Visualization of epithelial morphology using autofluorescence microscopy under ultraviolet excitation

Bevin Lin, Shiro Urayama, Ramez M G Saroufeem, Dennis L Matthews, Stavros G. Demos

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Epithelial microstructures related to early stage carcinoma are currently invisible to traditional white light endoscopy. We recently demonstrated that autofluorescence microscopy under ultraviolet excitation can visualize superficial microstructures without the use of contrast agents, sectioning methods, or tissue preparation. Spectroscopic analysis allowed a better understanding of autofluorescence signal characteristics at the microscopic level and the mechanism for achieving high quality imaging of the superficial epithelial layer with conventional wide-field microscopy. The designing parameters for the adaptation of this technology into an endoscope probe for real-time in vivo microscopy are tested using a bench-top prototype system. This approach may provide a powerful tool for the detection and staging of carcinomas.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7561
DOIs
StatePublished - 2010
EventOptical Biopsy VII - San Francisco, CA, United States
Duration: Jan 25 2010Jan 28 2010

Other

OtherOptical Biopsy VII
CountryUnited States
CitySan Francisco, CA
Period1/25/101/28/10

Keywords

  • Autofluorescence
  • Endoscopy
  • Gastrointestinal
  • Microscopy
  • Real-time diagnostic imaging

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Lin, B., Urayama, S., Saroufeem, R. M. G., Matthews, D. L., & Demos, S. G. (2010). Visualization of epithelial morphology using autofluorescence microscopy under ultraviolet excitation. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7561). [756103] https://doi.org/10.1117/12.840715