Visualization of epithelial morphology using autofluorescence microscopy under ultraviolet excitation

Bevin Lin; Shiro Urayama; Ramez M G Saroufeem; Dennis L Matthews; Stavros G. Demos

doi:10.1117/12.840715

Visualization of epithelial morphology using autofluorescence microscopy under ultraviolet excitation

Bevin Lin, Shiro Urayama, Ramez M G Saroufeem, Dennis L Matthews, Stavros G. Demos

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Epithelial microstructures related to early stage carcinoma are currently invisible to traditional white light endoscopy. We recently demonstrated that autofluorescence microscopy under ultraviolet excitation can visualize superficial microstructures without the use of contrast agents, sectioning methods, or tissue preparation. Spectroscopic analysis allowed a better understanding of autofluorescence signal characteristics at the microscopic level and the mechanism for achieving high quality imaging of the superficial epithelial layer with conventional wide-field microscopy. The designing parameters for the adaptation of this technology into an endoscope probe for real-time in vivo microscopy are tested using a bench-top prototype system. This approach may provide a powerful tool for the detection and staging of carcinomas.

Original language	English (US)
Title of host publication	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	7561
DOIs	https://doi.org/10.1117/12.840715
State	Published - 2010
Event	Optical Biopsy VII - San Francisco, CA, United States Duration: Jan 25 2010 → Jan 28 2010

Other

Other	Optical Biopsy VII
Country	United States
City	San Francisco, CA
Period	1/25/10 → 1/28/10

Keywords

Autofluorescence
Endoscopy
Gastrointestinal
Microscopy
Real-time diagnostic imaging

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Biomaterials
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.840715

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Visualization of epithelial morphology using autofluorescence microscopy under ultraviolet excitation. / Lin, Bevin; Urayama, Shiro; Saroufeem, Ramez M G; Matthews, Dennis L; Demos, Stavros G.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7561 2010. 756103.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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abstract = "Epithelial microstructures related to early stage carcinoma are currently invisible to traditional white light endoscopy. We recently demonstrated that autofluorescence microscopy under ultraviolet excitation can visualize superficial microstructures without the use of contrast agents, sectioning methods, or tissue preparation. Spectroscopic analysis allowed a better understanding of autofluorescence signal characteristics at the microscopic level and the mechanism for achieving high quality imaging of the superficial epithelial layer with conventional wide-field microscopy. The designing parameters for the adaptation of this technology into an endoscope probe for real-time in vivo microscopy are tested using a bench-top prototype system. This approach may provide a powerful tool for the detection and staging of carcinomas.",

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