Visualization of chorioretinal vasculature in mice in vivo using a combined OCT/SLO imaging system

Mayank Goswami, Pengfei Zhang, Edward N Pugh Jr, Robert Zawadzki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Chorioretinal blood vessel morphology in mice is of great interest to researchers studying eye disease mechanisms in animal models. Two leading retinal imaging modalities - Optical Coherence Tomography (OCT) and Scanning Laser Ophthalmoscopy (SLO) - have offered much insight into vascular morphology and blood flow. OCT "flow-contrast" methods have provided detailed mapping of vascular morphology with micrometer depth resolution, while OCT Doppler methods have enabled the measurement of local flow velocities. SLO remains indispensable in studying blood leakage, microaneurysms, and the clearance time of contrast agents of different sizes. In this manuscript we present results obtained with a custom OCT/SLO system applied to visualize the chorioretinal vascular morphology of pigmented C57Bl/6J and albino nude (Nu/Nu) mice. Blood perfusion maps of choroidal vessels and choricapillaris created by OCT and SLO are presented, along with detailed evaluation of different OCT imaging parameters, including the use of the scattering contrast agent Intralipid. Future applications are discussed.

Original languageEnglish (US)
Title of host publicationOphthalmic Technologies XXVI
PublisherSPIE
Volume9693
ISBN (Electronic)9781628419276
DOIs
StatePublished - 2016
Event26th Conference on Ophthalmic Technologies - San Francisco, United States
Duration: Feb 13 2016Feb 14 2016

Other

Other26th Conference on Ophthalmic Technologies
CountryUnited States
CitySan Francisco
Period2/13/162/14/16

Keywords

  • blood flow
  • choroidal vasculature
  • in vivo imaging
  • mouse retina
  • OCT angiography
  • Optical Coherence Tomography
  • Scanning Laser Ophthalmoscopy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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