Abstract
Epithelial microstructures related to early stage carcinoma are currently invisible to traditional white light endoscopy. We recently demonstrated that autofluorescence microscopy under ultraviolet excitation can visualize superficial microstructures without the use of contrast agents, sectioning methods, or tissue preparation. Spectroscopic analysis allowed a better understanding of autofluorescence signal characteristics at the microscopic level and the mechanism for achieving high quality imaging of the superficial epithelial layer with conventional wide-field microscopy. The designing parameters for the adaptation of this technology into an endoscope probe for real-time in vivo microscopy are tested using a bench-top prototype system. This approach may provide a powerful tool for the detection and staging of carcinomas.
Original language | English (US) |
---|---|
Title of host publication | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
Volume | 7561 |
DOIs | |
State | Published - 2010 |
Event | Optical Biopsy VII - San Francisco, CA, United States Duration: Jan 25 2010 → Jan 28 2010 |
Other
Other | Optical Biopsy VII |
---|---|
Country | United States |
City | San Francisco, CA |
Period | 1/25/10 → 1/28/10 |
Keywords
- Autofluorescence
- Endoscopy
- Gastrointestinal
- Microscopy
- Real-time diagnostic imaging
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Radiology Nuclear Medicine and imaging