Visualization of epithelial morphology using autofluorescence microscopy under ultraviolet excitation

Bevin Lin; Shiro Urayama; Ramez M G Saroufeem; Dennis L Matthews; Stavros G. Demos

doi:10.1117/12.840715

Visualization of epithelial morphology using autofluorescence microscopy under ultraviolet excitation

Bevin Lin, Shiro Urayama, Ramez M G Saroufeem, Dennis L Matthews, Stavros G. Demos

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Epithelial microstructures related to early stage carcinoma are currently invisible to traditional white light endoscopy. We recently demonstrated that autofluorescence microscopy under ultraviolet excitation can visualize superficial microstructures without the use of contrast agents, sectioning methods, or tissue preparation. Spectroscopic analysis allowed a better understanding of autofluorescence signal characteristics at the microscopic level and the mechanism for achieving high quality imaging of the superficial epithelial layer with conventional wide-field microscopy. The designing parameters for the adaptation of this technology into an endoscope probe for real-time in vivo microscopy are tested using a bench-top prototype system. This approach may provide a powerful tool for the detection and staging of carcinomas.

Original language	English (US)
Title of host publication	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	7561
DOIs	https://doi.org/10.1117/12.840715
State	Published - 2010
Event	Optical Biopsy VII - San Francisco, CA, United States Duration: Jan 25 2010 → Jan 28 2010

Other

Other	Optical Biopsy VII
Country	United States
City	San Francisco, CA
Period	1/25/10 → 1/28/10

Keywords

Autofluorescence
Endoscopy
Gastrointestinal
Microscopy
Real-time diagnostic imaging

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Biomaterials
Radiology Nuclear Medicine and imaging

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10.1117/12.840715

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Visualization of epithelial morphology using autofluorescence microscopy under ultraviolet excitation. / Lin, Bevin; Urayama, Shiro; Saroufeem, Ramez M G; Matthews, Dennis L; Demos, Stavros G.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7561 2010. 756103.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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title = "Visualization of epithelial morphology using autofluorescence microscopy under ultraviolet excitation",

abstract = "Epithelial microstructures related to early stage carcinoma are currently invisible to traditional white light endoscopy. We recently demonstrated that autofluorescence microscopy under ultraviolet excitation can visualize superficial microstructures without the use of contrast agents, sectioning methods, or tissue preparation. Spectroscopic analysis allowed a better understanding of autofluorescence signal characteristics at the microscopic level and the mechanism for achieving high quality imaging of the superficial epithelial layer with conventional wide-field microscopy. The designing parameters for the adaptation of this technology into an endoscope probe for real-time in vivo microscopy are tested using a bench-top prototype system. This approach may provide a powerful tool for the detection and staging of carcinomas.",

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author = "Bevin Lin and Shiro Urayama and Saroufeem, {Ramez M G} and Matthews, {Dennis L} and Demos, {Stavros G.}",

year = "2010",

doi = "10.1117/12.840715",

language = "English (US)",

isbn = "9780819479570",

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note = "Optical Biopsy VII ; Conference date: 25-01-2010 Through 28-01-2010",

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AU - Lin, Bevin

AU - Urayama, Shiro

AU - Saroufeem, Ramez M G

AU - Matthews, Dennis L

AU - Demos, Stavros G.

PY - 2010

Y1 - 2010

N2 - Epithelial microstructures related to early stage carcinoma are currently invisible to traditional white light endoscopy. We recently demonstrated that autofluorescence microscopy under ultraviolet excitation can visualize superficial microstructures without the use of contrast agents, sectioning methods, or tissue preparation. Spectroscopic analysis allowed a better understanding of autofluorescence signal characteristics at the microscopic level and the mechanism for achieving high quality imaging of the superficial epithelial layer with conventional wide-field microscopy. The designing parameters for the adaptation of this technology into an endoscope probe for real-time in vivo microscopy are tested using a bench-top prototype system. This approach may provide a powerful tool for the detection and staging of carcinomas.

AB - Epithelial microstructures related to early stage carcinoma are currently invisible to traditional white light endoscopy. We recently demonstrated that autofluorescence microscopy under ultraviolet excitation can visualize superficial microstructures without the use of contrast agents, sectioning methods, or tissue preparation. Spectroscopic analysis allowed a better understanding of autofluorescence signal characteristics at the microscopic level and the mechanism for achieving high quality imaging of the superficial epithelial layer with conventional wide-field microscopy. The designing parameters for the adaptation of this technology into an endoscope probe for real-time in vivo microscopy are tested using a bench-top prototype system. This approach may provide a powerful tool for the detection and staging of carcinomas.

KW - Autofluorescence

KW - Endoscopy

KW - Gastrointestinal

KW - Microscopy

KW - Real-time diagnostic imaging

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DO - 10.1117/12.840715

M3 - Conference contribution

AN - SCOPUS:77951735627

SN - 9780819479570

VL - 7561

BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

T2 - Optical Biopsy VII

Y2 - 25 January 2010 through 28 January 2010

ER -

Visualization of epithelial morphology using autofluorescence microscopy under ultraviolet excitation

Abstract

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Keywords

ASJC Scopus subject areas

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