
StatePublished - Jul 7 2006

Fingerprint

Chromatin Assembly and Disassembly
Chromatin
DNA
Proteins
Adenosine Triphosphatases
Adenosine Triphosphate

Keywords

  • DNA

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Visualization of Rad54, a Chromatin Remodeling Protein, Translocating on Single DNA Molecules. / Amitani, Ichiro; Baskin, Ronald J.; Kowalczykowski, Stephen C.

In: Molecular Cell, Vol. 23, No. 1, 07.07.2006, p. 143-148.

Research output: Contribution to journalArticle

Amitani, Ichiro ; Baskin, Ronald J. ; Kowalczykowski, Stephen C. / Visualization of Rad54, a Chromatin Remodeling Protein, Translocating on Single DNA Molecules. In: Molecular Cell. 2006 ; Vol. 23, No. 1. pp. 143-148.
@article{3890c037554542daa4f417bd1184d6bc,
title = "Visualization of Rad54, a Chromatin Remodeling Protein, Translocating on Single DNA Molecules",
abstract = "Rad54 protein plays an important role in the recombinational repair of double-strand DNA (dsDNA) breaks. It is a dsDNA-dependent ATPase that belongs to the Swi2/Snf2 family of chromatin-remodeling proteins. Rad54 remodels (1) DNA structure, (2) chromatin structure, and (3) Rad51-dsDNA complexes. These abilities imply that Rad54 moves along DNA. Here, we provide direct evidence of Rad54 translocation by visualizing its movement along single molecules of dsDNA. When compared to the remodeling processes, translocation is unexpectedly rapid, occurring at 301 ± 22 bp/s at 25°C. Rad54 binds randomly along the dsDNA and moves in either of the two possible directions with a velocity dependent on ATP concentration (Km = 97 ± 28 μM). Movement is also surprisingly processive: the average distance traveled is ∼11,500 bp, with molecules traversing up to 32,000 bp before stopping. The mechanistic implications of this vigorous Rad54 translocase activity in chromatin and protein-DNA complex remodeling are discussed.",
keywords = "DNA",
author = "Ichiro Amitani and Baskin, {Ronald J.} and Kowalczykowski, {Stephen C.}",
year = "2006",
month = "7",
day = "7",
doi = "10.1016/j.molcel.2006.05.009",
language = "English (US)",
volume = "23",
pages = "143--148",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "1",

}

TY - JOUR

T1 - Visualization of Rad54, a Chromatin Remodeling Protein, Translocating on Single DNA Molecules

AU - Amitani, Ichiro

AU - Baskin, Ronald J.

AU - Kowalczykowski, Stephen C.

PY - 2006/7/7

Y1 - 2006/7/7

N2 - Rad54 protein plays an important role in the recombinational repair of double-strand DNA (dsDNA) breaks. It is a dsDNA-dependent ATPase that belongs to the Swi2/Snf2 family of chromatin-remodeling proteins. Rad54 remodels (1) DNA structure, (2) chromatin structure, and (3) Rad51-dsDNA complexes. These abilities imply that Rad54 moves along DNA. Here, we provide direct evidence of Rad54 translocation by visualizing its movement along single molecules of dsDNA. When compared to the remodeling processes, translocation is unexpectedly rapid, occurring at 301 ± 22 bp/s at 25°C. Rad54 binds randomly along the dsDNA and moves in either of the two possible directions with a velocity dependent on ATP concentration (Km = 97 ± 28 μM). Movement is also surprisingly processive: the average distance traveled is ∼11,500 bp, with molecules traversing up to 32,000 bp before stopping. The mechanistic implications of this vigorous Rad54 translocase activity in chromatin and protein-DNA complex remodeling are discussed.

AB - Rad54 protein plays an important role in the recombinational repair of double-strand DNA (dsDNA) breaks. It is a dsDNA-dependent ATPase that belongs to the Swi2/Snf2 family of chromatin-remodeling proteins. Rad54 remodels (1) DNA structure, (2) chromatin structure, and (3) Rad51-dsDNA complexes. These abilities imply that Rad54 moves along DNA. Here, we provide direct evidence of Rad54 translocation by visualizing its movement along single molecules of dsDNA. When compared to the remodeling processes, translocation is unexpectedly rapid, occurring at 301 ± 22 bp/s at 25°C. Rad54 binds randomly along the dsDNA and moves in either of the two possible directions with a velocity dependent on ATP concentration (Km = 97 ± 28 μM). Movement is also surprisingly processive: the average distance traveled is ∼11,500 bp, with molecules traversing up to 32,000 bp before stopping. The mechanistic implications of this vigorous Rad54 translocase activity in chromatin and protein-DNA complex remodeling are discussed.

KW - DNA

UR - http://www.scopus.com/inward/record.url?scp=33745498749&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745498749&partnerID=8YFLogxK

U2 - 10.1016/j.molcel.2006.05.009

DO - 10.1016/j.molcel.2006.05.009

M3 - Article

C2 - 16818238

AN - SCOPUS:33745498749

VL - 23

SP - 143

EP - 148

JO - Molecular Cell

JF - Molecular Cell

SN - 1097-2765

IS - 1

ER -